Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Neuroprotective Effects of Berberine in Neurodegeneration Model Rats Induced by Ibotenic Acid

  • Lim, Jung-Su (Department of Life and Nanopharmaceutical Science, Kyunghee University) ;
  • Kim, Hyo-Sup (Department of Biology, Kyunghee University) ;
  • Choi, Yoon-Seok (Department of Biology, Kyunghee University) ;
  • Kwon, Hyock-Man (Department of Bioscience and Biotechnology & Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies) ;
  • Shin, Ki-Soon (Department of Life and Nanopharmaceutical Science, Kyunghee University) ;
  • Joung, In-Sil (Department of Biological Sciences, Hanseo University) ;
  • Shin, Mi-Jung (REGEN Biotech Inc.) ;
  • Kim, Yun-Hee (Department of Life and Nanopharmaceutical Science, Kyunghee University)
  • Published : 2008.12.31
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Abstract

Berberine, an isoquinoline alkaloid found in Coptidis Rhizoma(goldenthread) extract, has multiple pharmacological effects such as anti-inflammatory, antimicrobial and anti-ischemic effects. In the present study, we examined the effects of berberine on neuronal survival and differentiation in a hippocampal precursor cell line and in the memory deficient rat model. Berberine increased in a dose dependent manner the survival of hippocampal precursor cells as well as differentiated cells. In addition, berberine promoted neuronal differentiation of hippocampal precursor cells. In the memory deficient rat model induced by stereotaxic injection of ibotenic acid into entorhinal cortex(Ibo model), hippocampal cells were increased about 2.7 fold in the pyramidal layer of CA1 region and about 2 fold in the dentate gyrus by administration of berberine after 2 weeks of ibotenic acid injection. Furthermore, neuronal cells immunoreactive to calbindin were increased in the hippocampus and entorhinal cortex area by administration of berberine. Taken together, these results suggest that berberine has neuroprotective effect in the Ibo model rat brain by promoting the neuronal survival and differentiation.

Keywords

References

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