DOI QR코드

DOI QR Code

기장산과 완도산 건 다시마의 무기성분 및 생리활성 분석

Mineral Contents and Physiological Activities of Dried Sea Tangle (Laminaria japonica) Collected from Gijang and Wando in Korea.

  • 발행 : 2008.04.30

초록

시판되는 기장 및 완도 다시마 완제품(건 다시마)의 일반성분, 미량금속, 유리아미노산 함량, 알긴산 함량, 항산화 효과와 항염증 효과를 조사하여 산지별 다시마의 생리활성을 비교, 분석하였다. 일반성분 중, 기장 다시마와 완도 다시마 간의 수분, 회분, 조지방, 탄수화물, 열량의 값은 큰 차이를 보이지는 않았지만 조단백의 경우, 기장 다시마가 8.41%, 완도 다시마가 7.25%로 나타나 기장 다시마의 조단백 함량이 완도 다시마의 조단백 함량보다 다소 높은 것으로 나타났다. 주요 유리아미노산은 glutamic acid, aspartic acid, alanine, proline 및 hydroxyproline이었으며, 유리아미노산의 함량을 비교한 결과, threonine, valine, 그리고 lysine 경우는 기장 다시마에서 더 높은 함량을 보였고, methionine, leucine, phenylalanine 및 histidine은 완도 다시마에서 더 높은 함량을 보였다. 비 필수아미노산의 함량은 기장 다시마의 함량이 완도 다시마에 비해 전반적으로 높게 나타났다. 4종류의 주요 금속원소(Na, K, Ca, Mg)와 3종류의 미량금속원소(Fe, Zn, P)의 농도를 비교한 결과, Fe의 함량은 완도 다시마가 높게 나타났으며, Na, K, Ca, Zn, Mg 그리고 P의 함량은 기장 다시마가 모두 높게 나타났다. 알긴산의 함량은 기장 다시마의 알긴산 함량(19.8%)이 완도 다시마(17.9%)보다 다소 높은 것으로 나타났으며, DPPH radical scavenging 활성은 완도 다시마의 전자공여능이 기장 다시마의 전자 공여능보다 약 17.8% 정도 높은 것으로 났다. SOD 유사활성은 완도 다시마(49.7%)가 기장 다시마(46.2%)보다 다소 높은 SOD 유사활성을 나타냈다. LPS에 의하여 유도된 NO 합성은 다시마 methanol 추출물을 처리함으로서 NO 합성이 $200\;{\mu}g/ml$농도에서 기장 및 완도 다시마의 NO 합성저해활성은 각각 21.4%와 32.6%로 나타났다. 이러한 결과는 다시마 methanol 추출물이 면역기능과 밀접한 관계가 있음을 나타내는 것이다.

This research was performed to determine the proximate compositions, mineral contents, alginic acid, antioxidative activities and amino acids of sea tangles collected from Gijang and Wando area. Crude protein and ash contents were higher in Gijang sea tangle, whereas carbohydrate and moisture were higher in Wando in general. Mineral contents of Gijang sea tangle were higher than Wando. Especially, Na and K was the most abundant in both Gijang and Wando sea tangles. Alginic acid content was almost similar in both sea tangles. The major free amino acids were glutamic acid, aspartic acid, alanine, proline and hydroxyproline in both Gijang and Wando sea tangles. Antioxidative activity of methanol extract of sea tangle was measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging and SOD-like activity were about 17% ($40\;{\mu}g/ml$) and 7% ($5\;{\mu}g/ml$) higher, respectively, in Wando sea tangle. When stimulate the macrophages RAW264.7 cells with lipopolysaccharide (LPS), inhibition of NO synthesis of the methanol extract was 11% higher in Wando sea tangle comparing with Gijang samples.

키워드

참고문헌

  1. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200 https://doi.org/10.1038/1811199a0
  2. Bojakowski, K., P. Abramczyk, M. Bojakowska, A. Zwolinska, J. Przybylski and Z. Gaciong. 2001. Fucoidan improves the renal blood flow in the early stage of renal ischemia/ reperfusion injury in the rat. J. Physiol. Pharmacol. 52, 137-143
  3. Cho, D. M., D. S. Kim, D. S. Lee, H. R. Kim and J. H. Pyeun. 1995. Trace components and functional saccharides in seaweed-1. Bull. Korean Fish Soc. 28, 49-59
  4. Ciofani, G., V. Raffa, A. Menciassi, S. Micera and P. Dario. 2007. A drug delivery system based on alginate microspheres: mass-transport test and in vivo validation. Biomed. Microdevices 9, 395-403 https://doi.org/10.1007/s10544-006-9044-0
  5. Dawczynski, C., R. Schubert and G. Jahreis. 2007. Amino acids, fatty acids, and dietary fiber in edible seaweed products. Food Chem. 103, 891-899 https://doi.org/10.1016/j.foodchem.2006.09.041
  6. Farias-Eisner, R., M. P. Sherman, E. Aeberhard and G. Chaudhuri. 1994. Nitric oxide is an important mediator for tumoricidal activity in vivo. Proc. Natl. Acad. Sci. 91, 9407-9411 https://doi.org/10.1073/pnas.91.20.9407
  7. Green, L. C., D. A. Wagner, J. Glogowski, P. L. Skipper, J. S. Wishnok and S. R. Tannenbaum. 1982. Analysis of nitrate, nitrite, and [^{15}N$] nitrate in biological fluids. Anal. Biochem. 126, 131-138 https://doi.org/10.1016/0003-2697(82)90118-X
  8. Haroun-Boihedja, F., M. Ellouali, C. Sinquin and C. Boisson-Vidal. 2000. Relationship between sulfate groups and biological activities of fucans. Thrombosis Research 100, 453-459 https://doi.org/10.1016/S0049-3848(00)00338-8
  9. Hong, H. D., N. Kang and S. S. Kim. 1998. Superoxide dismutase-like activity of apple juice mixed with some fruits and vegetables. Korean J. Food Sci. Technol. 30, 1484-1487
  10. Ignarro, L. J., J. M. Fukutto, J. M. Griscavage, N. E. Rogers and R. E. Byrns. 1993. Oxidation of nitric oxide in aqueous solution to nitrite but not nitrite: Comparison with enzymatically formed nitric oxide form L-arginine. Proc. Natl. Acad. Sci. 90, 8103-8107 https://doi.org/10.1073/pnas.90.17.8103
  11. Im, Y. G., J. S. Choi and D. S. Kim. 2006. Mineral contents of edible seaweeds collected from Gijang and Wando in Korea. J. Korean Fish Soc. 39, 16-22 https://doi.org/10.5657/kfas.2006.39.1.016
  12. Jimenez-Escrig, A. and I. Goni Cambrodon. 1999. Nutritional evaluation and physiological effects of edible seaweeds. Arch. Latinoam. Nutr. 49, 114-120
  13. Jin, D. Q., G. Li, J. S. Lim, C. S. Young, J. A. Kim and K. Hun. 2004. Preventive effects of Laminaria japonica aqueous extract on the oxidative stress and xanthine oxidase activity in streptozotocin-induced diabetic rats liver. Biol. Pharm. Bull. 27, 1037-1040 https://doi.org/10.1248/bpb.27.1037
  14. Kang, J. W. and N. P. Ko. 1997. Seaweed Aquaculture pp. 199-202, Taewha Publication, Busan. Korea
  15. Kim, D. S., D. S. Lee, D. M. Cho, H. R. Kim and J. H. Pyeun. 1995. Trace components and functional saccharides in marine algae. 2. Dietary fiber contents and distribution of the algal polysaccharides. J. Korean Fish Soc. 28, 270-278
  16. Kim, J. A. and J. M. Lee. 2004. The change of biologically functional compounds and antioxidant activities in Hizikia fusiformis with drying methods. Korean J. Food Culture 19, 200-208
  17. Kim, K. H., Y. W. Kim, H. B. Kim, B. J. Lee and D. S. Lee. 2006. Anti-apoptotic of laminarion polysaccharides and their enzymatically hydrolyzed oligosaccharides from Laminarin japonica. Biotechnol. Letters 28, 439-446 https://doi.org/10.1007/s10529-005-6177-9
  18. Kim, S. H., M. Y. Kang and S. H. Nam. 2005. Modulatory effects of 21 kinds of medicinal herbs including herba pogostemi (Agastache rugosa) on nitric oxide production in macrophage cell line RAW 264.7 cells. J. Korean Soc. Appl. Biol. Chem. 48, 411-417
  19. Korea Food and Drug Administration. 2005. Food Standards Codex. Korean Foods Industry Association, Seoul. Korea. pp. 1267
  20. Lee, B. H., B. W. Choi, J. H. Chun and B. S. Yu. 1996. Extraction of water soluble antioxidants from seaweeds. J. Korean Ind. F Eng. Chemistry 7, 1069-1077
  21. Lee, D. S., H. R. Kim, D. M. Cho, T. J. Nam and J. H. Pyeun. 1998. Uronate compositions of alginates from the edible brown algae. J. Korean Fish Soc. 31, 1-7
  22. Lee, J. H. and N. J. Sung. 1980. The content of minerals in algae. J. Korean Soc. Food & Nutr. 9, 51-58
  23. Marklund, S. and G. Marklund. 1975. Involvement of superoxide aminoradical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 468-474
  24. Mosmann, T. 1983. Rapid calorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  25. Nathan, C. 1992. Nitric oxide as a secretary product of mammalian cells. FASEB J. 6, 3051-3064 https://doi.org/10.1096/fasebj.6.12.1381691
  26. Nice, D. J., D. S. Robinson and M. A. Jolden. 1995. Characterization of a heat-stable antioxidant co-purified with the superoxide dismutase activity from dried peas. Food Chem. 52, 393-397 https://doi.org/10.1016/0308-8146(95)93288-3
  27. Sosulski, F. W. and A. M. Cadden. 1982. Composition and physiological properties of several sources of dietary fiber. J. Food Sci. 47, 1472-1477 https://doi.org/10.1111/j.1365-2621.1982.tb04964.x
  28. Torel, J., J. Gillard and P. Gillard. 1986. Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry 25, 383-385 https://doi.org/10.1016/S0031-9422(00)85485-0
  29. You, B. J., Y. S. Im, I. H. Jeong and K. H. Lee. 1997. Effect extraction conditions on bile acids binding capacity in vitro of alginate extracted from sea tangle (Laminaria spp.). J. Korean Fish Soc. 30, 31-38
  30. van der Veen, R. C. 2001. Nitric oxide and T cell immunity. Int. Immunophamacol. 1, 1491-1500 https://doi.org/10.1016/S1567-5769(01)00093-5
  31. Wang, J., Q. Zhang, Z. Zhang and Z. Li. 2008. Antioxidant activity of sulfated polysaccharides fractions extracted from Laminaria Japonica. J. Biological Macromol. 42, 127-132 https://doi.org/10.1016/j.ijbiomac.2007.10.003

피인용 문헌

  1. Anti-inflammatory Activity of an Ethanol Extract of Laminaria japonica Root on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells vol.46, pp.6, 2014, https://doi.org/10.9721/KJFST.2014.46.6.729
  2. Bioethanol production from mannitol by a newly isolated bacterium, Enterobacter sp. JMP3 vol.135, 2013, https://doi.org/10.1016/j.biortech.2012.10.012
  3. Effect of Yeast Fermentation on the Antioxidant and Anti-inflammatory Activity of Sea Tangle Water Extract vol.43, pp.2, 2010, https://doi.org/10.5657/kfas.2010.43.2.117
  4. Effects of Lactobacillus brevis BJ20 Fermentation on the Antioxidant and Antiinflammatory Activities of Sea Tangle Saccharina japonica and oyster Crassostrea gigas vol.46, pp.4, 2013, https://doi.org/10.5657/KFAS.2013.0359
  5. Characteristics and in vitro Anti-diabetic Properties of the Korean Rice Wine, Makgeolli Fermented with Laminaria japonica vol.19, pp.2, 2014, https://doi.org/10.3746/pnf.2014.19.2.098
  6. An improved method of protein isolation and proteome analysis with Saccharina japonica (Laminariales) incubated under different pH conditions vol.23, pp.1, 2011, https://doi.org/10.1007/s10811-010-9550-6
  7. Physicochemical Characteristics and Antioxidative Effects of Barley Soybean Paste (Doenjang) Containing Kelp Extracts vol.43, pp.12, 2014, https://doi.org/10.3746/jkfn.2014.43.12.1843
  8. Anti-inflammatory Activity of Ethanol Extract of Undaria pinnatifida Root in RAW 264.7 Cells vol.47, pp.6, 2014, https://doi.org/10.5657/KFAS.2014.0751
  9. Nutritional Characteristics and Screening of Biological Activity of Agarum cribrosum vol.25, pp.4, 2012, https://doi.org/10.9799/ksfan.2012.25.4.842