Advances in Traditional Medicine

  • 제7권5호
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  • Pages.509-517
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  • 2008
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  • 2662-4052(pISSN)
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  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
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Effects of Gyejijakyakjimo-tang extract on inhibition of PGE2 synthesis and NO production in murine raw 264.7 macrophage cells

  • Park, Kyoung-Su (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University) ;
  • Hwang, Mi-Ja (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University) ;
  • Nam, Ki-Bong (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University) ;
  • Ryu, Ji-Mi (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University) ;
  • Chung, Seok-Hee (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University)
  • 발행 : 2008.02.29
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초록

Gyejijakyakjimo-tang is a multi-herbal formula that is composed of nine medicinal herbs. Gyejijakyakjimo-tang has been reported to have antipyretic and analgesic effects. Gyejijakyakjimo-tang has traditionally been used for goat and rheumatoid arthritis. However, analgesic and antiinflammatory effects of Gyejijakyakjimo-tang has not been clarified yet. In this study, we investigated the analgesic and anti-inflammatory effect of the aqueous extract of Gyejijakyakjimo-tang. We evaluated the aqueous extract of Gyejijakyakjimo-tang on Lipopolysaccharide (LPS)-induced inflammation in murine raw 264.7 macrophage cells. For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), prostaglandin $E_2$ ($PGE_2$) immunoassay, and nitric oxide (NO) detection were performed. Gyejijakyakjimo-tang suppressed $PGE_2$ synthesis and NO production by inhibiting the LPS-induced expressions of COX-2 and iNOS mRNA in murine raw 264.7 macrophage cells. These results show that Gyejijakyakjimo-tang has the analgesic and anti-inflammatory effect by mostly suppressing COX-2 and iNOS expressions, and resulting in the inhibition of $PGE_2$ synthesis and NO production.

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