생명과학회지 (Journal of Life Science)

  • 제18권4호
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  • Pages.435-440
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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분자표지자에 의한 지황 유전집단의 유전적 다양성

Genetic Diversity of Rehmannia glutinosa Genotypes Assessed by Molecular Markers

  • 방경환 (농촌진흥청 인삼약초연구소 인삼과) ;
  • 정종욱 (농업생명공학연구원 유전자원과) ;
  • 김영창 (농촌진흥청 인삼약초연구소 인삼과) ;
  • 이제완 (농촌진흥청 인삼약초연구소 인삼과) ;
  • 김홍식 (충북대학교 식물자원학과) ;
  • 김동휘 (농촌진흥청 인삼약초연구소 인삼과)
  • Bang, Kyong-Hwan (Ginseng & Medicinal Plants Research Institute, RDA) ;
  • Chung, Jong-Wook (National Institute of Agricultural Biotechnology, RDA) ;
  • Kim, Young-Chang (Ginseng & Medicinal Plants Research Institute, RDA) ;
  • Lee, Jei-Wan (Ginseng & Medicinal Plants Research Institute, RDA) ;
  • Kim, Hong-Sig (College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Dong-Hwi (Ginseng & Medicinal Plants Research Institute, RDA)
  • 발행 : 2008.04.30
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초록

RAPD 분석을 이용하여 지황 육성 계통과 지역 수집종 들을 구분할 수 있는 분자표지자를 선발하고, 집단 간, 집단 내 유전적 다양성을 평가하기 위하여 본 실험을 수행하였다. 총 20개의 임의 primer를 이용하여 PCR 한결과, 육성 계통과 수집종 들을 구별할 수 있는 OPA-1 등 10개의 재현성과 다형성이 좋은 프라이머 들을 선발하였다. 특히 OPA-10, OPA-11 및 OPA-19는 고려지황과 지황1호를 다른 계통 및 수집종 들과 구별할 수 있었으며, 이들 프라이머를 이용하여 0.9 kb, 1.2 kb, 1.3 kb 및 1.4 kb등의 육성계통 특이적인 DNA 밴드들을 확보할 수 있었다. 한편 이들의 결과를 토대로 통계처리에 의한 유전분석 결과, 고려지황, 지황1호 및 일본지황은 집단 내 유사도가 높아 다른 집단들과 구별되었다. 결론적으로, RAPD 분석을 통한 결과는 지황의 유전적 다양성 이해와 특정 계통을 다른 계통 및 수집종 들과 구분할 수 있는 방법으로 이용될 수 있다.

Random amplified polymorphic DNA (RAPD) markers were used to identify the genetic diversities among and within varieties and landraces of Rehmannia glutinosa. Polymorphic and reproducible bands were produced by 10 primers out of total 20 primers used in the experiment. In RAPD analysis of the 11 genotypes, 64 fragments out of 73 amplified genomic DNA fragments were polymorphic which represented an average 6.4 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPA-1) to 13 (OPA-11) and varied in size from 200 bp to 1,400 bp. Especially, OPA-10, OPA-11 and OPA-19 primers showed specific bands for varieties of Korea Jiwhang and Jiwhang il ho, which could be useful for discriminating from other varieties and landraces of R. glutinosa. Percentage polymorphism ranged from a minimum of 50% (OPA-1) to a maximum of 100% (OPA-11), with an average of 87.7%. Similarity coefficients were higher in the genotypes of Korea Jiwhang and Jiwhang il ho than in other populations. In cluster analysis, genotypes of Korea Jiwhang, Jiwhang il ho, and Japanese accession were separated from those of other varieties and landraces. Average of genetic diversity within the population $(H_S)$ was 0.110, while average of total genetic diversity $(H_T)$ was 0.229. Across all RAPD makers the $G_{ST}$ value was 0.517, indicating that about 52% of the total genetic variation could be explained by RAPDs differences while the remaining 48% might be attributable to differences among samples. Consequently, RAPD analysis was useful method to discriminate different populations such as domestic varieties and other landraces. The results of the present study will be used to understand the population and evolutionary genetics of R. gllutinosa.

키워드

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