Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibitory Effects of Green Tea against Squalene Synthase

녹차의 squalene synthase 저해효과

  • Choi, Sung-Won (Department of Food and Culinary Art, Osan College) ;
  • Hur, Nam-Yoon (Department of Food and Culinary Art, Osan College) ;
  • Lee, Han-Seung (Department of Bio-Food Materials, College of Medical and Life Sciences, Silla University) ;
  • Baik, Moo-Yeol (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Lee, Jeong-Gyu (Department of Family Medicine, Pusan National University School of Medicine)
  • 최성원 (오산대학교 호텔조리계열) ;
  • 허남윤 (오산대학교 호텔조리계열) ;
  • 이한승 (신라대학교 바이오식품소재학과) ;
  • 백무열 (경희대학교 식품공학과) ;
  • 안순철 (부산대학교 의과대학 미생물학교실) ;
  • 이정규 (부산대학교 의과대학 가정의학과)
  • Published : 2008.02.28
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Abstract

Various biological resources from plants, animals, mushrooms, microorganisms, and foods were tested for the inhibitory activity against squalene synthase (SQS). Among 32 samples, more than one fourths (9 samples) exhibited significant SQS inhibitory activity. Interestingly, SQS inhibitory activity was detected in the samples such as green tea, fermented soybean paste, and plum juice. The SQS inhibitory activity of green tea was not only high but also stable. Its SQS inhibitors were supposed to be catechin derivatives, which have been known to be main bioactive components in green tea. The galloyl catechins showed higher SQS inhibitory activity compared to the nongalloyl catechins. Especially, (-)-epigallocatechin gallate appeared to be strongest inhibitor against squalene synthase ($IC_{50}=90{\mu}M$).

콜레스테롤 생합성 과정에 있어서 속도조절 단계 효소의 하나인 squalene synthase에 대한 저해물질의 탐색을 목적으로, 30종의 다양한 천연물을 대상으로 squalene synthase에 대해 저해효과를 검토한 결과 녹차추출물에서 비교적 저해활성이 높고 재현성이 있게 저해효과를 나타내는 것으로 확인되었다. 녹차에 함유되어 있는 squalene synaase에 대한 저해물질의 용매추출성을 검토한 결과 ethyl acetate와 n-butanol 층에 저해물질이 많이 함유되어 있는 것으로 확인되었으며 저해물질은 녹차의 polyphenol 화합물인 catechin에 의한 것으로 추정되었다. Catechin 표준용액의 각 농도에 따른 squalene synthase 저해작용을 살펴 본 결과, (-)-epigallocatechin gallate, (-)-epicatechin gallate, (-)-epigauocatechin, (-)-epicatechin, (+)-catechin의 순으로 저해활성이 강한 것으로 나타났으며 가장 강한 저해활성을 나타내는 (-)-epigallocatechin gallate의 $IC_{50}$값은 $90{\mu}M$이었다.

Keywords

References

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