Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Regulation of $CO_2$ Fixation Gene Expression in Acidithiobacillus ferrooxidans ATCC 23270 by Lix984n Shock

  • Wang, Wei (School of Minerals Processing and Bioengineering, Central South University) ;
  • Xiao, Shuiming (School of Minerals Processing and Bioengineering, Central South University) ;
  • Chao, Jing (School of Minerals Processing and Bioengineering, Central South University) ;
  • Chen, Qijiong (School of Minerals Processing and Bioengineering, Central South University) ;
  • Qiu, Guanzhou (School of Minerals Processing and Bioengineering, Central South University) ;
  • Liu, Xueduan (School of Minerals Processing and Bioengineering, Central South University)
  • Published : 2008.11.30
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Abstract

Acidithiobaeillus ferrooxidans ATCC 23270 is an important model organism for bioleaching and bioremediation studies owing to its diverse metabolic capabilities, whereas lix984n is a widely used extractant. Little is known about the response of cbb genes in A. ferrooxidans to lix984n shock. Thus, to elucidate the response of the $CO_2$ fixation genes in A. ferrooxidans ATCC 23270 to the addition of lix984n, the gene expression of cbb genes was examined using a real-time PCR. Although a natural increase or decrease in the expression of most cbb genes was observed after 5 min of shock with 3% (v/v) lix984n, sdhC and cbbR exhibited quick responses to the shock. Ten min of shock had a greater effect on the cbb gene expression, yet 15 min of shock had a significant effect on the Calvin cycle in A. ferrooxidans ATCC 23270, as the expression of all the cbb genes reached a very high level. Therefore, after a short lix984n shock, a solution of A. ferrooxidans can be re-used for bioleaching.

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References

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