Imaging Science in Dentistry
- 제38권1호
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- Pages.39-47
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- 2008
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- 2233-7822(pISSN)
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- 2233-7830(eISSN)
방사선조사가 Porphyromonas gingivalis에 미치는 영향
Effect of irradiation on the Porphyromonas gingivalis
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이창환
(경희대학교 치의학전문대학 구강악안면방사선학교실 및 구강생물학연구소) ;
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김규태
(경희대학교 치의학전문대학 구강악안면방사선학교실 및 구강생물학연구소) ;
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최용석
(경희대학교 치의학전문대학 구강악안면방사선학교실 및 구강생물학연구소) ;
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황의환
(경희대학교 치의학전문대학 구강악안면방사선학교실 및 구강생물학연구소)
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Lee, Chang-Hwan
(Department of Oral and Maxillofacial Radiology, School of Dentistry and Institute of Oral Biology, Kyung Hee University) ;
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Kim, Gyu-Tae
(Department of Oral and Maxillofacial Radiology, School of Dentistry and Institute of Oral Biology, Kyung Hee University) ;
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Choi, Yong-Suk
(Department of Oral and Maxillofacial Radiology, School of Dentistry and Institute of Oral Biology, Kyung Hee University) ;
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Hwang, Eui-Hwan
(Department of Oral and Maxillofacial Radiology, School of Dentistry and Institute of Oral Biology, Kyung Hee University)
- 발행 : 2008.03.31
초록
Purpose: The aim of this study was to observe a direct effect of irradiation on the periodontopathic Porphyromonas gingivalis (P. gingivalis). Materials and Methods: P. gingivalis 2561 was exposed to irradiation with a single absorbed dose of 10, 20, 30, and 40Gy. Changes in viability and antibiotic sensitivity, morphology, transcription, and protein profile of the bacterium after irradiation were examined by pour plating method, disc diffusion method, transmission electron microscopy, RT-PCR, and immunoblot, respectively. Results: Viability of irradiated P. gingivalis drastically reduced as irradiation dose was increased. Irradiated P. gingivalis was found to have become more sensitive to antibiotics as radiation dose was increased. With observation under the transmission electron microscope, the number of morphologically abnormal cells was increased with increasing of irradiation dose. In RT-PCR, decrease in the expression of fimA and sod was observed in irradiated P. gingivalis. In immunoblot, change of profile in irradiated P. gingivalis was found in a number of proteins including 43-kDa fimbrillin. Conclusion: These results suggest that irradiation may affect the cell integrity of P. gingivalis, which is manifested by the change in cell morphology and antibiotic sensitivity, affecting viability of the bacterium.