생명과학회지 (Journal of Life Science)

  • 제18권3호
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  • Pages.363-368
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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자생곤충으로부터 트롬빈 저해물질의 탐색 및 DPPH radical 소거활성

Screening of Thrombin Inhibitor and its DPPH Radical Scavenging Activity from Wild Insects.

  • 류희영 (안동대학교 식품영양학과) ;
  • 허진철 (경북대학교 식품공학과) ;
  • 황재삼 (농업과학기술연구원 농업생물부) ;
  • 강석우 (농업과학기술연구원 농업생물부) ;
  • 윤치영 (대전대학교 생물학과) ;
  • 이상한 (경북대학교 식품공학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Ryu, Hee-Young (Dept. of Food & Nutrition, Andong National University) ;
  • Heo, Jin-Cheol (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Hwang, Jae-Sam (Division of Agrobiology, National Institute of Science & Technology, RDA) ;
  • Kang, Seok-Woo (Division of Agrobiology, National Institute of Science & Technology, RDA) ;
  • Yun, Chi-Young (Department of Biology, Daejeon University) ;
  • Lee, Sang-Han (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Sohn, Ho-Yong (Dept. of Food & Nutrition, Andong National University)
  • 발행 : 2008.03.31
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초록

국내의 자생곤충 76종으로부터 다양한 용매를 이용하여 304종의 추출물을 조제한 후, 이들의 항혈전 활성을 평가하였다. 먼저 트롬빈 저해활성 평가 결과, 방아깨비, 왕잠자리, 비단노린재 및 끝검은메뚜기의 DMSO 추출물 4종, 알락수염노린재, 가시길쭉바구미, 잔날개여치, 털두꺼비하늘소, 송장벌레과 유충 및 등빨간거위벌레의 물 추출물 6종에서 우수한 활성을 확인하였다. 선정된 10종을 대상으로 aPTT 측정결과 비단노린재, 방아깨비 및 잔날개여치 추출물에서 미약한 증가가 나타났으나, 전반적으로 혈전 생성 저해의 유의적인 변화는 인정되지 않아, 이들 추출물들은 트롬빈을 직접 저해하는 것으로 추측되었다. 한편 항트롬빈 활성이 우수한 10종 추출물 중에서 방아깨비, 가시길쭉바구미, 알락수염노린재 및 송장벌레과 유충 추출물에서는 우수한 DPPH 소거능을 나타내어 이들의 약용 곤충자원으로의 개발 가능성을 확인하였다.

The in vitro thrombin inhibitory activities of 304 crude extracts from 76 kinds of korean wild insects were evaluated. Measurement of thrombin time showed that the DMSO extracts of Acrida cinerea cinerea (Thunberg), Anax parthenope julius Brauer, Eurydema rugosa Motschulsky, and Stethophyma magister (Rehn) and the water extracts of Dolycoris baccarum Linne, Lixus divaricatus Motschulsky, Metrioptera bonneti, Moechotypa diphysis (Pascoe), Nicrophorus concolor sp., and Tomapoderus ruficollis (Fabricius) had strong thrombin inhibitory activity. No prominent changes of activated partial thromboplastin time by treatment of the selected extracts suggested direct inhibition of thrombin activity by the insect extracts. DPPH scavenging activity of selected extracts showed that the extract of A. cinerea cinerea (Thunberg), D. baccarum Linne, L. divaricatus Motschulsky and N. concolor sp. has good antioxidant activity as well as antithombin activity. Our results suggested that some of korean wild insects could be developed as a natural source of antithrombosis.

키워드

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