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Cytotoxic and antioxidant properties of four plants belonging to the genus Solanum

  • Published : 2008.03.31

Abstract

The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with $IC_{50}$ values 31.25 $\pm$ 0.35, 40.33 $\pm$ 0.57 and 54.33 $\pm$ 0.57 ${\mu}g$/ml, respectively. Solanum barbisetum also showed potent activity in DPPH [1,1-diphenyl-2-picryl hydrazyl] method with an $IC_{50}$ value of 55.33 $\pm$ 1.66 ${\mu}g/ml. In the cytotoxicity studies, the methanol extract of Solanum barbisetum exhibited moderate activity against Vero, HEp-2, HeLa and A-549 cell lines with $IC_{50}$ values in the range of 83.30 - 127.30 ${\mu}g$/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with $IC_{50}$ value of 80.13 ${\mu}g$/ml. Solanum barbisetum possessing both the activities requires further investigation.

Keywords

References

  1. Andre CM, Ghislain M, Bertin P, Oufir M, Herrera MD, Hoffmann L, Hausman JF, Larondelle Y, Evers D. (2007) Andean potato cultivars (Solanum tuberosum L.) as a source of antioxidant and mineral micronutrients. J. Agric. Food Chem. 55, 366-378 https://doi.org/10.1021/jf062740i
  2. Anonymous. (1989) The Wealth of India, Vol. IX, Publication and Information Directorate, CSIR, New Delhi
  3. Badami S, Mahesh KG, Suresh B. (2003) Antioxidant activity of the ethanolic extract of Striga orobanchioides. J. Ethnopharmacol. 85, 227-230 https://doi.org/10.1016/S0378-8741(03)00021-7
  4. Barry H, John MCG, Okeizie IA. (1987) The deoxyribose method: A simple test tube assay for determination of rate constant for reactions of hydroxyl radicals. Anal. Biochem. 165, 215-219 https://doi.org/10.1016/0003-2697(87)90222-3
  5. Cragg GM, Newman DJ. (2005) Plants as a source of anticancer agents. J. Ethnopharmacol. 100, 72-79 https://doi.org/10.1016/j.jep.2005.05.011
  6. Elizabeth K, Rao MNA. (1990) Oxygen radical scavenging activity of curcumin. Int. J. Pharm. 58, 237-240 https://doi.org/10.1016/0378-5173(90)90201-E
  7. Francis D, Rita L. (1986) Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271-277 https://doi.org/10.1016/0022-1759(86)90368-6
  8. Garrat DC. (1964) The Quantitative Analysis of Drugs. $3^{rd}$ Ed. Chapman and Hall Ltd. Japan. pp. 456-458
  9. Guddadarangavvanahally KJ, Lingamallu JR, Kunnumpurath KS. (2006) Antioxidant activities of flavidin in different in vitro model systems. Bioorg. Med. Chem. 12, 5141-5146
  10. Halliwell B and Gutteridge J. (1999) Free Radicals in Biology and Medicine. 3rd edition, Oxford University Press, Oxford, U.K
  11. Hwang BY, Kim HS, Lee JH, Hong YS, Ro JS, Lee KS, Lee JJ. (2001) Antioxidant activity of benzoylated flavon-3-ol glycosides from Celastrus orbiculatus. J. Nat. Prod. 64, 82-84 https://doi.org/10.1021/np000251l
  12. Ikeda T, Tsumagari H, Honbu T, Nohara T. (2003) Cytotoxic activity of steroidal glycoalkaloids from Solanum plants. Biol. Pharm. Bull. 26, 1198-1201 https://doi.org/10.1248/bpb.26.1198
  13. Marcocci L, Packer L, Sckaki A, Albert GM. (1994) Antioxidant action of Ginkgo biloba extract Egb 766, Meth. Enzymol. 34, 462-475 https://doi.org/10.1016/0076-6879(94)34117-6
  14. Mohamed AF, Matina W, Gudrun S, Ulrike L. (2007) Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from Yemen. J. Ethnopharmacol. 111, 657-666 https://doi.org/10.1016/j.jep.2007.01.018
  15. Mohanan PV, Devi KS. (1996) Cytotoxic potential of the preparations from Solanum trilobatum and the effect of sobatum on tumour reduction in mice. Cancer Lett. 110, 71-76 https://doi.org/10.1016/S0304-3835(96)04463-1
  16. Pandey G. (2002) Anticancer herbal drugs of India with special reference to Ayurveda. New Delhi: Sri Satguru Publications, 18
  17. Re R, Pellegrini M, Proteggente A, Pannala A, Yang M, Rice EC. (1999) Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Radic. Biol. Med. 26, 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  18. Natesan S, Badami S, Dongre SH, Godavarthi A. (2007) Antitumor Activity and Antioxidant status of the methanol extract of Careya arborea bark against Dalton's Lymphoma Ascites-induced ascitic and solid tumor in mice. J. Pharmacol. Sci. 103, 12-23 https://doi.org/10.1254/jphs.FP0060907
  19. Skehan P, Storeng R, Scudiero D, Monks A, Mcmohan J, Vistica D. (1990) New colorimetric cytotoxic assay for anti-cancer drug screening. J. Natl. Cancer Inst. 82, 1107-1112 https://doi.org/10.1093/jnci/82.13.1107
  20. Shahjahan M, Sabitha KE, Mallika J, Shyamala DCS. (2004) Effect of Solanum trilobatum against carbon tetrachloride induced hepatic damage in albino rats. Indian J. Med. Res. 120, 194-198
  21. Shahjahan M, Vani G, Shyamaladevi CS. (2005) Effect of Solanum trilobatum on the antioxidant status during diethyl nitrosamine induced and phenobarbital promoted hepatocarcinogenesis in rat. Chem. Biol. Interact. 156, 113-123 https://doi.org/10.1016/j.cbi.2005.08.003
  22. Srivastava DSL, Mital RD. (2005) Free radical injury and antioxidant status in patients with benign prostate hyperplasia and prostate cancer. Indian J.\ Clin. Biochem. 20, 162-165 https://doi.org/10.1007/BF02867419
  23. Tripathi YB, Chaurasia S, Tripathi E, Upadhyay A, Dubey GP. (1996) Bacopa monniera as an antioxidant, mechanism of action. Indian J. Exp. Biol. 34, 523-526
  24. Vijayan P, Vinod Kumar S, Dhanraj SA, Badami S, Suresh B. (2002) In vitro cytotoxic and antitumor properties of the total alkaloid of unripe fruits of Solanum pseudocapsicum. Pharmaceutical Biol. 40, 456-460 https://doi.org/10.1076/phbi.40.6.456.8444
  25. Wei X, Li CG, Nong S, Zhu XY, Huang XM. (2006) The influence of Solanum lyratum Thunb extract on apoptosis and the expression of fas/fasL genes in Hela cells. Zhong Yao Cai 6, 1203-1206