생명과학회지 (Journal of Life Science)

  • 제18권3호
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  • Pages.381-386
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Phase I, phase II 효소 및 ornithine decarboxylase에 미치는 해양심층수의 영향

Effect of Deep Sea Water on Phase I, Phase II and Ornithine Decarboxylase.

  • 손윤희 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연구센터) ;
  • 김미경 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연구센터) ;
  • 장정선 (동국대학교 지역연고산업진흥사업단, 난치병한양방치료연구센터) ;
  • 정은정 (동국대학교 의과대학 약리학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실)
  • Shon, Yun-Hee (Regional Innovation System, Intractable Diseases Research Center, Dongguk University) ;
  • Kim, Mee-Kyung (Regional Innovation System, Intractable Diseases Research Center, Dongguk University) ;
  • Jang, Jung-Sun (Regional Innovation System, Intractable Diseases Research Center, Dongguk University) ;
  • Jung, Eun-Jung (Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University)
  • 발행 : 2008.03.31
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초록

동해에서 취수한 해양심층수의 암예방 효능을 얄아보고자 암발생 억제물질의 생화학적 표식자(biochemical markers)인 CYP 1A2 활성, phase II 효소인 QR과 GST의 활성, GSH 함량 및 ODC 활성에 미치는 영향을 살펴보았다. 해양심층수는 암의 개시단계(initiation)를 유발시키는 것으로 알려진 CYP 1A2 활성을 경도의존적으로 저해시켰다. 해양심층수를 Hepa 1clc7 세포에 경도별$(100{\sim}1,000)$로 처리하였을 때 phase II 생체 해독효소인 QR과 GST의 활성은 최대 20%의 증가를 나타내었고, 외부의 독성물질이나 대사산물로부터 세포를 보호하는 역할을 하는 GSH의 함량은 $26{\sim}40%$의 증가를 나타내었다. 그리고 발암과정의 촉진/진행단계에 관여하는 ODC의 활성은 해양심층수의 경도 800과 1,000에서 20%와 35%의 저해율을 나타내었으며, 경도 1,000을 처리한 군에서는 양성대조군인 DFMO와 같은 저해율을 나타내었다. 그러므로 본 연구 결과에 의하면 동해 해양심층수는 발암과정과 관련된 개시 및 촉진/진행단계를 저해시켜 암예방 효능을 나타낼 것으로 사료된다.

Deep sea water was tested for cancer chemopreventive activity by measuring the activities of ${\beta}-$ naphthoflavone $({\beta}-NF)-induced$ cytochrome P 450 1A2 (CYP 1A2), quinone reductase (QR) and glutathione-S-transferase (GST), glutathione (GSH) levels, and ornithine decarboxylase (ODC) activity. The in vitro incubation of rat liver microsome with deep sea water (a hardness range of $100{\sim}1,000$) showed a hardness-dependent inhibition of CYP 1A2 activity. QR and GST activities were induced about $1.1{\sim}1.2$ fold with the treatment of deep sea water in murine hepatoma Hepa 1clc7 cells. In addition GSH levels were increased $1.3{\sim}1.4$ fold in a hardness range of $100{\sim}1,000$. The deep sea water showed 20.3 and 35.0% inhibition of 12-O- tetradecanoylphorbol-13-a-cetate (TPA)-induced ODC activity at hardness 800 and 1,000, respectively. Therefore, deep sea water is worth further investigation with respect to cancer chemoprevention or therapy.

키워드

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피인용 문헌

  1. Suppression of cancer progression and metastasis in HT-29 human colorectal adenocarcinomas by deep sea water vol.18, pp.1, 2013, https://doi.org/10.1007/s12257-012-0543-y
  2. Effect of yogurt containing deep sea water on health-related serum parameters and intestinal microbiota in mice vol.98, pp.9, 2015, https://doi.org/10.3168/jds.2015-9492