Journal of Crop Science and Biotechnology

The Korean Society of Crop Science (한국작물학회)
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A High-Efficiency Direct Somatic Embryogenesis System for Strawberry(Fragaria x ananassa Duch.) Cultivar Chandler

  • Husaini, Amjad M. (Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science) ;
  • Aquil, Samina (Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science) ;
  • Bhat, Mukhtar (Department of Botany, Faculty of Science) ;
  • Qadri, Tabassum (Department of Botany, Faculty of Science) ;
  • Kamaluddin, Kamaluddin (Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science) ;
  • Abdin, Malik Zainul (Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science)
  • Published : 2008.06.30
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Abstract

A high-efficiency, reproducible somatic embryogenesis system for strawberry cultivar Chandler was developed. Thirty-one somatic embryos per explant(max no.) were recorded in leaf discs which were cultured on medium containing MS salts+$B_5$ vitamins+2% glucose+4.0 mg $1^{-1}$TDZ(Thidiazuron) and incubated at $10{\pm}1^{\circ}C$ under darkness for one week followed by three weeks under 16-h photoperiod. The scanning electron microscopic(SEM) ontogeny revealed the normal development of somatic embryos from globular to heart-shaped and dissection microscopy from torpedo-shaped to cotyledonary-stage embryos. The maximum germination percentage of 48% could be obtained on MS medium containing kinetin(1.0 mg $1^{-1}$) and the maximum survival percentage(79%) of plantlets after four weeks was found to be in the mixture of vermiculite, peatmoss, and soilrite(1:1:1).

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