무청 추출물의 생리활성

Physiological Activity of Extracts from Radish (Raphanus sativus L.) Leaves

건조 무청을 물, 50% 에탄올 및 50% 메탄올로 추출한 물질의 생리활성 효과를 조사하였다. 건조 무청의 추출물 수율은 브랜칭하지 않은 건조 무청인 관동 무청(DRLK)은 $26.56{\sim}32.56%$이고, 미농단백 무청(DRLM)은 $34.34{\sim}36.90%$였다. 반면에 브랜칭한 무청군 수율은 $18.70{\sim}23.56%$로 브랜칭하지 않은 시료보다 수율이 낮았다. 총 폴리페놀 함량의 경우 브랜칭한 무청 시료는 $49.06{\sim}59.37mg/g$ 범위였고, 브랜칭을 하지 않은 시료는 $53.41{\sim}62.08mg/g$이었다. 시료에 따라서는 관동 무청시료(DRLK, BDRLK)보다 미농단백무 시료(DRLM, BDRLM)가 약간 더 높은 폴리페놀 함량을 나타내었다. 전자공여능은 브랜칭하지 않은 시료는 80% 이상의 활성을 보였고, 아질산염 소거작용은 $36.63{\sim}51.17%$ 범위의 활성을 보였으며, SOD 유사활성은 $38.53{\sim}45.38%$의범위의 활성을 보인 반면 브랜칭한 시료는 각각의 생리활성 측정에서 브랜칭하지 않은 시료보다 낮은 활성을 보였다. 전반적으로 관동 무청군이 미농단백 무청군보다 전자공여능, 아질산 소거능 및 SOD 유사활성에 있어서 약간 높은 활성을 보였고, 브랜칭하지 않은 무청이 높은 활성을 나타내었다.

This study was conducted to investigate yield of extract, total phenolic compounds content, electron donating activity, nitrite scavenging activity, superoxide dismutase like activity of the radish leaves (Raphanus sativus L.) extracts. Radish leaves extracts were prepared using water extraction, 50% ethanol extraction and 50% methanol extraction. The yield of Kwandong radish leaves extracts without blanching treatment (DRLK) was $26.56{\sim}32.56%$ and the extract yield of Minongdanbaek radish leaves without blanching treatment (DRLM) was $34.34{\sim}36.90%$. On the other hand, the yield of samples with blanching treatment was a lower range value of $18.70{\sim}23.56%$ than samples without blanching treatment. In the total contents of phenolic compound, samples with and without blanching treatment were $49.1{\sim}59.4mg/g\;and\;53.41{\sim}62.08mg/g$, respectively. Minongdanbaek radish leaves extracts (DRLM, BDRLM) showed higher contents value than Kwandong radish leaves extracts (DRLK, BDRLK) in the total phenolic compounds. Samples without blanching treatment showed electron donating activity above 80%, nitrite scavenging activity of $36.63{\sim}51.17%$ and superoxide dismutase like activity of $38.53{\sim}45.38%$. Generally, Kwandong samples showed a little higher activity more than Minongdanbaek samples in the electron donating activity, nitrite scavenging activity and superoxide dismutase like activity. Also, radish leaves extracts without blanching treatment showed high physiological activities.