Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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In Vitro Activity of Methyl Gallate Isolated from Galla Rhois Alone and in Combination with Ciprofloxacin Against Clinical Isolates of Salmonella

  • Choi, Jang-Gi (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kang, Ok-Hwa (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Lee, Young-Seob (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Oh, You-Chang (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Chae, Hee-Sung (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Jang, Hye-Jin (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Jong-Hak (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Sohn, Dong-Hwan (Department of Pharmacy, College of Pharmacy, Wonkwang University) ;
  • Shin, Dong-Won (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Park, Hyun (Department of Parasitology, College of Medicine, Wonkwang University) ;
  • Kwon, Dong-Yeul (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University)
  • Published : 2008.11.30
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Abstract

Salmonella remains a primary cause of food poisoning worldwide, and massive outbreaks have been witnessed in recent years. Therefore, this study investigated the antimicrobial activity of methyl gallate (MG), which exhibited good antibacterial activity ($MIC=3.9-125{\mu}g/ml$) against all the bacterial strains tested. In a checkerboard dilution test, MG markedly lowered the MICs of ciprofloxacin (CPFX) against Salmonella. The combined activity of CPFX and MG against Salmonella resulted in fractional inhibitory concentrations (FICs) ranging from 0.0037 to 0.015 and from 0.24 to $7.8{\mu}g/ml$, respectively. Meanwhile, the FIC index ranged from 0.31-0.37, indicating a marked synergistic relationship between CPFX and MG against Salmonella. Time-kill assays also showed a decrease in the CFU/ml between the combination and the more active compound. Therefore, this study demonstrated that MG and CPFX can act synergistically in inhibiting Salmonella in vitro.

Keywords

References

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