Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Heme Oxygenase-1 (HO-1) Induction by UVB (290-320nm) Radiation in ICR Mice

ICR mouse의 피부와 간에서 자외선 B파에 의한 Heme Oxygenase-1의 유도

  • Choi, Wook-Hee (Department of Health Science, Dongduk Women's University) ;
  • Kim, Tae-Hwan (College of Veterinary Medicine, Kyungbook National University) ;
  • Ahn, Ryoung-Me (Department of Health Science, Dongduk Women's University)
  • 최욱희 (동덕여자대학교 보건관리학과) ;
  • 김태환 (경북대학교 수의학과) ;
  • 안령미 (동덕여자대학교 보건관리학과)
  • Published : 2008.02.28
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Abstract

The induction of heme oxygenase-1(HO-1) by UV radiation provides a protective defence against oxidative stress, and has been well demonstrated in skin irradiated with UVA, but not UVB. In this study, we show that the induction of cutaneous HO-l can be attributed to UVB radiation. The expression of HO-1 mRNA was assessed in vivo by reverse transcription-polymerase chain reaction (RT-PCR) analysis, and HO-1 enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 1d to 3d after UVB $(3KJ/m^2)$ exposure. The results of gene expression were same pattern of HO-1 enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 4d after UVB exposure, but HO-1 activity in liver increased to 2d. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissue directly or indirectly from oxidative stress.

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References

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