Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Seroprevalence of Toxocariasis among Healthy People with Eosinophilia

  • Kim, Yong-Hun (Department of Parasitology, College of Medicine, Hallym University) ;
  • Huh, Sun (Department of Parasitology, College of Medicine, Hallym University) ;
  • Chung, Young-Bae (Department of Parasitology, College of Medicine, Jeju National University)
  • Published : 2008.03.31
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Abstract

The aim of this study is to determine the Toxocara seropositive rate among healthy people with eosinophilia. A total of 97 people residing in Seoul who were healthy and whose blood eosinophilia was over 10%, as shown by regular health check-ups in 2004, were subjected to this study. Their sera were tested by immunoblotting and ELISA with the antigen of larval Toxocara canis excretory-secretory (ES) protein. Sixty-five sera were band-positive (67.0%). The seropositve control sera were positive to band sizes of 66 kDa, 56 kDa, 32 kDa, and 13 kDa. In ELISA, 63 sera (65.0%) were positive to T. canis ES protein. There was no significant correlation between the IgG ELISA titer and the level of eosinophilia (r = 0.156, P = 0.156). As there were insufficient data to determine whether there were cross-reactions with other helminthic infections, or whether atopy occurred, further studies are required to verify the cause of the seropositive reactions against T. canis ES antigen. Toxocariasis seropositivity is suggested to be the major cause of eosinophilia, since the Toxocara seroprevalence among Korean rural adults was shown to be approximately 5%.

Keywords

References

  1. Magnaval JF, Michault A, Calon N, Charlet JP. Epidemiology of human toxocariasis in La Reunion. Trans R Soc Trop Med Hyg 1994; 88: 531-533 https://doi.org/10.1016/0035-9203(94)90148-1
  2. Kim YH, Huh S. Prevalence of Toxocara canis, Toxascaris leonina and Dirofilaria immitis in dogs in Chuncheon, Korea (2004). Korean J Parasitol 2005; 43: 65-67 https://doi.org/10.3347/kjp.2005.43.2.65
  3. Park HY, Lee SU, Huh S, Kong Y, Magnaval JF. A seroepidemiological survey for toxocariasis in apparently healthy residents in Gangwon-do, Korea. Korean J Parasitol 2002; 40: 113-117 https://doi.org/10.3347/kjp.2002.40.3.113
  4. Malla N, Aggarwal AK, Mahajan RC. A serological study of human toxocariasis in north India. Natl Med J India 2002; 15: 145-147
  5. Logar J, Soba B, Kraut A, Stirn-Kranjc B. Seroprevalence of Toxocara antibodies among patients suspected of ocular toxocariasis in Slovenia. Korean J Parasitol 2004; 42: 137-140 https://doi.org/10.3347/kjp.2004.42.3.137
  6. Fillaux J, Santillan G, Magnaval JF, Jensen O, Larrieu E, Sobrino-Becaria CD. Epidemiology of toxocariasis in a steppe environment: the Patagonia study. Am J Trop Med Hyg 2007; 76: 1144-1147
  7. Choi JH, Suh YJ, Jung JW, Song HJ, Suh CH, Huh S, Nahm DH, Park HS. Clinical significance of serum ECP and sero-prevalence of human toxocariasis in patients with eosinophilia. J Asthma Allergy Clin Immunol 2003; 23: 26-32
  8. Kwon NH, Oh MJ, Lee SP, Lee BJ, Choi DC. The Clinical impact of toxocariasis in patients with unknown eosinophilia. J Asthma Allergy Clin Immunol 2005; 25: 299-304
  9. Maizels RM, Kennedy MW, Meghji M, Robertson BD, Smith HV. Shared carbohydrate epitopes on distinct surface and secreted antigens of the parasitic nematode Toxocara canis. J Immunol 1987; 139: 207-214
  10. Magnaval JF, Fabre R, Maurieres P, Charlet JP, de Larrard B. Application of the western blotting procedure for the immunodiagnosis of human toxocariasis. Parasitol Res 1991; 77: 697-702 https://doi.org/10.1007/BF00928685
  11. Nunes CM, Tundisi RN, Garcia JF, Heinemann MB, Ogassawara S, Richtzenhain LJ. Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique. Rev Inst Med Trop Sao Paulo 1997; 39: 253-256 https://doi.org/10.1590/S0036-46651997000500002
  12. Iddawela RD, Rajapakse RP, Perera NA, Agatsuma T. Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans. Korean J Parasitol 2007; Kim et al.: Seroprevalence of toxocariasis among healthy people 31 45: 19-26 https://doi.org/10.3347/kjp.2007.45.1.19
  13. Magnaval JF, Berry A, Fabre R, Morassin B. Eosinophil cationic protein as a possible marker of active human Toxocara infection. Allergy 2001; 56: 1096-1099 https://doi.org/10.1034/j.1398-9995.2001.00284.x
  14. Magnaval JF, Glickman LT, Dorchies P, Morassin B. Highlights of human toxocariasis. Korean J Parasitol 2001; 39: 1-11 https://doi.org/10.3347/kjp.2001.39.1.1
  15. Lim JH, Lee KS. Eosinophilic infiltration in Korea: idiopathic? Korean J Radiol 2006; 7: 4-6 https://doi.org/10.3348/kjr.2006.7.1.4