생명과학회지 (Journal of Life Science)

  • 제18권3호
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  • Pages.403-408
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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유자와 탱자 과피 추출물의 항산화 및 면역 활성 효과

Effect of Ethanol Extract from Peel of Citrus junos and Poncirus trifoliata on Antioxidant and Immune Activity.

  • 박준희 (동의대학교 분자생물학과) ;
  • 강병원 (동아대학교 BK21 실버바이오 사업단) ;
  • 김지은 (동아대학교 생명공학과) ;
  • 서민정 (동아대학교 생명공학과) ;
  • 이영춘 (동아대학교 생명공학과) ;
  • 이재헌 (동아대학교 유전공학과) ;
  • 주우홍 (창원대학교 생물학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 임학섭 ((주)천년약속 바이오연구소) ;
  • 정영기 (동아대학교 생명공학과, (주)천년약속 바이오연구소) ;
  • 이복규 (동의대학교 분자생물학과)
  • Park, Joon-Hee (Department of Molecular Biology, Dong-Eui University) ;
  • Kang, Byoung-Won (BK21 Center for Silver-Bio industrialization Project, Dong-A University) ;
  • Kim, Ji-Eun (Department of Biotechnology, Dong-A University) ;
  • Seo, Min-Jeong (Department of Biotechnology, Dong-A University) ;
  • Lee, Young-Choon (Department of Biotechnology, Dong-A University) ;
  • Lee, Jai-Heon (Department of Genetic Engineering, Dong-A University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-eui University) ;
  • Lim, Hak-Seob (Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.) ;
  • Jeong, Yong-Kee (Department of Biotechnology, Dong-A University, Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.) ;
  • Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University)
  • 발행 : 2008.03.31
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초록

본 연구는 탱자(Poncirus trifoliata) 과피의 80% 에탄올 추출물(PTP)에 대한 항산화 활성과 면역 활성을 유자(Citrus junos) 과피 추출물(CJP)과 비교하였다. 총 phenol 함량과 총 flavonoids 함량은 PTP가 각각 $60.75{\pm}1.15$ mg/100 g과 $33.75{\pm}0.15$ mg/100 g이었으며, 이는 CJP에 비해 다소 낮았다. PTP의 항산화 활성은 농도 의존적으로 증가하였으며 CJP의 항산화 활성과 비슷하게 나타났다. 면역반응 중 대식 세포가 생성하는 NO 생성량을 측정한 결과 CJP와 PTP를 단독으로 처리한 경우와 LPS와 혼합하여 처리한 경우 모두 1 mg/ml까지는 대조구와 비교하여 농도 의존적으로 증가하였으나 고농도에서는 감소하는 경향을 나타내었다. 비장세포를 이용한 면역세포 증식능에서 CJP와 PTP를 첨가한 경우, 1 mg/ml까지는 대조구와 비교하여 농도 의존적으로 증가하였으나 고농도에서는 감소하였다.

In this study, we compared with 80% ethanol extracts from peel of Poncirus trifoliata (PTP) and peel of Citrus junos (CJP) against antioxidant and immune activities. Total phenolics and flavonoids contents in PTP extracts were $60.75{\pm}1.15$ and $33.75{\pm}0.15$ mg/l00 g, respectively, and those were lower than CJP extracts. Antioxidant activities of PTP were increased with the more concentration, and were similar to CJP. Antioxidant activities of PTP were increased with increasing of concentration, and were similar to those of CJP. The NO production in macrophage cell lines were increased in a dose-dependent manner, until 5 mg/ml of CJP and 1 mg/ml of PTP compared with control cells, but decreased at higher concentrations. The proliferation of mouse spleen cells were increased in a dose-dependent manner, until 1 mg/ml of CJP and PTP compared with control cells but decreased at higher concentrations. The NO production in macrophage cell lines treated with PTP and CJP were increased in a dose-dependent manner, compared with untreated control cells until the concentrations of $1{\sim}5$ mg/ml (CJP) and 1 mg/ml (PTP) but decreased at higher concentrations than that. The proliferation of mouse spleen cells treated with PTP and CJP were increased in a dose-dependent manner, compared with untreated control cells until the concentration of 1 mg/ml but decreased at higher concentrations than that.

키워드

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