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Study on diuretic activity and electrolytes excretion of methanol extract of Lippia nodiflora (Verbenaceae) in rats

  • D., Ashok Kumar (Department of Pharmaceutical Technology, Jadavpur University) ;
  • GP, Senthilkumar (Department of Pharmaceutical Technology, Jadavpur University) ;
  • V., Thamil selvan (Department of Pharmaceutical Technology, Jadavpur University) ;
  • UK, Mazumder (Department of Pharmaceutical Technology, Jadavpur University) ;
  • M., Gupta (Department of Pharmaceutical Technology, Jadavpur University) ;
  • SK, Ray (Central Drug Laboratory)
  • Published : 2008.03.31

Abstract

In the Indian traditional medicine, Lippia nodiflora (Verbenaceae) whole plant is claimed to possess powerful diuretic activity. However, the diuretic potential of this plant is not yet investigated. The aim of this study was to evaluate the diuretic potential of methanol extract of Lippia nodiflora (MELN) in rats. Control (0.9% saline solution, 25 ml/kg, b.w) or urea (1 g/kg b.w) or frusemide (5 mg/kg b.w) and different concentrations of MELN (200 and 400 mg/kg b.w) were intraperitoneally administered (n = 6 per each treatment group) to hydrated rats and their urine output was monitored over a period of 5 h and 24 h after drug administration. The diuretic responses with its electrolyte excretion potency of the extract were highly remarkable in comparison with control animals. The extract at doses of 200 and 400 mg/kg shows a significant increase in volume of urine with increase in $Na^{+}$, $Ca^{2+}$ and $Cl^{-}$ excretion accompanied by the excretion of $K^{+}$ in dose dependent manner. This study suggests that the active component(s) in MELN had similar diuretic effect to that of frusemide. These results validate the traditional use of Lippia nodiflora as a diuretic agent.

Keywords

References

  1. Al-Ali M, Wahbi S, Twaij H, Al-Badr A. (2003) Tribulus terrestris: Preliminary study of its diuretic and contractile effects and comparision with Zea mays. J. Ethnopharmacol. 85, 257-260 https://doi.org/10.1016/S0378-8741(03)00014-X
  2. Alekutty NA, Srinivasan KK, Gundu Rao P, Udupa AC, Keshavamurthy KR. (1993) Diuretic and antilithiatic activity of Dendrophthoe falcate. Fitoterapia 64, 325-331
  3. Bevevino LH, Vieira FSA, Cassola AC, Sanioto SML. (1994) Effect of crude extract of roots of Bredemeyera floribunda Willd. J. Ethnopharmacol. 43, 197-201 https://doi.org/10.1016/0378-8741(94)90043-4
  4. Caceres A, Fletes L, Aquilar L, Ramirez O, Figueroa L, Taracena AM, Samaya B. (1993) Plants used in Guatemala for the treatment of gastrointestinal disorders. Confirmation of activity against enterobacteria of 16 plants. J. Ethnopharmacol. 38, 31-38 https://doi.org/10.1016/0378-8741(93)90076-H
  5. Chodera A, Dabrowska K, Sloderbach A, Skrzypczak L, Budzianowski J. (1991) Effect of flavanoid fractions of Solidago virgaurea L. on diuresis and levels of electrolytes. Acta. Pol. Pharm. 48, 35-37
  6. Chopra RN, Nayar SL, Chopra IC. (1956) Glossary of Indian Medicinal Plants, pp. 155 C.S.I.R, New Delhi
  7. Chopra RN, Chopra IC, Handa KL, Kapur LD. (1958) Indigenous Drugs of India, Second Edition, pp. 598 U.N.Dhur and Sons Private Limited, Calcutta
  8. Das AK, Shahid IZ, Choudhuri MSK, Shilpi JA, Firoj Ahmed. (2005) Anti-inflammatory, antinociceptive and diuretic activities of Amoora Cucullata Roxb. Orient. Pharm. Exp. Med. 5, 37-42
  9. Gamble JS. (1957) Flora of the Presidency of Madras, Vol II, pp. 762 Botanical survey of India, Calcutta
  10. Ecobichon DJ. (1997) The basis of toxicity testing, pp. 43-86 CRC Press, New York
  11. Forestieri AM, Monforte MT, Ragusa S, Trovato A, Iauk L. (1996) Antiinflammatory, analgesic and antipyretic activity in rodents of plant extracts used in African medicine. Phytother. Res. 10, 100-106 https://doi.org/10.1002/(SICI)1099-1573(199603)10:2<100::AID-PTR724>3.0.CO;2-I
  12. De Stevens G. (1963) Diuretics: In: Chemistry and Pharmacology, $1^{st}$ed. p.2-7 and 52-58, Academic Press, New York
  13. Gujral ML, Saxena PN, Misra SS. (1955) An experimental study of the comparative activity of indigenous diuretics. J. Indian Med. Assoc. 25, 49-51
  14. Harvey SK. (1966) A preliminary experimental study of the diuretic activity of some indigenous drug. Indian J. Med. Res. 54, 774-778
  15. Horisberger JD, Giebisch G. (1987) Potassium sparing diuretics. Ren. Physiol. 10, 198-220
  16. Indian Pharmacopoeia. (1996) Publications and Information Directorate, pp. 689 CSIR, New Delhi
  17. Jackson EK. (1996) Drugs affecting renal and cardiovascular function. In: Goodman and Gilman's The Pharmacological Basis of Therapeutics edited by Hardman JG, Gilman AG, Limbird LE$9^{th}$ed. p. 685-713 McGraw-Hill, New York
  18. Jackson EK. (2001) Diuretics. In: Goodman and Gilman's The Pharmacological Basis of Therapeutics edited by Hardman JG, Limbird LE, Goodman GA p. 755-788 McGraw-Hill, New York
  19. Kirthikar KR and Basu BD. (1975) Indian Medicinal Plants, Vol III, 1915-1917 pp. 1915-1917 Bishen Mahendra Pal Singh, Dehradun
  20. Kokate CK. (1997) Practical Pharmacognosy, pp.110-111 Vallabh Prakashan, Delhi
  21. Lipschitz WL, Hadidian Z, Kerpesar A. (1943) Bioassay of diuretics. J. Pharmacol. Exp. Ther. 79, 97-110
  22. Leuschner J. (1995) Anti-inflammatory, spasmolytic and diuretic effects of a commercially available Solidago gigantea Herb Extract. Arzneim-Forsch. Drug Res. 45, 15-168
  23. Mamun MM, Billah MM, Ashek MA, Ahasan MM, Hossain MJ, Sultana T. (2003) Evaluation of diuretic activity of Ipomoea aquatica (Kalmisak) in mice model study. J. Med. Sci. 3, 395-400
  24. Milton JC, Sheldon M, Brainerd H. (1970) Handbook of Medical Treatment $12^{th}$ed pp.220-229 Lange Publications, California
  25. Mukherjee PK, Pal M, Saha K, Saha BP. (1996) Diuretic activity of extract of the rhizomes of Nelumbo nucifera Gaertn. (Fam. Nymphaeaceae). Phytother. Res. 10, 424-425 https://doi.org/10.1002/(SICI)1099-1573(199608)10:5<424::AID-PTR857>3.0.CO;2-3
  26. Mukherjee T. (1991) Antimalarial herbal drugs. A review. Fitoterapia 62, 197-204
  27. Murugesan T, Manikandan L, Suresh, KB, Pal M, Saha BP. (2000) Evaluation of diuretic potentials of J. suffruticosa Linn extracts in rats. Indian J. Pharm. Sci. 62, 150
  28. Nadkarni AK. (1954) Indian Materia Medica, pp. 746 Popular Prakashan, Bombay, India
  29. Nilveses N, Wammanachinda W, Wanverakul B, Pidech P. (1989) Diuretic effect of Pluchea indica. Thai journal of Pharmacology. 11, 1-7
  30. OECD. (1996) OECD Guidelines for the testing of chemicals Test No 423: Acute oral toxicity- Acute toxic classic method
  31. Pantoja CV, Chiang LCH, Norris BC, Concha JB. (1991) Diuretic, natriuretic and hypotensive effects produced by Allium sativum (Garlic) in anesthetized dogs. J. Ethnopharmacol. 31, 325-331 https://doi.org/10.1016/0378-8741(91)90018-9
  32. Ramachandra K. (1989) Useful Plants of India, pp. 246 Publications and Information Directorate, CSIR, New Delhi
  33. Rang HP, Dale MM, Ritter JM, Moore PK. (2003) Pharmacology, Fifth edition, pp. 352-366 Elsevier Science Limited, United Kingdom
  34. Rani A. (1988) Experimental evaluation of antilithiatic activity of Homonia repeoria lour in male albino rats [Dissertation] Mangalore University, Manipal
  35. Rizvi SH, Shoeb A, Kapil RS, Satya P. (1980) Two diuretic triterpenoids from Antiderma menasu. Phytochemistry 19, 2409-2410 https://doi.org/10.1016/S0031-9422(00)91037-9
  36. Sheth UK, Dadkar NK, Kamat UG. (1972) Selected Topics in Experimental Pharmacology. pp. 164-166 Kothari Book Depot, Bombay
  37. Singh RH, Udupa KN. (1972) Studies on the Indian indigenous drug, Punarnava (Boerhavvia diffusa Linn). J. Res. Indian Med. 7, 29-31
  38. Singh RG, Singh RP, Usha KP. (1991) Experimental evaluation of diuretic action of herbal drug (Tribulus terrestris Linn.) on albino rats. J. Res. Educ. Indian Med. 3, 19-21
  39. Somova LI, Shode FO, Ramanan P, Nadar A. (2003) Antihypertensive, antiatherosclerotic and antioxidant activity of triterpenoids isolated from Olea europaea, subspecies Africana leaves. J. Ethnopharmacol. 84, 299-305 https://doi.org/10.1016/S0378-8741(02)00332-X
  40. Sood AR, Bajpai A and Digits M. (1985) Pharmacological and biological studies on saponins. Indian J. Pharmacol. 17, 178-179
  41. Srivastava R, Shukla YN, Kumar S. (1988) Chemistry, Pharmacology and botany of Boerhavvia diffusa. J. Medicinal Aromatic Plant Sci. 20, 762-766
  42. Stanton BA, Giebisch GH. (1992) Renal potassium transport. In: Handbook of Physiology. Section 8: Renal Physiology edited by Windhager, E.E p. 813-874 Oxford University Press, New York
  43. The Useful Plants of India. (1986) Third Edition, pp. 450 Publication and Information Directorate, Council of Scientific and Industrial Research, New Delhi
  44. Van Armar GG. (1954) The diuretic activity of isocytosines. J. Pharmacol. Exp. Ther. 111, 285-292
  45. Wealth of India-Raw materials. (1962) Vol VI, pp. 142-143 Publication and Information Directorate, Council of Scientific and Industrial Research, New Delhi
  46. Wilson RW, Wareing M, Green R. (1997) The role of active transport in potassium reabsortption in the proximal convoluted tubule of the anesthetized rat. J. Physiol. 500, 155-164
  47. Zafar R. (1994) Medicinal Plants of India. 1st Ed, pp. 361-362 CBS Publications, New Delhi
  48. Zamora-Martinez MC, Nieto de Pascual C. (1992) Medicinal plants used in some rural populations of Oaxaca, Publa and Veracruz, Mexico. J. Ethnopharmacol. 35, 229-257 https://doi.org/10.1016/0378-8741(92)90021-I