Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Practical Use of DNA Polymorphisms in the Avian Immunoglobulin Light Chain Constant Domain for Species-specific PCR

조류의 종 특이 구별을 위한 항체 유전자의 이용

  • Choi, J.W. (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kang, S.J. (Department of Agricultural Biotechnology, Seoul National University) ;
  • Park, M.S. (Department of Microbiology, College of Natural Sciences, Changwon National University) ;
  • Kim, J.-K. (Department of Microbiology, College of Natural Sciences, Changwon National University) ;
  • Han, J.Y. (Department of Agricultural Biotechnology, Seoul National University)
  • 최진원 (서울대학교 농생명공학부) ;
  • 강석진 (서울대학교 농생명공학부) ;
  • 박명선 (창원대학교 미생물학과) ;
  • 김진규 (창원대학교 미생물학과) ;
  • 한재용 (서울대학교 농생명공학부)
  • Published : 2008.02.01
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Abstract

Species-specific polymorphisms in chicken, pheasant, turkey, and quail were identified by cloning and sequencing of the immunoglobulin constant domain (IgLC). A set of species-specific primers were then designed on the basis of polymorphisms in the IgLC between species, as well as two additional sets of primers for the cytochrome b and tapasin genes, for the purpose of species identification. Together, the primers successfully distinguished specific species from chicken by species-specific PCR. This simple but unambiguous method may be used to screen avian inter-species germline chimeras, which are valuable models for the conservation of endangered species.

본 연구에서는 조류에서 종 특이적인 DNA 염기서열변이를 검증하기 위하여 닭, 꿩, 칠면조, 메추리의 immunoglobulin light chain constant domain 유전자를 클로닝하여 DNA염기서열을 분석하였다. 종간에 구별이 가능한 DNA 염기서열변이가 위의 유전자에서 관찰되었다. PCR을 이용하여 종을 구별하기 위하여 종 사이에 특이적인 DNA 염기서열 부위에 한 쌍의 종 특이적인 프라이머를 제작하였다. 또한 비교실험을 위하여 이미 알려진 cytochrome b와 tapasin 유전자에서도 두 쌍의 종 특이적 프라이머를 제작하였다. PCR결과 세 쌍의 프라이머 모두 종 특이적으로 DNA를 증폭하였다. Immunoglobulin 유전자의 염기서열 변이를 이용한 종 특이적인 PCR 방법은 조류의 유전자원 보존을 위한 이종간 카이메라 연구에 유용하게 이용될 수 있을 것이다.

Keywords

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