생명과학회지 (Journal of Life Science)

  • 제18권1호
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  • Pages.23-29
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  • 2008
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Mesangial 세포에서 고포도당에 의해 유도되는 insulin-like growth factor 분비 촉진작용에 대한 ginsenosides의 차단 효과

Ginsenosides Protect the High Glucose-induced Stimulation of IGFs in Mesangial Cells

  • 배춘식 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 임도선 (을지대학교 보건과학대학 치위생학과) ;
  • 윤병철 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 정문진 (조선대학교 치과대학 구강조직학교실) ;
  • 윤경철 (전남대학교 의과대학 안과학교실) ;
  • 박수현 (전남대학교 기능성 식품센터)
  • Bae, Chun-Sik (College of Veterinary Medicine, Biotechnology Research Institute,Chonnam National University) ;
  • Lim, Do-Seon (Department of Dental Hygiene, Eulji University) ;
  • Yoon, Byeong-Cheol (College of Veterinary Medicine, Biotechnology Research Institute,Chonnam National University ) ;
  • Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Yoon, Kyung-Chul (Department of Ophthalmology, Chonnam National University, Medical School and Hospital) ;
  • Park, Soo-Hyun (Functional Food Center, Chonnam National University)
  • 발행 : 2008.01.31
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초록

인삼은 고전적으로 항당뇨 효과가 있는 것으로 보고되고 있다. Insulin-like growth factor (IGF) 역시 당뇨병성 신증의 발병 초기에 중요한 역할을 하는 것으로 알려져 있다. 이에 본 연구에서는 mesangial 세포에서 고포도당에 의한 IGF 분비에 대한 ginsenoside의 차단 효과 및 이와 관련된 신호전달계를 알아보았다. 결과는 다음과 같다. 고포도당에 의해 증가 되었던 IGF-I 및 IGF-II 분비 촉진 작용은 GTS, PD 및 PT 처리 시 차단되었으며, 세포 성장 촉진작용에서도 같은 효과를 볼 수 있었다. 아울러 고포도당에 의한 산화성 스트레스 종가, GSH 감소, AA 방출 증가 작용 및 $PGE_2$ 합성 증가 작용은 GTS 처리시 현저하게 차단되었으며 PD 및 PT 처리 시 역시 억제 되는 것으로 나타났다. 이상의 결과를 볼 때 mesangial 세포에서 ginsenoside는 산화성 스트레스 및 arachidonic acid 활성 경로를 억제하여 고포도당에 의한 IGFs 분비 작용을 차단하는 것으로 나타났다.

Panax ginseng C. A. MEYER is one of the most widely used herbal medicines in the Asian countries and has diverse functions including anti-diabetic action. The dysfunctions of mesangial cells in hyperglycemic conditions are implicated in the development of diabetic nephropathy. Insulin-like growth factors (IGFs) are also associated with the onset of diabetic nephropathy. Thus, we examined the effect of ginsenosides against high glucose-induced dysfunction of primary cultured rat mesangial cells. In the present study, high glucose increased IGF-I and IGF-II secretion in mesangial cells. Ginsenoside total saponin (GTS) prevented high glucose-induced increase of IGF-I and IGF-II secretion in mesangial cells. In addition, GTS prevented high glucose-induced increase of lipid peroxide formation and decrease of GSH contents. GTS also ameliorates high glucose-induced increase of arachidonic acid release and decrease of prostaglandin $E_2$. In conclusion, GTS prevented high glucose-induced dysfunction of mesangial cells via inhibition of oxidative stress and arachidonic acid pathways.

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