Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Characterization of a Marine Derived Bacterium Glaciecola sp. SL-12 Producing β-agarase

한천분해효소를 생산하는 해양유래 세균 Glaciecola sp. SL-12의 분리 및 특성

  • Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Ok-Hee (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Jang, Hyo-Jung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Jang, Min-Kyung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Yoo, Ki-Hwan (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
  • 이동근 (신라대학교 의생명과학대학 제약공학과) ;
  • 이옥희 (신라대학교 의생명과학대학 제약공학과) ;
  • 장효정 (신라대학교 의생명과학대학 제약공학과) ;
  • 장민경 (신라대학교 의생명과학대학 제약공학과) ;
  • 유기환 (신라대학교 의생명과학대학 제약공학과) ;
  • 이상현 (신라대학교 의생명과학대학 제약공학과)
  • Published : 2008.01.31
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Abstract

A novel agar-degrading bacterium SL-12 was isolated from seashore of Kijang at Busan, Korea, and cultured in marine broth 2216 media. Isolated bacterium SL-12 was identified as Glaciecola genus by 16S rDNA sequencing with 98% identity. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was $30^{\circ}C$. The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is ${\beta}$-agarase. Thus, isolated bacterium and the enzyme would be useful for the industrial production of neoagarobiose.

기능성 한천올리고당 생산에 사용할 수 있는 유전자원을 확보하기 위하여 동해안 기장 해수에서 한천분해활성을 보이는 해양유래 세균 SL-12를 분리하였으며 165 rDNA염기서열 분석으로 해양기원의 Glaciecola 속과 가장 유사한 균주임을 확인하였다. SL-12 균주가 생성하는 한천분해효소(agarase)의 최적 pH는 pH 7.0 (20 mM sodium phosphate 완충용액)이고 최적 온도는 $30^{\circ}C$로 나타났다. 분리 된 Glaciecola sp. SL-12 균주가 생산하는 한천분해효소의 분해산물에 대한 TLC 분석결과, 기능성 한천올리고당을 생산하는 ${\beta}$-agarase로 판명되어 산업적 활용 가능성이 높은 것으로 기대된다.

Keywords

References

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