Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Determination of saikosaponin derivatives in Bupleuri Radix using HPLC-ELSD

HPLC-ELSD를 이용한 시호 중의 saikosaponin 유도체의 확인법 개발

  • Published : 2008.02.21
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Abstract

A HPLC-ELSD method was developed to determine saikosaponin derivatives from Bupleuri Radix. Eight saikosaponins, saikosaponin c, i, h, a, $b_2$, g, $b_1$ and d, were analyzed under optimized HPLC conditions [column: Eclipse XDB $C_{18}$ ($150{\times}4.6mm$ i.d., $5{\mu}m$; mobile phase: $H_2O$ with 0.1% $CH_3$COOH (v/v) for solvent A and AcCN with 0.1% $CH_3$COOH (v/v) for solvent B, gradient elution; flow rate: 1mL/min; injection volume: $20{\mu}L$]. Good linearity was achieved in the range from 62.5 to $250{\mu}g/mL$ for each compound, and intra-day precision and accuracy at each concentration level varied between 0.05 and 5.45% and between 93.9 and 109.6%, respectively, whereas those for inter-day variations were between 0.91 to 2.73% and 94.3 to 106.1%. This HPLC-ELSD method was applied for the determination of sakosaponins from Bupleuri Radix samples, and saikosaponin a $(0.79{\pm}0.20mg/g)$, c $(0.33{\pm}0.06mg/g)$ and d $(0.48{\pm}0.15mg/g)$ were observed as major compounds. The other saikosaponins were shown under limit of quantification level thus couldn't be quantified. The present study suggested that the introduced HPLC-ELSD method is selective and reliable, and not only saikosaponin a, but also saikosaponin c and d should be employed as the standard markers for Bupleuri Radix.

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References

  1. K. Bae, The Medicinal Plants of Korea, Kyo-Hak, Seoul, Korea, p. 372 (1999)
  2. N. Ebata, H. Nakajima, K. Hayashi, M. Okada and M. Maruno, Saponins from the root of Bupleurum falcatum. Phytochemistry, 41, 895-901 (1996) https://doi.org/10.1016/0031-9422(95)00720-2
  3. L. Pistelli, A. Cammilli, A. Manunta, A. Marsili and I. Morelli, Triterpenoid saponins and flavonoid glycoside from Bupleurum falcatum subsp. cernuum. Phytochemistry, 33, 1537-1359 (1993) https://doi.org/10.1016/0031-9422(93)85130-J
  4. H. Ishii, S. Seo, K. Tori, T. Tozyo and Y. Yoshimura, The structure of saikosaponin-E and acetylsaikosaponins, minor components isolated from Bupleurum falcatum L., determined by C-13 NMR spectroscopy. Tetrahedron Lett., 18, 1227-1230 (1977) https://doi.org/10.1016/S0040-4039(01)92982-X
  5. H. Kohno, M. Yamazaki, N. Jyumonji, N. Yamaguchi and S. Odashima, An investigation of the immune suppression induced by some saikosaponin derivatives. Int. J. Immunopharmacol., 10, 845-850 (1998). https://doi.org/10.1016/0192-0561(88)90008-2
  6. N. Yamaguchi, H. Kohno, M. Tawara and S. Odashima, Effect of saikosaponin derivatives upon the immune response against T-dependent and T-independent antigens in mice. Int. J. Immunopharmacol., 7, 827-832 (1985) https://doi.org/10.1016/0192-0561(85)90044-X
  7. P. Bermejo Benito, M.J. Abad Martnez, A.M. Silvn Sen, A. Sanz Gmez, L. Fernndez Matellano, S. Snchez Contreras and A.M. Diaz Lanza, In vivo and in vitro antiinflammatory activity of saikosaponins. Life Sci., 63, 1147-1156 (1998) https://doi.org/10.1016/S0024-3205(98)00376-2
  8. W.S. Wu and H.Y. Hsu, Involvement of p-51INK4band p- 16INK4a gene expression in saikosaponin a and TPAinduced growth inhibition of HepG2 cells. Biochem. Biophys. Res. Commun., 285, 183-187 (2001) https://doi.org/10.1006/bbrc.2001.5152
  9. Y.L. Hsu, P.L. Kuo and C.C. Lin, The proliferative inhibition and apoptotic mechanism of Saikosaponin D in human non-small cell lung cancer A549 cells. Life Sci., 75, 1231-1242 (2004) https://doi.org/10.1016/j.lfs.2004.03.008
  10. J.C. Chen, N.W. Chang, J.G. Chung and K.C. Chen, Saikosaponin-A induces apoptotic mechanism in human breast MDA-MB-231 and MCF-7 cancer cells. Am. J. Chin. Med., 31, 363-377 (2003) https://doi.org/10.1142/S0192415X03001065
  11. L.C. Chiang, L.T. Ng, L.T. Liu, D.E. Shieh and C.C. Lin, Cytotoxicity and anti-hepatitis B virus activities of saikosaponins from Bupleurum species. Planta Med., 69, 705-709 (2003) https://doi.org/10.1055/s-2003-42797
  12. 대한약정 8개정 의약품 각조 제 2부
  13. B.C. Liau, S.S. Hsiao, M.R. Lee, T.T. Jong and S.T. Chiang, Quality control of Chinese medicinal preparations LC/ ESI(+)/MS/MS analyses of saikosaponin-a and -c as markers of Bupleuri redix samples. J. Pharm. Biomed. Anal., 43, 1174-1178 (2007) https://doi.org/10.1016/j.jpba.2006.10.002
  14. L. Liu, Y. Cheng and H. Zhang, Phytochemical analysis of anti-athrogenic constituents of Xue-Fu-Zhu-Yu-Tang using HPLC-DAD-ESI-MS. Chem. Pharm. Bull., 52, 1295-1301 (2004) https://doi.org/10.1248/cpb.52.1295
  15. H. Ishii, M. Nakamura, S. Seo, K. Tori, T. Tozyo and Y. Yoshimura, Isolation, characterization, and nuclear magnetic resonance spectra of new saponins from the roots of Bupleurum falcatum L. Chem. Pharm. Bull., 28, 2367-2383 (1980) https://doi.org/10.1248/cpb.28.2367
  16. K. Tori, S. Seo, Y. Yoshimura, M. Nakamura, T. Yutaka and H. Ishii, Carbon -13 NMR spectra of saikosaponin A, C, D and F isolated from Bupleurum falcatum L. Tetrahedron Lett., 46, 4167-4170 (1976)
  17. T. Kuboata and H. Hinoh, The constitution of saponins isolated from Bupleurum falcatum L. Tetrahedron Lett., 9, 303-306 (1967)
  18. A. Shimaoka, S. Seo and H. Minato, Saponins isolated from Bupleurum falcatum L.; Components of saikosaponin-B. J. Chem. Soc. Perkin Trans. I, 2043-2048a (1975)