Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Antimicrobial activity and cytotoxicity of Eclipta prostrata

  • Rahman, Mohammad S. (Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka) ;
  • Rashid, Mohammad A. (Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka)
  • Published : 2008.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The plant Eclipta prostrata, a member of the Compositae family, has folkloric reputation of being used as a medicinal agent in Bangladesh. In the present investigation, attempt was taken to explore the antimicrobial potency and cytotoxicity of its extractives and purified compounds. The methanolic extract of the whole plant, its n-hexane, carbon tetrachloride, chloroform, aqueous soluble fractions and two purified compounds, eclalbasaponin I (1) and II (2), obtained from Eclipta prostrata were subjected to screening for inhibition of microbial growth by the disc diffusion method at 300 and 100 ${\mu}g$/disc for extracts and pure compounds, respectively. In this case, the carbon tetrachloride and chloroform soluble fractions of the methanolic extract appeared very potent in terms of both zone of inhibition and spectrum of activity. However, all the extractives were also subjected to brine shrimp lethality bioassay for preliminary cytotoxicity evaluation. Here, the carbon tetrachloride soluble fraction of methanolic extract revealed the strongest cytotoxicity having $LC_{50}$ of 1.318 ${\mu}g$/ml.

Keywords

References

  1. Abdel-Kader MS, Bahler BD, Malone S, Werkhoven MC, van Troon F, Wisse DJ, Bursuker II, Neddermann KM, Mamber SW, Kingston DG. (1998) DNADamaging Steroidal Alkaloids from Eclipta alba from the Suriname rainforest. J. Nat. Prod. 61, 1202-1208 https://doi.org/10.1021/np970561c
  2. Austin DJ, Kristinsson KG, Anderson RM. (1999) The relationship between the volume of antimicrobial consumption in human communities and the frequency of resistance. Proc. Natl. Acad. Sci. U.S.A. 96, 1152-1156 https://doi.org/10.1073/pnas.96.3.1152
  3. Bauer AW, Kirby WMM, Sherries JC, Truck M. (1966) Antibiotic susceptibility testing by standard single disc diffusion method. Am. J. Clin. Pathol. 45, 426-493
  4. Chevallier A. (1996) The Encyclopedia of Medicinal Plants. Dorling Kindersley. London. ISBN 9-780751-303148
  5. Chopra RN, Nayar SL, Chopra IC. (1992) Glossary of Indian Medicinal Plants, $3^{rd}$ edition, CSIR, New Delhi, India
  6. Cowan MM. (1999) Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12, 564-582
  7. Cox PA. (1994) The ethnobotanical approaches to drug discovery: strengths and limitations. In: Ethnobotany and the Search for new Drugs, Ciba Foundation, John Wiley and Sons Ltd., West Sussex, England https://doi.org/10.1002/9780470514634.ch3
  8. Dictionary of Natural Products, Chapman and Hall, 2002
  9. Farnsworth NR. (1990) The role of enthnopharmacology in drug development: In: Bioactive compounds from plants, Eds. Chadwick, D.J. and Marsh, J. Wiley –interscience publications. U.K
  10. Ghani A. (2003) Medicinal Plants of Bangladesh: Chemical Constituents and Uses, $2^{nd}$ edition, Asiatic Society of Bangladesh, p. 216-217 and references cited therein
  11. Hamil FA, Apio S, Mubiru NK, Bukenya-Ziraba R, Mosango M, Maganyi OW, Soejarto DD. (2003) Traditional herbal drugs of Southern Uganda, II: literature analysis and antimicrobial assays. J. Ethnopharmacol. 84, 57-78 https://doi.org/10.1016/S0378-8741(02)00289-1
  12. McLaughlin JL. (1991) Bench-top bioassays for the discovery of bioactive compounds in higher plants. Brenesia 34, 1-14
  13. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen JB, Nicholsand DE, McLaughlin JL. (1982) Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 45, 31-34 https://doi.org/10.1055/s-2007-971236
  14. Motsei ML, Lindsey KL, Van Staden J, Jaeger AK. (2003) Screening of traditionally used South African plants for antifungal activity against Candida albicans. J. Ethnopharmacol. 86, 235-241 https://doi.org/10.1016/S0378-8741(03)00082-5
  15. Persoone G. (1980) Proceeding of the international symposium on brine shrimp, Artemia salina, pp. 1-3, University Press
  16. Rahman MS, Chowdhury R, Hasan CM, Rashid MA. (2006) Oleanane Glycosides from Eclipta prostrata. Dhaka Univ. J. Pharm. Sci. 4, 107-111
  17. Rahman S, Hasnat CM, Rashid MA, Ilias, M. (2001) Pharmacological evaluation of Bangladeshi medicinal plants- a review. Pharm. Biol. 39, 1-6 https://doi.org/10.1076/phbi.39.1.1.5939
  18. Rashid MA, Hasan CM, Choudhury SAR, Begum B, Rahman S. (1997) Ethnopharmacological investigation of medicinal plants of Bangladesh. Bangladesh J. Physiol. Pharmacol. 12, 25-29
  19. Saraf AP, Joglekar SN, Nabar, SD, Mulay MS. (1991) Evaluation of protective effect of 'Hepatogard' a compound herbal formulation in carbon tetrachloride induced liver damage. Antiseptic 88, 511-516
  20. Satyavati GV, Rana MK, Sharma M. (1976) In: Medicinal plants of India. Cambridge Printing Works, Vol. I, Delhi, pp. 370-372
  21. Singh B, Saxena AK, Chandan BK, Agarwal SG, Bhatia MS, Anand KK. (1993) Hepatoprotective effect of ethanolic extract of Eclipta alba on experimental liver damage in rats and mice. Phytother. Res. 7, 154-158 https://doi.org/10.1002/ptr.2650070212
  22. Uniyal RC, Sandhu S, Chandok JK. (1998) Herbology. In: The Ayurvedic Encyclopedia. Sri Satguru Publications, India, p.77
  23. Upadhyay RK, Pandey MB, Jha RN, Pandey VB. (2001) Eclalbatin, a triterpene saponin from Eclipta alba. J. Asian Nat. Prod. Res. 3, 213-217 https://doi.org/10.1080/10286020108041393
  24. Vaidya AB. (1997) The status and scope of Indian medicinal plants acting on central nervous system. Indian J. Pharmacol. 29, 340-343
  25. Vanwagenen BC, Larsen R, Cardellina, JH II, Randazzo D, Lidert ZC, Swithenbank C. (1993) Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri. J. Org. Chem. 58, 335-337 https://doi.org/10.1021/jo00054a013
  26. Wagner H, Geyer B, Kiso Y, Hikino H, Rao GS. (1986) Coumestans as main active principles of the liver drugs Eclipta alba and Wedelia calendulaceae. Planta Med. 5, 370-373 https://doi.org/10.1055/s-2007-969188
  27. Yahara S, Ding N, Nohara T. (1994) Oleanane glycosides from Eclipta alba Chem. Pharm. Bull. 42, 1336-1338

Cited by

  1. Evaluation of Antibacterial and Antidiarrhoeal Activities of <i>Feronia limonia</i> Leaf Extract vol.04, pp.11, 2013, https://doi.org/10.4236/ajps.2013.411270
  2. Ex-vivo cytotoxic, antibacterial and DPPH free radical scavenging assay with ethanolic leaf extract of Glycosmis pentaphylla to justify its traditional use vol.2, pp.7, 2015, https://doi.org/10.7603/s40730-015-0017-x