Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Screening of Thrombin Inhibitor and its DPPH Radical Scavenging Activity from Wild Insects.

자생곤충으로부터 트롬빈 저해물질의 탐색 및 DPPH radical 소거활성

  • Ryu, Hee-Young (Dept. of Food & Nutrition, Andong National University) ;
  • Heo, Jin-Cheol (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Hwang, Jae-Sam (Division of Agrobiology, National Institute of Science & Technology, RDA) ;
  • Kang, Seok-Woo (Division of Agrobiology, National Institute of Science & Technology, RDA) ;
  • Yun, Chi-Young (Department of Biology, Daejeon University) ;
  • Lee, Sang-Han (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Sohn, Ho-Yong (Dept. of Food & Nutrition, Andong National University)
  • 류희영 (안동대학교 식품영양학과) ;
  • 허진철 (경북대학교 식품공학과) ;
  • 황재삼 (농업과학기술연구원 농업생물부) ;
  • 강석우 (농업과학기술연구원 농업생물부) ;
  • 윤치영 (대전대학교 생물학과) ;
  • 이상한 (경북대학교 식품공학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Published : 2008.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The in vitro thrombin inhibitory activities of 304 crude extracts from 76 kinds of korean wild insects were evaluated. Measurement of thrombin time showed that the DMSO extracts of Acrida cinerea cinerea (Thunberg), Anax parthenope julius Brauer, Eurydema rugosa Motschulsky, and Stethophyma magister (Rehn) and the water extracts of Dolycoris baccarum Linne, Lixus divaricatus Motschulsky, Metrioptera bonneti, Moechotypa diphysis (Pascoe), Nicrophorus concolor sp., and Tomapoderus ruficollis (Fabricius) had strong thrombin inhibitory activity. No prominent changes of activated partial thromboplastin time by treatment of the selected extracts suggested direct inhibition of thrombin activity by the insect extracts. DPPH scavenging activity of selected extracts showed that the extract of A. cinerea cinerea (Thunberg), D. baccarum Linne, L. divaricatus Motschulsky and N. concolor sp. has good antioxidant activity as well as antithombin activity. Our results suggested that some of korean wild insects could be developed as a natural source of antithrombosis.

국내의 자생곤충 76종으로부터 다양한 용매를 이용하여 304종의 추출물을 조제한 후, 이들의 항혈전 활성을 평가하였다. 먼저 트롬빈 저해활성 평가 결과, 방아깨비, 왕잠자리, 비단노린재 및 끝검은메뚜기의 DMSO 추출물 4종, 알락수염노린재, 가시길쭉바구미, 잔날개여치, 털두꺼비하늘소, 송장벌레과 유충 및 등빨간거위벌레의 물 추출물 6종에서 우수한 활성을 확인하였다. 선정된 10종을 대상으로 aPTT 측정결과 비단노린재, 방아깨비 및 잔날개여치 추출물에서 미약한 증가가 나타났으나, 전반적으로 혈전 생성 저해의 유의적인 변화는 인정되지 않아, 이들 추출물들은 트롬빈을 직접 저해하는 것으로 추측되었다. 한편 항트롬빈 활성이 우수한 10종 추출물 중에서 방아깨비, 가시길쭉바구미, 알락수염노린재 및 송장벌레과 유충 추출물에서는 우수한 DPPH 소거능을 나타내어 이들의 약용 곤충자원으로의 개발 가능성을 확인하였다.

Keywords

References

  1. Ahn, M. Y., B. S. Hahn, K. S. Ryu and S. I. Cho. 2002. Effect of insect crude drugs on blood coagulation and fibrinolysis system. Nat. Prod. Sci. 8, 66-70.
  2. Ahn, M. Y., K. S. Ryu, B. Y. Park, D. W. Kim, I. Kim and S. H. Kim. 2000. Effect of cricket on the chicken and its egg. Kor. J. Poult. Sci. 27, 197-202.
  3. Ahn, M. Y., Y. W. Lee, K. S. Ryu, H. S. Lee, I. Kim, J. W. Kim, Y. K. Lee, E. S. Kim and Y. S. Kim. 2002. Protective effects of water/methanol extracts of cricket on the acute hepatic damages in the ICR-mice induced by administration of $CCl_4$. Kor. J. Food Sci. Technol. 34, 684-687.
  4. Butenas, S. and K. G. Mann. 2002. Blood coagulation. Biochemisrty (Moscow) 67, 3-12.
  5. Hahn, B. S., S. G. Wu, S. W. Kim and Y. S. Kim. 1999. Evaluation of anticoagulant and fibrinolytic activities from crude extracts of insets. Kor. J. Pharmacogn. 30, 409-412.
  6. Han, S. M., S. H. Lee, C. Y. Yun, S. W. Kang, K. G. Lee, I. S. Kim, E. Y. Yun, P. J. Lee, S. Y. Kim and J. S. Hwang. 2006. Inhibition of nitric oxide production by ladybug extracts (Harmonia axyridis) in LPS-activated BV-2 cells. Kor. J. Appl. Entomol. 45, 31-36.
  7. Heo, J, C., J. Y. Park, J. S. Hwang, H. C. Park, S. W. Kang, S. J. Hwang, C. Y. Yun, T. K. Kwon and S. H. Lee. 2006. Comparison of in vitro antioxidant activity and cyclooxygenase- 2 promoter inhibitory activity in Harmonia axyridis Pallas and Coccinella septempunctata Linne. Kor. J. Food Preserv. 13, 513-518.
  8. Hsieh, K. H. 1997. Thrombin interaction with fibrin polymerization sites. Thrombosis Research 86, 301-316. https://doi.org/10.1016/S0049-3848(97)00073-X
  9. Korea National Statistical Office. 2000. A study on causes of death for 1999.
  10. Kwon, C. S., Y. S. Kwon, Y. S. Kim, G. S. Kwon, I. Jin, G. C. Ryu and H. Y. Sohn. 2004. Inhibitory activities of edible and medicinal herbs against human thrombin. J. Life Sci. 14, 509-513. https://doi.org/10.5352/JLS.2004.14.3.509
  11. Lee, S. S., S. M. Kim, U. Y. Park, H. Y. Kim and I. S. Shin. 2002. Studies on proteolytic and fibrinolytic activity of Bacillus subtilis JM-3 isolated from anchovy sauce. Kor. J. Food Sci. Technol. 34, 283-289.
  12. Morota, A., H. Isawa, Y. Orito, S. Iwanaqa, Y. Chinzei and M. Yuda. 2006. Identification and characterization of a collagen- induced platelet aggregation inhibitor, triplatin from salivary glands of the assassin bug. Triatoma infestans. FEBS J. 273, 2955-2962. https://doi.org/10.1111/j.1742-4658.2006.05306.x
  13. Noeske-Junqblut, C., J. Kratzschmar, B. Haendler, A. Alaqon, L. Possani, P. Verhallen, P. Donner and W. D. Schleuning. 1994. An inhibitor of collagen-induced platelet aggregation from the saliva of Triatoma pallidipennis. J. Biol. Chem. 269, 5050-5053.
  14. Park, D. S., M. A., Yoon, M. Z. Xu, H. Yu, J. R. Kim, T. S. Jeong and H. Y. Park. 2004. Screening of anti-atherogenic substances from insect resources. Kor. J. Pharmacogn. 35, 233-238.
  15. Park. J. Y., J. C. Heo, S. M. An, E. Y. Yun, S. M. Han, J. S. Hwang, S. W. Kang, C. Y. Yun and S. H. Lee. 2005. High throughput-compatible screening of anti-oxidative substances by insect extract library. Kor. J. Food Preserv. 12, 482-488.
  16. Ryu, H. Y., K. H. Bae, E. J. Kum, S. J. Park, B. H. Lee and H. Y. Sohn. 2007. Evaluation for the antimicrobial, antioxidant and antithrombosis activity of natural spices for fresh-cut yam. J. Life Sci. 17, 652-657. https://doi.org/10.5352/JLS.2007.17.5.652
  17. Ryu, H. Y., Y. K. Kim, I. S. Kwon, C. S. Kwon, I. Jin and H. Y. Sohn. 2007. Thrombin inhibition activity of fructus extract of Crataegus pinnatifida Bunge. J. Life Sci. 17, 535-539. https://doi.org/10.5352/JLS.2007.17.4.535
  18. Sohn, H. Y., C. S. Kwon, K. H. Son, G. S. Kwon, H. Y. Ryu and E. J. Kum. 2006. Antithrombin and thrombosis prevention activity of buckwheat seed, Fagopyrum esculentum Moench. J. Kor. Soc. Food Sci. Nutr. 35, 132-138. https://doi.org/10.3746/jkfn.2006.35.2.132
  19. Sohn, H. Y., C. S. Kwon, K. H. Son, G. S. Kwon, Y. S. Kwon, H. Y. Ryu and E. J. Kum. 2005. Antithrombosis and antioxidant activity of methanol extract from different brands of rice. J. Kor. Soc. Food Sci. Nutr. 34, 593-598. https://doi.org/10.3746/jkfn.2005.34.5.593
  20. Sohn, H. Y., Y. S. Kwon, Y. S. Kim, H. Y. Kwon, G. S. Kwon, G. J. Kim, C. S. Kwon and K. H. Son. 2004. Screening of thrombin inhibitors from medicinal and wild plants. Kor. J. Pharmacogn. 35, 52-61.
  21. Yun, E. Y., T. W. Goo, J. S. Hwang, S. H. Kim, S. W. Kang, K. Y. Kim and B. R. Jin. 2002. Characterization and expression of antibacterial protein gene, nuecin. Kor. J. Seric. Sci. 44, 64-68.
  22. Yun, Y. P., W. S. Kang and M. Y. Lee. 1996. The antithrombotic effects of green tea catechins. J. Fd Hyg. Safety 11, 77-82.
  23. Yun-Choi, H. S., K. S. Chung, M. H. Kim and J. H. Oh. 1995. Antithrombotic effects of some traditional plant medicines. Kor. J. Pharmacogn. 26, 154-158.

Cited by

  1. Present and Perspective on Insect Biotechnology vol.30, pp.6, 2015, https://doi.org/10.7841/ksbbj.2015.30.6.257
  2. Biological Activity and Chemical Characteristics of Fermented Silkworm Powder by Mold vol.20, pp.2, 2010, https://doi.org/10.5352/JLS.2010.20.2.237
  3. Improvement Effect of Fermented Silkworm (Bombyx mori L.) Powder against Orotic Acid-induced Fatty Liver in Rats vol.23, pp.6, 2013, https://doi.org/10.5352/JLS.2013.23.6.789
  4. Antioxidant and Antiatopic Effects of Refined Chaff Liquid Smoke vol.18, pp.2, 2011, https://doi.org/10.11002/kjfp.2011.18.2.266
  5. Comparison of fibrinolytic activity from Korean indigenous insects vol.51, pp.2, 2013, https://doi.org/10.7852/jses.2013.51.2.147
  6. Evaluation of Antioxidant and Nitrite Scavenging Activity of Seaweed Extracts vol.21, pp.4, 2011, https://doi.org/10.5352/JLS.2011.21.4.576