Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Comparison of Arylphorin of Antheraea pernyi with Those of Several Lepidopteran Wild Silkmoths by Western Blot Analysis.

작잠(Antheraea pernyi) arylphorin의 항체를 이용한 수종의 나비목 야생 견사곤충들 간의 면역학적 비교

  • Park, Nam-Sook (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University) ;
  • Kim, Mi-Ae (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University) ;
  • Park, Hyun-Chul (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University) ;
  • Kim, Keun-Ki (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Sang-Mong (Dept. of Life Science & Environmental Biochemistry, College of Natural Resources & Life Science, Pusan National University)
  • 박남숙 (부산대학교 생명자원과학대학 생명환경화학과) ;
  • 김미애 (부산대학교 생명자원과학대학 생명환경화학과) ;
  • 박현철 (부산대학교 생명자원과학대학 생명환경화학과) ;
  • 김근기 (부산대학교 생명자원과학대학 생명환경화학과) ;
  • 진병래 (동아대학교 생명자원과학대학 응용생명공학과) ;
  • 이상몽 (부산대학교 생명자원과학대학 생명환경화학과)
  • Published : 2008.03.31
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Abstract

The occurences of proteins relating to Antheraea pernyi arylphorin in haemolymph, fat body, integument, midgut and silkgland of the wild silkmoths, Antheraea yamamai, Antheraea pernyi, Samia cynthia pryeri and Actias gnoma in the 5th larval instar were investigated by immunoblot analysis using mouse polyclonal antibody against A. pernyi arylphorin as probe. In A. yamamai, A. pernyi, S. cynthia pryeri and A. gnoma, the major immunoreactive antigenic proteins with a molecular weight of 80 KDa against the antisera of the A. pernyi arylphorin were clearly observed in the haemolymph, but in the integument, fat body, midgut and silkgland of the corresponding wild silkmoths the presence of the immunoreactive proteins were very variable. These results suggest that the A. pernyi arylphorin has almost same immunological identity with those of the wild silkmoths, A. yamamai, S. cynthia pryeri and A. gnoma though the distribution of the corresponding antigenic arylphorins is different according to the tissues of the wild silkmoths.

작잠 arylphorin 항 혈청을 사용하여 천잠, 가중나무 산누에 나방 및 옥색긴꼬리 산누에나방의 5령 유충의 혈림프, 피부, 지방체, 중장 및 실샘에 대한 종(species) 공통항원의 존재 여부를 비교${\cdot}$검토하였다. 4개종은 혈림프, 피부, 지방체, 중장 실샘 등 종별 조직에 따라 다소간 검출유무의 차이는 있으나 작잠 arylphorin 항 혈청에 대한 공통항원의 존재는 4종의 야생곤충 모두에서 확인되었으며, 특히 혈림프에서 가장 강력한 항원-항체 반응이 관찰되었다. 이상의 결과에서 A. pernyi arylphorin은 천잠 등 4개의 공시곤충들의 공통 조상유전자로부터 유래한 단백질일 가능성이 높은 것으로 사료된다.

Keywords

References

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