Radiation Oncology Journal

The Korean Society for Radiation Oncology (대한방사선종양학회)
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Lack of EGCG Effects on Radiation-Induced Apoptosis of Mice Splenocytes

마우스 비장세포에서 방사선유도성 아포토시스에 대한 EGCG 효과의 부족

  • Jang, Seong-Soon (Department of Radiation Oncology, College of Medicine, The Catholic University of Korea) ;
  • Lee, Heui-Kwan (Department of Radiation Oncology, College of Medicine, The Catholic University of Korea)
  • 장성순 (가톨릭대학교 의과대학 방사선종양학교실) ;
  • 이희관 (가톨릭대학교 의과대학 방사선종양학교실)
  • Published : 2008.09.30
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Abstract

Purpose: The modification of radiation-induced apoptosis by EGCG, known as antioxidants or oxidants, was studied in mice spleens irradiated with a lethal dose. Materials and Methods: Male C57BL/6 mice were divided into control, irradiation-only, and EGCG (100 mg/kg i.p. 1 h before irradiation) pretreatment groups. The mice were irradiated with a single whole-body dose of 7 Gy. The apoptosis in the spleens after irradiation of the lethal dose were analyzed by TUNEL assay. In addition, the expression levels of the Bax and Bcl-2 proteins were quantified using a Western blotting method. Results: The induction of apoptosis was detected in the splenic white pulp. The highest level of apoptosis was detected at 8 hours after irradiation. No significant difference was identified by the apoptotic index (53.9% vs. 52.1%, p=0.328) and relative Bax protein expression (0.86 vs. 0.81, p=0.335), between the irradiation-only and EGCG pretreatment group, respectively. However, a lower Bax/Bcl-2 ratio (1.64 vs. 0.97, p=0.037) and higher relative expression level of Bcl-2 protein (0.57 vs. 0.82, p=0.037) was measured in the EGCG pretreatment group. Conclusion: The EGCG pretreatment neither decreased the radiation-induced apoptosis in mice splenocytes, nor induced additional apoptosis.

목 적: 치사선량의 방사선에 의해 유도되는 아포토시스에 대해 항산화제 또는 산화제로 알려진 EGCG가 어떤 효과를 나타내는지를 마우스 비장에서 연구하였다. 대상 및 방법: C57BL/6 마우스를 실험군에 따라 정상대조군, 방사선조사 단독군, EGCG 전처치군(방사선조사 1시간 전에 100 mg/kg 복강주사)으로 분류하고 7 Gy의 단일선량으로 전신조사 하였다. 각 실험군별 비장 내의 아포토시스는 TUNEL assay를 통해 분석하였고, Bax와 Bcl-2 유전자 발현은 Western blotting 방법으로 측정하였다. 결 과: 비장 내 아포토시스는 주로 white pulp에만 국한되어 발견되었으며, 방사선조사 후 8시간째에 최대의 아포토시스지수를 나타냈다. 8시간째에 방사선조사 단독군과 EGCG 전처치군의 아포토시스지수는 53.9%와 52.1%로 유의한 차이를 보이지 않았다(p=0.328). 또한 두군간에 Bax/Bcl-2 ratio (1.64 vs 0.97, p=0.037)와 대조군에 상대적인 Bcl-2 단백질 발현(0.57 vs 0.82, p=0.037)에서는 유의한 차이를 보였으나, Bax 발현(0.86 vs 0.81, p=0.335)에서는 차이를 보이지 않았다. 결 론: 마우스 비장세포에서 EGCG가 방사선에 의해 유도되는 아포토시스를 유의하게 감소시키지 못하였고, 추가적인 아포토시스의 증가를 유발하지도 않았다.

Keywords

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