DOI QR코드

DOI QR Code

고지방식이 섭식 횐쥐의 간조직에 미치는 한방초콩환의 영향

Effect of Herbal Chokong Pill on the Microstructure of Liver Cell in Rats Fed High Fat Diet

  • 박찬성 (대구한의대학교 한방식품조리영양학부) ;
  • 김동한 (대구한의대학교 한방식품조리영양학부) ;
  • 김미림 (대구한의대학교 한방식품조리영양학부)
  • Park, Chan-Sung (Faculty of Herbal Food Cuisine & Nutrition, Daegu Haany University) ;
  • Kim, Dong-Han (Faculty of Herbal Food Cuisine & Nutrition, Daegu Haany University) ;
  • Kim, Mi-Lim (Faculty of Herbal Food Cuisine & Nutrition, Daegu Haany University)
  • 발행 : 2008.02.28

초록

본 연구는 한방초콩환의 고지방식이를 섭식한 흰쥐의 간조직 지방제거 효과를 관찰하기 위하여 서목태(Rhynchosia nulubilis)를 식초에 15일간 절인 후, 5종류의 한약재(감초, 산조인, 백출, 황기, 산수유)를 첨가하여 한방초콩환을 제조하고, 고지방식이로 흰쥐의 비만을 유도하면서 고지방식이에 한방초콩환을 1%와 5% 첨가하여 9주 동안 투여한 후 간장의 무게, 혈청 효소활성도 및 간세포의 미세구조를 투과형전자현미경(TEM)으로 관찰하였다. HF군은 정상군의 간 무게에 비하여 약 35% 증가된 반면에 T5군의 경우는 HF군에 비하여 약 13% 감소하였다. 혈청 중 GOT 활성도는 정상군과 HF군이 비슷한 수준이었으나 T1군과 T5군에서는 HF군에 비하여 각각, 6.1%, 17.8% 감소하였으며 혈청 중 GPT 활성도 역시 정상군과 HF군이 거의 비슷한 수준이었으나 T1군과 T5군에서는 HF군에 비하여 각각 25.4, 32.4% 감소하였다. 간세포조직의 지방축적 정도 및 형태적 특징을 전자현미경으로 관찰한 결과, 정상군의 모양은 원형 내지 다각형을 나타내고 핵은 크고 둥글며 세포의 중앙에 위치해 핵질이 고르게 발달해 있을 뿐만 아니라 세포질에는 미토콘드리아를 비롯한 각종 세포 소기관이 고루 분포되어 있었으며 세포 전반에 걸쳐 지방구는 보이지 않았다. HF군은 세포전반에 걸쳐 지방구에 뒤덮여 세포의 형태 관찰이 불가능할 정도였으며 지방의 과잉 축적으로 인한 전자밀도가 낮아 백색을 나타내어 세포소기관의 막과 형태가 불분명한 지방간의 심각한 양상을 볼 수 있었다. T1군은 HF군에 비하여 지방구가 많이 소멸되었으며, T5군은 T1군에 비하여 지방세포의 크기와 수가 현저히 줄어든 것이 관찰 되었고, 더욱더 선명한 핵과 핵막 및 세포질의 전자밀도가 높아 지방질의 감소를 볼 수 있었다.

The purpose of this study is to investigate the effects of the herbal Chokong pill (hereafter HCKP) on the liver cell and enzyme activities of serum in rat. HCKP were mixed with pickled black soybeans and five different kinds of medicinal herbs (Rhynchosia nulubilis, Glycyrrhiza uralensis, Zizyphus vulgaris, Atractylodes macrocephala K., Astragalus membranaceus and Cornus officinalis). Four groups of male Sprague-Dawley rats were fed by different diets for 9 weeks: normal diet (Nor), high-fat diet (HF), high-fat diet supplemented with 1% (T1) and 5% (T5) HCKP powder, respectively. Depending on the presence of HCKP in high fat diet, the activities of the blood serum GOT and GPT were decreased. GOT and GPT activities of T1 and T5 were decreased 6.1%, 17.8% and 25.4%, 32.4% compared with HF. On microstructure observing through the transmission electron microscope (TEM) of liver cell, in normal group, a normal large and clear nucleus, rough endoplasmic reticulum (RER) and mitochondria possessing well-defined double outer-limiting membranes were found. However, in HF, it was hard to observe the microstructures in cytoplasm, because of too many fat granules. It showed severely damaged cell, pyknotic nucleus, swollen disintegrating RER and mitochontria loosing the cristae. In T1, there were more repaired liver cells and less fat granules than HF. In T5, there were much less numbers and smaller size of the fat granules than T1, and the morphology was similar to normal cell.

키워드

참고문헌

  1. Bae, E. A. and G. S. Moon. 2003. A study on the antioxidative activities of Korean soybeans. Korean J. Food Sci. Nutr. 26, 203-208
  2. Bron, D. and R. Asmis. 2001. Vitamin B and the prevention of atherosclerosis. Int. J. Vitam. Nutr. Res. 71, 18-24 https://doi.org/10.1024/0300-9831.71.1.18
  3. Caragay, A. B. 1992. Cancer-preventive foods and ingredients. Food Technol. 46, 65-78
  4. Carroll, K. K., P. M. Giovannetti, M. W. Huff, O. Moase, D. C. K. Roberts and B. M. Wolfe. 1978. Hypocholesterolemic effect of substituting soybean protein for animal protein in the diet of healthy young men. Am. J. Clin. Nutr. 31, 1312-1314 https://doi.org/10.1093/ajcn/31.8.1312
  5. Choi, Y. S. and S. Y. Lee. 1993. Cholesterol-lowering effects of soybean products (curd or curd residue) in rats. J. Korean Soc. Food Nutr. 26, 673-677
  6. Han, J. S., H. D. Hong and S. R. Kim. 2007. Changes in isoflavone content and mass balance during soybean processing. Food Sci. and Biotechnol. 16, 426-433
  7. Kang, M. J., W. Oh, H. K. Lee, H. S. Chung, S. M. Lee, C. Kim, H. J. Lee, D. W. Yoon. 2000. Anti-obesity effect of PM-F2-OB, an anti-obesity herbal formulation, on rats fed a high-fat diet. Biol. Pharm. Bull. 27, 1251-1256 https://doi.org/10.1248/bpb.27.1251
  8. Kim, H. S., G. J. Kim, H. S. Kim. 1998. Effect of the Feeding Platycodon grandiflorum on Lipid Components of Liver and Liver Function in Hypercholesterolemia Rats. Kor. J. Food and Nutr. 11, 312-318
  9. Kim, T. S. 1996, The Plant of Korea, Seoul National Univ. Publish, Seoul
  10. Kim, Y. S., S. H. Byeon, B. l. Seo, S. C. Kim, M. Gug, E. H. Jo. 2000. The Oriental medicines effects on cure and prevention of an obesty (2). Kor. J. Herbology 15, 73-82
  11. Kim, Y. S., S. H. Byun, S. C. Kim, M. Kuk, E. H. Cho. 2001. Effect on cure and prevention of the obesity (VIII). Kor. J. Herbology 16, 41-48
  12. Lee, E., W. J. Kim, Y. J. Lee, M. K. Lee, P. G. Kim, Y. J. Park, S. K. Kim. 2003. Effects of natural complex food on specific enzymes of serum and liver and liver microstructure of rats fed a high fat diet. Kor. Soc. Food Sci. Nutr. 32, 256-262 https://doi.org/10.3746/jkfn.2003.32.2.256
  13. Lee, J. S., M. K. Lee, T. Y. Ha, S. H. Bok, H. M. Park, K. S. Jeong, M. N. Woo, G. M. Do, J. Y. Yeo, M. S. Choi. 2006. Supplementation of whole persimmon leaf improves lipid profiles and suppresses body weight gain in rats fed high-fat diet. Food Chem. Tox. 44, 1875-1883 https://doi.org/10.1016/j.fct.2006.06.014
  14. Lin, R. I. S. 1994. In Fuinctional Foods: Phytochemicals and antioxidants. Chapman & Hall, New York
  15. Messina, M., V. Persk, K. D. R. Setchell and S. Branes. 1994. Soy intake and cancer risk: A review of the in vitro and in vivo data. Nutr. Cancer 21, 113-131 https://doi.org/10.1080/01635589409514310
  16. Ministry of Health and welfare. 2000. 1998 Yearbook of health and nutrition statistics
  17. Moon, S. P., J. B. Koh. 2004. Effects of liquid culture of Coriolus Versicolor on lipid metabolism and enzyme activities in rats fed high fat diet. Kor. Nutr. Soc. 37, 88-94
  18. Oh, C. H., E. J. Kim, K. H. Lee, M. K. Moon, M. G. Cho, J. H. Kim, S. H. Oh, T. K. Lee, T. Y. Shin, D. K. Kim. 2006. Antiproliferative constituents from vinegar treated small black soybean (Glycine max Merr). Natural Product Sci. 12, 109-112
  19. Park, C. S., D. H. Kim, M. L. Kim, J. M. Suk, M. R. Kim. 2007. Effects of mixed pills of Chokong (pickled black soybeans) with medicinal herbs on body weight gain and lipid profiles in rats fed high-fat diet. Kor. J. Herbology. 22, 127-135
  20. Park, M. S. C., B. J. Kudchodkar and G. U. Liepa. 1987. Effect of dietary animal and plants proteins on the cholesterol metablolism in immature and mature rats. J. Nutr. 117, 30-35 https://doi.org/10.1093/jn/117.1.30
  21. Shin, M. K. and S. H. Han. 2006. Effect of Lotus (Nelumbo nucifera Gaertner) leaf powder on lipid concentrations in rats fed high fat diet rats. Kor. J. Food Culture 21, 202-208
  22. Shin, M. K. and S. H. Han. 2002. Effects of methanol extracts from Rhynchosia nulubilis on serum lipid concentrations in rats fed high fat and high cholesterol diet. Kor. J. dietary Culture 17, 64-69
  23. Statistics office. 1999. 1998 Yearbook death cause statistics
  24. Terpastra, A. H. M., G. Tintelen and C. E. West. 1982. The hypocholesterolemic effect of dietary soy protein in rats. J. Nutr. 11, 810-817