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Utilization of Probiotic Starter Cultures for the Manufacture of Low-fat Functional Fermented Sausages

저지방 기능성 발효소시지의 제조를 위한 복합 유산균주의 이용

  • Kim, Young-J. (Department of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Lee, Hong-C. (Department of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Park, Sung-Y. (Department of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Park, Sun-Y. (Department of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Oh, Se-Jong (Department of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Chin, Koo-B. (Department of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University)
  • 김영주 (전남대학교 동물자원학부 및 농업과학기술연구소) ;
  • 이홍철 (전남대학교 동물자원학부 및 농업과학기술연구소) ;
  • 박성용 (전남대학교 동물자원학부 및 농업과학기술연구소) ;
  • 박선영 (전남대학교 동물자원학부 및 농업과학기술연구소) ;
  • 오세종 (전남대학교 동물자원학부 및 농업과학기술연구소) ;
  • 진구복 (전남대학교 동물자원학부 및 농업과학기술연구소)
  • Published : 2008.03.30

Abstract

This study was performed to evaluate the physico-chemical properties of fermented sausages containing probiotic starter cultures (LK-30 plus, Lactobacillus plantarum 155 and 167, and Pediococcus damnosus L12) with reduced fat levels, and to determine the optimum condition for the manufacture of these products. Although low-fat fermented sausages were reduced fat content at the amount of 90% and the ripening time by 1-2 weeks, as compared to regular-fat counterpart, they became harder and had many winkles outside due to the extreme drying. In addition, fat level in fermented sausages affected the composition and shear force values. During ripening, pH, lightness and yellowness values tended to decrease, however, microbial counts of inoculated lactic acid bacteria were increased up to $10^8-10^9cfu/g$ within 3 days and remained constant thereafter. Low-fat fermented sausages had higher microbial counts than regular-fat ones. Although the inoculated probiotic starter cultures alone had the functional properties, such as cholesterol reduction, anti-high blood pressure and antimicrobial activity, they did not have distinctive characteristics in the fermented sausages. Based on these results, the low-fat fermented sausages were successfully manufactured, but a little bit increased fat level and improved functional properties in the fermented sausages would be required to have better quality as compared to regular-fat counterparts.

본 연구는 지방을 비교적 많이 함유하는 발효소시지의 지방을 감소시키기 위해 대두단백질을 지방대체제로 이용하여 발효소시지를 제조하였으며, starter culture로는 상업화된 유산균주인 LK30 plus 균주를 대조구로 하여 콜레스테롤 저하와 항고혈압활성 및 S. aureus에 대한 항균력을 갖는 기능성 유산균주를 복합 starter culture로 접종하여 숙성기간동안 제품의 이화학적, 조직학적, 미생물적 특성과 콜레스테롤 저하 및 항고혈압 활성과 S. aureus의 균수의 변화를 통해 항균활성을 평가하였다. 지방을 첨가하지 않는 저지방 발효소시지는 기존의 고지방 발효소시지에 비해 약 90% 이상의 지방을 감소시켰고, 숙성시간을 약 7-14일 정도 단축시킬 수 있었나, 저지방 발효소시지에서 나타나는 지나친 감량에 의한 외관의 주름 및 경도의 증가가 나타났다. 발효소시지들의 일반성분 검사 결과, 숙성시간이 경과됨에 따라 수분은 감소하는데 반해, 지방과 단백질의 함량은 상대적으로 증가하였으며, 제품의 pH는 발효기간 동안 감소되어졌다가 숙성기간동안 유지되는 경향을 보였다. 또한, 숙성 중 명도는 감소하고, 전단력과 무게감량은 증가하는 경향을 보였다. 콜레스테롤 저하와 항고혈압 활성 및 S. aureus에 대한 항균활성과 같은 기능성은 발효소시지내에서는 뚜렷한 효과를 보이지 않았다. '따라서 저지방 발효소시지에서 나타나는 과다한 주름을 방지하기 위하여 일정함량의 지방첨가와 probiotic 유산균 주가 함유하는 기능성이 발효 육제품에도 나타날 수 있도록 하는 보다 심도 있는 연구가 필요할 것으로 판단된다.

Keywords

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