Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation and Improvement of Bioassay for Residual Antibiotics in Foods

식품 내의 잔류 항생제에 대한 미생물학적 간이검사법의 평가 및 개선

  • Park, Min-Hee (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Tae-Woon (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Jo, Nam-Uk (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Jeong, Ji-Yoon (Pesticide & Veterinary Drug Residues Division, Korea Food and Drug Administration) ;
  • Lee, Soon-Ho (Pesticide & Veterinary Drug Residues Division, Korea Food and Drug Administration) ;
  • Lee, Jong-Ok (Pesticide & Veterinary Drug Residues Division, Korea Food and Drug Administration) ;
  • Kim, Hae-Yeong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
  • 박민희 (경희대학교 생명자원과학연구원 및 생명공학원) ;
  • 김태운 (경희대학교 생명자원과학연구원 및 생명공학원) ;
  • 조남욱 (경희대학교 생명자원과학연구원 및 생명공학원) ;
  • 정지윤 (식품의약품안전청 식품잔류약품과) ;
  • 이순호 (식품의약품안전청 식품잔류약품과) ;
  • 이종옥 (식품의약품안전청 식품잔류약품과) ;
  • 김해영 (경희대학교 생명자원과학연구원 및 생명공학원)
  • Published : 2008.12.28
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Abstract

For the screening of residual antibiotics in foods, bioassays and microbiological inhibitor tests are commonly applied. These methods are tested by the various susceptibility of bacteria against different kinds of antibiotics. However, the sensitivity of bioassay is generally insufficient to detect some residual antibiotics at level of interest. This study was performed to investigate the detection limit of variable antibiotics of the bioassay and to improve the sensitivity to some antibiotics. The sensitivity of bioassay using Bacillus megaterium ATCC 9885, B. subtilis ATCC 6633, B. cereus ATCC 11778 and Geobacillus stearothermophilus ATCC 10149 was low in the detection of macrolides, quinolones, chloramphenicol, and monensin. On the contrary, Micrococcus luteus ATCC 9341 showed high sensitivity to macrolides and Escherichia coli ATCC 11303 was highly sensitive to quinolones and aminoglycosides. Consequently, both strains would be useful to improve sensitivity of bioassay with a wide detection range.

식품 내의 잔류 항생물질의 검사는 주로 미생물의 생육 억제 여부를 통한 생물학적인 분석을 이용한다. 이러한 방법은 여러 가지 계열의 항생제들에 대한 미생물의 민감성을 토대로 이용되고 있다. 그러나 현재 사용하고 있는 미생물들로는 동물용의약품으로 허가되어 식용동물에 사용되고 있는 점점 다양해지는 항생제 및 합성항균제를 식품에서 모두 검출할 수는 없다는 한계가 있다. 그러므로 본 연구는 검출 가능한 항생제 및 합성항균제 계열의 범위를 확대하고, 각 약품별 감도를 증진시키기 위한 새로운 방법들을 조사하였다. B. megaterium ATCC 9885, B. subtilis ATCC 6633, B. cereus ATCC l1778 and Geobacillus stearothermophilus (B. stearothermophilus) ATCC 10149를 사용한 검사의 민감성은 macrolides, quinolones와 monensin, chloramphenicol에 대한 검출 감도가 낮았다. 반면에 M. luteus(K. rhizophila) ATCC 9341는 macrolides에 대한 높은 검출 감도를 나타냈고, E. coli ATCC l1303는 quinolones와 aminoglycosides에 대한 높은 민감성을 나타냈다. 결론적으로, 두 균주의 추가로 검출가능한 항생제 및 합성항균제의 범위를 확대하였으며, 검출감도를 높일 수 있게 되었다.

Keywords

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