YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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The Effect of Betulinic Acid on $TNF-{\alpha}-induced$ MCP-1 Expression in HL-60 Cells

HL-60 세포에서 $TNF-{\alpha}$에 의한 MCP-1 발현에 미치는 Betulinic Acid의 효과

  • Kim, Kyung-Chan (Department of Internal Medicine, College of Medicine, Catholic University of Daegu) ;
  • Lee, Chu-Hee (Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University)
  • 김경찬 (대구가톨릭대학교 의과대학) ;
  • 이추희 (영남대학교 의과대학)
  • Published : 2008.02.29
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Abstract

Betulinic acid, a naturally occurring pentacyclic triterpenoid, is found in abundance in the outer bark of white birch (Betula alba). In this study, we investigated if betulinic acid affects cytokine expression from activated macrophage cells. ELISA result showed that stimulation of HL-60 cells with proinflammatory cytokine such as $TNF-{\alpha}$ resulted in MCP-1 release into culture medium. In addition, transcriptional upregulation of MCP-1 in response to $TNF-{\alpha}$ was observed by RT-PCR analysis. However, incubation of HL-60 cells with betulinic acid prior to $TNF-{\alpha}$ treatment abrogated MCP-1 expression in transcription and translational level. Consistent with a number of studies which reported requirement of ERK activation for $TNF-{\alpha}$ expression, Western blot analysis showed that $TNF-{\alpha}-induced$ ERK activation was suppressed by pretreatment of HL-60 cells with betulinic acid. Taken together, our data indicate that betulinic acid exerts its anti-inflammatory effect through inhibition of $TNF-{\alpha}-induced$ ERK activation which is required for the subsequent MCP-1 release.

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References

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