한국발생생물학회지:발생과생식 (Development and Reproduction)
- 제12권2호
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- Pages.141-149
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- 2008
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- 2465-9525(pISSN)
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- 2465-9541(eISSN)
동결에 앞서 시행된 지지세포와의 추가 공배양이 인간 배아줄기세포의 유리화 동결보존 후 생존율과 미분화 유지에 미치는 영향
Improving the Survival and Maintenance of the Undifferentiated State of Cryopreserved Human Embryonic Stem Cells by Extended Incubation with Feeder Cells Overnight before Vitrification
- 차수경 (포천중문의과대학교 차병원 여성의학연구소) ;
- 최경희 (포천중문의과대학교 차병원 여성의학연구소) ;
- 신주미 (포천중문의과대학교 차병원 여성의학연구소) ;
- 박규형 (포천중문의과대학교 줄기세포연구소) ;
- 윤태기 (포천중문의과대학교 차병원 여성의학연구소) ;
- 정형민 (포천중문의과대학교 줄기세포연구소) ;
- 이동률 (포천중문의과대학교 차병원 여성의학연구소)
- Cha, Soo-Kyung (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University College of Medicine) ;
- Choi, Kyoung-Hee (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University College of Medicine) ;
- Shin, Ju-Mi (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University College of Medicine) ;
- Park, Kyu-Hyung (CHA Stem Cell Institute, Pochon CHA University College of Medicine) ;
- Yoon, Tae-Ki (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University College of MedicineCHA Stem Cell Institute, Pochon CHA University College of Medicine) ;
- Chung, Hyung-Min (CHA Stem Cell Institute, Pochon CHA University College of Medicine) ;
- Lee, Dong-Ryul (Fertility Center of CHA General Hospital, CHA Research Institute, Pochon CHA University College of Medicine)
- 발행 : 2008.09.30
초록
This study was conducted to develop an efficient cryopreservation method of human embryonic stem (ES) cells using vitrification. In an initial experiment, sub-clumps of human ES cells (CHA-hES3 and CHA-hES4) were vitrified using grids after incubation with STO feeder cells for 1 or 16 h (Groups 1-1 and 1-2, respectively). After storage for
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