Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Phylogenetic Analysis of Ji-Mo (Anemarrhena asphodeloides) on the Basis of Chloroplast DNA Sequences

엽록체 DNA 염기서열을 이용한 한약재 지모의 기원 확인 및 유연관계 분석

  • Kim, Myung-Kyum (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank) ;
  • Jigden, Baigalmaa (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank) ;
  • Sun, Hua (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank) ;
  • Noh, Jong-Hun (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank) ;
  • Kim, Se-Young (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank) ;
  • Yang, Deok-Chun (Korea Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank)
  • 김명겸 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 베갈마 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 손화 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 노종훈 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 김세영 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행) ;
  • 양덕춘 (경희대학교 고려인삼명품화사업단 및 인삼유전자원소재은행)
  • Published : 2008.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

Anemarrhena asphodeloides (Korean name "Ji-Mo") has been used for oriental medicinal purposes in Korea, China and Japan. In this study, 29 A. asphodeloides samples were collected including 3 certified A. asphodeloides plants and many commercially marketed A. asphodeloides products. Chloroplast trnL-F regions of the "Ji-Mo" samples were sequenced and used to identify whether the samples were genuine A. asphodeloides or not. As the result, the trnL-F sequences of all the "Ji-Mo" samples were shown to be identical and it was proven that commercially available medicinal products "Ji-Mo" are genuine A. asphodeloides. Phylogenetic tree of. A. asphodeloides using the trnL-F sequences was constructed and compared with phylogenetic tree using rubisco large subunit (rbcL) gene sequences. In these tree, A. asphodeloides was affiliated in the family Agavaceae in the order Asparagales. It is proven that trnL-F phylogenetic tree is useful to study taxonomic position of A. asphodeloides.

지모 (Anemarrhena asphodeloides)는 탁월한 해열작용과 진정작용을 갖는 한약재로 한국, 중국, 일본에서 널리 이용되어 왔다. 본 연구에서는 먼저 국내 연구소에서 형태학적 분류 결과 지모로 확인된 3종의 식물체를 수집하여 엽록체 DNA의 trnL-F 염기서열을 분석하였다. 분석 결과, 국내 연구기관에서 보관중인 지모 식물체들이 모두 동일한 trnL-F의 염기서열을 보여서, 형태학적 분류와 계통유전학적 분류가 동일함을 확인하였다. 최초로 얻어진 지모 trnL-F 염기서열은 NCBI database에 등록하였다. 다음으로 국내 한약재 시장과 중국 한약재 시장에서 유통 중인 지모 한약재를 다량 구입하여 trnL-F의 염기서열을 분석하였다. 그 결과, 유통 중인 지모 한약재들이 모두 기원식물과 동일한 TrnL-F의 염기서열을 보여서 지모 약재의 경우 진품이 유통되고 있음을 알 수 있었다. TrnL-F의 염기서열로 계통수를 작성한 결과 지모는 아스파라거스목 (Asparagales), 용설란과 (agavaceae)에 속한 것으로 보여 졌다. 엽록체 rbcL 유전자 염기서열로 얻은 계통수와 비교한 결과 trnL-F 계통수와 rbcL 계통수가 비슷한 결과를 보여주어서 분자유전학적 분류에 두 유전자가 상호보완적으로 이용될 수 있음을 확인하였다.

Keywords

References

  1. An SM, Ryuk JA, Kim YH, Chae BC, Kim HJ, Kim KH, Kang KK, Ko BS, Lee MY (2006) Genetic analysis of polygonati rhizoma and polygonati odorati rhizoma using random amplified microsatlite polymorphism. Korean J Medicinal Crop Sci. 14:125-129
  2. Bao W, Pan H, Lu M, Ni Y, Zhang R, Gong X (2007) The apoptotic effect of sarsasapogenin from Anemarrhena asphodeloides on HepG2 human hepatoma cells. Cell Biol Int. 31:887-892 https://doi.org/10.1016/j.cellbi.2007.02.001
  3. Bae KH (2000) The medicinal plants of Korea. Kyohaksa. Seoul. Korea. p. 546
  4. Chase MW, De Bruijn AY, Cox AV, Reeves G, Rudall PJ, Johnson MAT, Eguiarte LE (2000) Phylogenetics of Asphodelaceae (Asparagales): An analysis of plastid rbcL and trnL-F DNA sequences. Ann Bot-London. 86:935-951 https://doi.org/10.1006/anbo.2000.1262
  5. Chung KF (2002) Over-the-counter sleeping pills: a survey of use in Hong Kong and a review of their constituents. Gen Hosp Psychiat. 24:430-435 https://doi.org/10.1016/S0163-8343(02)00210-4
  6. Han SH, Park SI (1997) Classification of Korean native Anemarrhena asphodeloides Bunge by cluster analysis. Korean J Medicinal Crop Sci. 5:266-275
  7. Hoa NK, Phan DV, Thuan ND, Ostenson CG (2004) Insulin secretion is stimulated by ethanol extract of Anemarrhena asphodeloides in isolated islet of healthy Wistar and diabetic Goto-Kakizaki rats. Exp Clin Endocr Diab. 112:520-525 https://doi.org/10.1055/s-2004-821309
  8. Hu Y, Xia Z, Sun Q, Orsi A, Ree D (2005) A new approach to the pharmacological regulation of memory: sarsasapogenin improves memory by elevating the low muscarinic acetylcholine receptor density in brains of memory-deficit rat models. Brain Res. 1060:26-39 https://doi.org/10.1016/j.brainres.2005.08.019
  9. Kimura I, Sugihara N, Nakashima Y, Chen FJ, Kimura M (1999) The anti-hyperglycaemic blend effect of traditional Chinese medicine Byakko-ka-ninjin-to on alloxan and diabetic KK-Cay mice. Phytother Res. 13:484-488 https://doi.org/10.1002/(SICI)1099-1573(199909)13:6<484::AID-PTR485>3.0.CO;2-X
  10. Kim SY, Choi HW, Bang JW (2004) Physical mapping of rDNAs using McFISH in Anemarrhena asphodeloides Bunge. Korean J Medicinal Crop Sci. 12:515-518
  11. Kim SY, Koo DH, Bang JW (2002) Karyotype analysis of Anemarrhena asphodeloides Bunge. Korean J Medicinal Crop Sci. 10:144-146
  12. Lai L, Lin LC, Lin JH, Tsai TH (2003) Pharmacokinetic study of free mangiferin in rats by microdialysis coupled with microbore high-performance liquid chromatography and tandem mass spectrometry. J Chromatogr A. 987:367-374 https://doi.org/10.1016/S0021-9673(02)01415-2
  13. Lang P, Dane F, Kubisiak TL (2006) Phylogeny of Castanea (Fagaceae) based on chloroplast trnT-L-F sequence data. Tree Genetics & Genomes. 2:132-139 https://doi.org/10.1007/s11295-006-0036-2
  14. Li TJ, Qiu Y, Yang PY, Rui YC, Chen WS (2007) Timosaponin B-II improves memory and learning dysfunction induced by cerebral ischemia in rats. Neurosci Lett. 421:147-151 https://doi.org/10.1016/j.neulet.2007.04.082
  15. Miura T, Ichiki H, Iwamoto N, Kato M, Kubo M, Sasaki H, et al (2001) Anti-diabetic activity of the rhizoma of Anemarrhena asphodeloidesand active components, mangiferin and its glucoside. Biol Pharm Bull. 24:1009-1011 https://doi.org/10.1248/bpb.24.1009
  16. Pan Y, Liang Y, Wang H, Liang M (2004) Antioxidant activities of several Chinese medicine herbs. Food Chem. 88:347-350 https://doi.org/10.1016/j.foodchem.2004.02.002
  17. Park HJ, Lee JY, Moon SS, Hwang BK (2003) Isolation and anti-oomycete activity of nyasol from Anemarrhena asphodeloides rhizomes. Phytochemistry. 64:997-1001 https://doi.org/10.1016/S0031-9422(03)00462-X
  18. Shoemaker M, Hamilton B, Dairkee SH, Cohen I, Campbell MJ (2005) In vitro anticancer activity of twelve Chinese medicinal herbs. Phytother Res. 19:649-651 https://doi.org/10.1002/ptr.1702
  19. Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding region of chloroplast DNA. Plant Mol Biol. 17:1105-1109 https://doi.org/10.1007/BF00037152
  20. Tohda C, Sugahara H, Kuraishi Y, Komatsu K (2000) Inhibitory effect of Byakko-ka-ninjin-to on itch in a mouse model of atopic dermatitis. Phytother Res. 14:192-194 https://doi.org/10.1002/(SICI)1099-1573(200005)14:3<192::AID-PTR609>3.0.CO;2-F
  21. Wang GJ, Lin LC, Chen CF, Cheng JS, Lo YK, Chou KJ, Lee KC, Liu CP, Wu YY, Su W, Chen WC, Jan CR (2002) Effect of timosaponin A-III, from Anemarrhena asphodeloides Bunge (Liliaceae), on calcium mobilization in vascular endothelial and smooth muscle cells and on vascular tension. Life Sci. 71: 1081-1090 https://doi.org/10.1016/S0024-3205(02)01794-0
  22. Yeum HS, Lee YC, Kim SH, Roh SS, Lee JC, Seo YB (2007) Fritillaria cirrhosa, Anemarrhena asphodeloides, Lee-Mo-Tang and cyclosporine A inhibit ovalbumin-induced eosinophil accumulation and Th2-mediated bronchial hyper-responsiveness in a murine model of asthma. Journal compilation. 100:205-213
  23. Yun JS, Kim IH, Park JS, Lee CH, Hong EY, Yun T, Jong SK (2006) Characterization of 18S rDNA in Polygonatum sp. collections. Korean J Medicinal Crop Sci. 14:178-182
  24. Zhang J, Meng Z, Zhang M, Ma D, Xu S, Kodama H (1999) Effect of six steroidal saponins isolated from Anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood. Clin Chim Acta. 289:79-88 https://doi.org/10.1016/S0009-8981(99)00160-6
  25. Zhang J, Zhang M, Sugahara K, Sagara Y, Meng Z, Xu S, Kodama H (1999) Effect of steroidal saponins of Anemarrhenae rhizoma on superoxide generation in human neutrophils. Biochem Bioph Res Co. 259:636-639 https://doi.org/10.1006/bbrc.1999.0831