생명과학회지 (Journal of Life Science)

  • 제18권3호
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  • Pages.323-328
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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효소 처리 홍삼을 함유한 오일의 항산화 효과

Antioxidant Effect of Oil Containing Cellulase-Treated Red Ginseng.

  • 김현정 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 양선아 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터) ;
  • 임남경 (계명대학교 식품가공학과) ;
  • 지광환 (금오공과대학교 자연과학부 응용화학) ;
  • 이인선 (계명대학교 전통 미생물자원 개발 및 산업화 연구센터)
  • Kim, Hyun-Jeong (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Yang, Seun-Ah (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Im, Nam-Kyung (Department of Food and Technology, Keimyung University) ;
  • Jhee, Kwang-Hwan (Dept. of Applied Chemistry, Kumoh National Institute of Technology) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 발행 : 2008.03.31
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초록

홍삼의 유효 활성 성분을 함유한 오일을 제조하고자, 세절한 홍삼을 여러 효소로 처리하여 항산화능을 검토한 후 가장 적합한 효소를 선정하여 홍삼을 효소처리하고 10배 비율의 대두유 및 올리브유를 각각 첨가하여 $40^{\circ}C$ 에서 15일간 숙성하면서 홍삼유를 제조하였다. 그 결과, 0.5% cellulase로 처리한 홍삼액에서 가장 높은 항산화 활성이 있음을 확인하였다. 그리고 홍삼을 0.5% cellulase로 처리하여 제조한 홍삼유의 과산화물가는 오일 자체만 보관한 대조군에 비해 유의적으로 감소되었고, 홍삼의 항산화 활성 성분의 용출은 올리브유보다 대두유에서 더 증가하였고, 특히 0.5% cellulase 처리한 홍삼 대두유의 TBA가도 유의적으로 감소되었다. 홍삼유의 DPPH radical 소거능도 홍삼만으로 추출한 대두유에 비해 0.5% cellulase 처리한 홍삼 대두유에서 더 큰 DPPH 소거능을 보였다. 대두유로 제조한 홍삼유의 항산화비타민 함량 분석에서는 비타민 A는 검출되지 않았으나, 비타민 E 함량은 대두유보다 홍삼 첨가 대두유에서 1.7배 정도 증가하였고, 0.5% cellulase를 처리한 홍삼첨가 대두유에서 2.3배 정도 증가되어 홍삼의 0.5% cellulase 처리에 의해 비타민 E의 용출이 더 증가하였다.

In this study we evaluated the method to develop red ginseng oil containing high content of phytochemicals by enzymes treatment. To select the optimum extraction process of red ginseng with oils, the antioxidant activities of red ginseng using various enzymes were measured. Red ginseng after 0.5% cellulase treatment for 1 hr at $50^{\circ}C$ had higher antioxidant activity than the other conditions. We found that red ginseng/soybean oil extracted for 15 days at $40^{\circ}C$ after 0.5% cellulase treatment increased DPPH radical scavenger activity and decreased the TBA and POV values. However, red ginseng/olive oil had little functional activities compare to the red ginseng/soybean 0il. We also analyzed vitamin A and E by HPLC and found that vitamin E was increased by 0.5% cellulase treatment in the oil. This is the first report that red ginseng oil extracted by enzyme treatment has various beneficial effects.

키워드

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피인용 문헌

  1. Effects of Extrusion and Enzyme Treatment on Extraction of β-Glucan from Agaricus blazei Murill vol.45, pp.3, 2016, https://doi.org/10.3746/jkfn.2016.45.3.380