한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제23권1호
  • /
  • Pages.25-30
  • /
  • 2008
  • /
  • 2508-755X(pISSN)
  • /
  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지

한우의 임신 초기 혈장단백질 발현 양상 분석

Proteomic Analysis of Plasma Protein during Early Pregnancy in Korean Native Cattle (Hanwoo)

  • 김평희 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 배성훈 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 오석두 (국립진주산업대학교 동물생명공학과) ;
  • 고응규 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 양병철 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 김명직 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 진동일 (충남대학교 축산학과) ;
  • 성환후 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 황성수 (농촌진흥청 축산과학원 응용생명공학과)
  • 발행 : 2008.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study was performed to identify and analyze the specifically expressed plasma proteins during early pregnancy in both pregnant and non-pregnant Hanwoo. Blood samples were collected at 0 (the day of AI), 2, 3, 4, 7, and 11 weeks after AI from pregnant (n=3) and non-pregnant (n=4) Hanwoo, respectively. The hematological parameters were measured. After 2-dimensional electrophoresis using serum, normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups. Among 17 spots selected, 15 were identified as albumin, IgG1 heavy chain constant region, haptoglobin, ferrochelatase, fibrinogen, hemopexin. 5 spots were expressed only in non-pregnant specific. The spot identification of 1105 and 6106 was decreased after 3 weeks from AI. However, 2/17 spots were still unidentified. Further studies are needed to analyze the function of the proteins associated with early pregnancy.

키워드

참고문헌

  1. Ahmed N, Barker G, Oliva K, Garfin D, Talmadge K, Georgiou H, Quinn M and Rice G. 2003. An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum. Proteomics 3:1980-1987 https://doi.org/10.1002/pmic.200300465
  2. An JY, Fan ZM, Zhuang ZH, Qin YR, Gao SS, Li JL and Wang LD. 2004. Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province. World J. Gastroenterol. 10:3365-3368 https://doi.org/10.3748/wjg.v10.i22.3365
  3. Bloomer JR, Morton KO, Reuter RJ and Ruth GR. 1982. Bovine protoporphyria: Documentation of autosomal recessive inheritance and comparison with the human disease through measurement of heme synthase activity. Am. J. Hum. Genet. 34:322-330
  4. Burtist CA and Ashwood ER. 1994. Tietz Textbook of Clinical Chemistry. 2nd ed. WB Saunders Company. Philadelphia, pp. 688-691
  5. Fu Q, Garnham CP, Elliott ST, Bovenkamp DE and Van Eyk JE. 2005. A robust, streamlined, and reproducible method for proteomic analysis of serum by delipidation, albumin and IgG depletion, and two-dimensional gel electrophoresis. Proteomics 5:2656-2664 https://doi.org/10.1002/pmic.200402048
  6. Govorukhina NI, Keizer-Gunnink A, van der Zee AG, de Jong S, de Bruijn HW and Bischoff R. 2003. Sample preparation of human serum for the analysis of tumor markers. Comparison of different approaches for albumin and gamma-globulin depletion. J. Chromatogr. A. 1009:171-178 https://doi.org/10.1016/S0021-9673(03)00921-X
  7. Guillomot M. 1995. Cellular interactions during implantation in domestic ruminants. J. Reprod. Fertil. Suppl. 49:39-51
  8. Hoffer-Goeres KA, Batchelder CA, Bertolini M, Moyer AL, Famula TR and Anderson GB. 2007. Angiogenesis in day- 30 bovine pregnancies derived from nuclear transfer. Cloning and Stem Cells 9:595-607 https://doi.org/10.1089/clo.2007.0019
  9. Kim HR, Kang JK, Yoon JT, Seong HH, Jung JK, Lee HM, Park CS and Jin DI. 2005. Protein profiles of bovine placenta derived from somatic cell nuclear transfer. Proteomics 5:4264-4273 https://doi.org/10.1002/pmic.200401297
  10. Kim YS, Kim MS, Lee SH, Choi BC, Lim JM, Cha KY and Baek KH. 2006. Proteomic analysis of recurrent spontaneous abortion: Identification of an inadequately expressed set of proteins in human follicular fluid. Proteomics 6: 3445-3454 https://doi.org/10.1002/pmic.200500775
  11. King GJ, Atkinson BA and Robertson HA. 1979. Development of the bovine placentome during the second month of gestation. J. Reprod. Fertil. 55:173-180 https://doi.org/10.1530/jrf.0.0550173
  12. Lee SY, Park JY, Choi YJ, Cho SK, Ahn JD, Kwon DN, Hwang KC, Kang SJ, Paik SS, Seo HG, Lee HT and Kim JH. 2007. Comparative proteomic analysis associated with term placental insufficiency in cloned pig. Proteomics 7: 1303-1315 https://doi.org/10.1002/pmic.200601045
  13. Oakley BR, Kirsch DR and Morris NR. 1980. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal. Biochem. 105:361-363 https://doi.org/10.1016/0003-2697(80)90470-4
  14. Riding GA, Jones A, Holland MK, Hill JR and Lehnert SA. 2008. Proteomic analysis of bovine conceptus fluids during early pregnancy. Proteomics 8:160-177 https://doi.org/10.1002/pmic.200700465
  15. Ruth GR, Schwartz S and Stephenson B. 1977. Bovine protoporphyria: the first non-human model of this hereditary photosensitizing disease. Science 198:199-201 https://doi.org/10.1126/science.905823
  16. Shibuya H, Nonneman D, Tamassia M, Allphin OL and Johnson GS. 1995. The coding sequence of the bovine ferroche latase gene. Biochim. Biophys. Acta. 1231:117-120 https://doi.org/10.1016/0005-2728(95)00077-V
  17. Steel LF, Trotter MG, Nakajima PZ, Mattu TS, Gonye G and Block T. 2003. Efficient and specific removal of albumin from human serum samples. Mol. Cell. Proteomics 2:262- 270 https://doi.org/10.1074/mcp.M300026-MCP200
  18. Straka JG, Hill HD, Krikava JM, Kools AM and Bloomer JR. 1991. Immunochemical studies of ferrochelatase protein: characterization of the normal and mutant protein in bovine and human protoporphyria. Am. J. Hum. Genet. 48:72-78
  19. Taponen J, Katila T and Rodriguez-Martinez H. 1999. Induction of ovulation with gonadotropinreleasing hormone during proestrus in cattle: Influence on subsequent follicular growth and luteal function. Anim. Reprod. Sci. 55:91-105 https://doi.org/10.1016/S0378-4320(99)00011-1
  20. Todd DJ. 1994. Erythropoietic protoporphyria. Br. J. Dermatol. 131:751-766 https://doi.org/10.1111/j.1365-2133.1994.tb08577.x
  21. Turzillo AM and Fortune JE. 1990. Suppression of the secondary FSH surge with bovine follicular fluid is associated with delayed ovarian follicular development in heifers. J. Reprod. Fertil. 89:643-653 https://doi.org/10.1530/jrf.0.0890643
  22. Wang YY, Cheng P and Chan DW. 2003. A simple affinity spin tube filter method for removing high-abundant common proteins or enriching low-abundant biomarkers for serum proteomic analysis. Proteomics 3:243-248 https://doi.org/10.1002/pmic.200390036
  23. Wassell J. 2000. Haptoglobin: Function and polymorphism. Clin. Lab. 46:547-552
  24. 김영훈, 고진철, 오창언, 강승률, 양보석, 오성종, 김창능, 송중용, 김일화. 2006. CIDR를 이용한 제주 한우 및 흑우의 체내 수정란 생산과 이식. 한국수정란이식학회지 21:191-198
  25. 성환후, 양병철, 황성수, 박성재, 고응규, 노환국, 양보석. 2006. Anti-inhibin serum 및 bovine follicular fluid 처리가 한우 난소의 난포 발달과 혈중 호르몬 변화에 미치는 영향. 한국수정란이식학회지 21:315-322
  26. 이귀녕, 이종순. 1996. 임상병리화일. 의학문화사. 서울, pp. 192-194