Journal of environmental and Sanitary engineering (환경위생공학)

Korean Society for Environmental Sanitary Engineers (대한환경위생공학회)
권호 표지

Antimicrobial Agent and Chlorine Susceptibility of E. coli Group Isolated from Natural Drinking Water in Northern Gyeongbuk Area

경북북부지역 먹는물에서 분리된 대장균군의 항균제 및 염소소독제 감수성

  • Sohn, Chang-Kyu (Gyeongsangbuk-Do institute of health and environment, northern branch) ;
  • Lee, Chun-Woo (Gyeongsangbuk-Do institute of health and environment, northern branch) ;
  • Kim, Tae-Bun (Gyeongsangbuk-Do institute of health and environment, northern branch) ;
  • Park, Soon-Geel (Gyeongsangbuk-Do institute of health and environment, northern branch) ;
  • Jeon, Chan-Jun (Gyeongsangbuk-Do institute of health and environment, northern branch) ;
  • Lee, Chang-Il (Gyeongsangbuk-Do institute of health and environment, northern branch) ;
  • Park, Ja-Young (Gyeongsangbuk-Do institute of health and environment, northern branch) ;
  • Song, Sung-Bok (Gyeongsangbuk-Do institute of health and environment, northern branch) ;
  • Huh, Wan (Gyeongsangbuk-Do institute of health and environment, northern branch)
  • 손창규 (경상북도보건환경연구원북부지원) ;
  • 이춘우 (경상북도보건환경연구원북부지원) ;
  • 김태분 (경상북도보건환경연구원북부지원) ;
  • 박순길 (경상북도보건환경연구원북부지원) ;
  • 전찬준 (경상북도보건환경연구원북부지원) ;
  • 이창일 (경상북도보건환경연구원북부지원) ;
  • 박자영 (경상북도보건환경연구원북부지원) ;
  • 송성복 (경상북도보건환경연구원북부지원) ;
  • 허완 (경상북도보건환경연구원북부지원)
  • Published : 2008.06.30
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Abstract

We examined 165 unchlorinated natural drinking water samples for the presence of E. coli group resistant to antimicrobial agent and chlorine in nothern Gyeongbuk area in 2007. Among 165 water samples, 21 samples(12.7%) were positive to total coliforms and Six genus, 16 strains of E. coli groups isolated from 16 samples showed resistance against more than one antimicrobial agent such as Ampicillin, Tetracycline and Chloroamphenicol. Among 16 strains, 14 strains resistant to Ampicillin, 9 strains resistant to Tetracycline and one strain resistant to Chloroampenicol. but all 16 strains did not contain any integron gene cassettes, which contribute to the spread of antimicrobial resistance alleles by lateral gene transfer of gene cassettes in a variety of enteric bacteria. The minimal inhibitory concentration(MIC) of 14 strains which showed resistant to Ampicillin was between $12{\mu}g/m{\ell}$ and $32{\mu}g/m{\ell}$, Nine strains resistant to Tetracycline showed between $32{\mu}g/m{\ell}$ and $128{\mu}g/m{\ell}$ and one strain resistant to Chloroampenicol showed $128{\mu}g/m{\ell}$. The chlorine sensitivity of 16 strains isolated from unchlorinated natural water sample did not show any difference among strains by the concentration of initial free chlorine and elapsed time after chlorine treatment. All 16 strains were killed after 1hr. exposure at $0.2mg/m{\ell}$ of free chlorine per liter or 30minutes exposure at $0.4mg/m{\ell}$ of free chlorine per liter.

Keywords

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