Journal of Crop Science and Biotechnology

The Korean Society of Crop Science (한국작물학회)
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Suppressive Effects of Coumarins on Pumpkin Seedling Growth and Glutathione S-Transferase Activity

  • Hossain, Md. Daud (Bangladesh Agricultural Research Institute) ;
  • Li, Jing (College of Horticulture, Nanjing Agricultural University) ;
  • Guo, Shirong (College of Horticulture, Nanjing Agricultural University) ;
  • Fujita, Masayuki (Department of Plant Sciences, Faculty of Agriculture, Kagawa University)
  • Published : 2008.09.30
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Abstract

The effects of some coumarins(coumarin, 7-hydroxycoumarin, scopoletin and esculetin) were investigated on pumpkin(Cucurbita maxima Duch.) seedlings and on pumpkin glutathione S-transferases(GSTs). Coumarin and esculetin suppressed the growth of seedlings, especially the elongation of roots as well as hypocotyls. Among the compounds tested, only esculetin inhibited the activity of a particular pumpkin GST by 50%, CmGSTU3 toward 1-chloro-2, 4- dinitrobenzene(CDNB) and at a concentration of 22 ${\mu}M$. Both ethylacetae(EtOAc) and water fractions in pumpkin seedlings and different organs of one-month-old pumpkin plants contained esculetin or similar hydrophobic fluorescent substances as well as hydrophilic substances, which showed different degrees of inhibitory effects on CmGSTU3 activity.

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