Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibition of Phorbol 12-myristate-13-acetate Induced Cyclooxygenase-2 Activity by Three-step Fermented Soybeans

PMA에 의해 유도된 cycooxygenase-2 활성에 대한 새로운 발효법에 의한 대두산물의 억제 효능

  • Park, Cheol (Department of Biochemistry, Dongeui University College of Oriental Medicine) ;
  • Lee, Jeong-Ok (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Ryu, Chung-Ho (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine)
  • 박철 (동의대학교 한의과대학 생화학교실 및 대학원 바이오물질제어학과(BK21)) ;
  • 이정옥 (경상대학교 농업생명과학대학 응용생명공학과) ;
  • 류충호 (경상대학교 농업생명과학대학 응용생명공학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실 및 대학원 바이오물질제어학과(BK21))
  • Published : 2008.02.28
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Abstract

In this study, we examined the effects of the fermented soybeans by Bacillus subtilis (FSB) and the novel three-step fermented soybeans (TFS) on the expression and activity of COX-2 in human leukemic U937 cell model. Treatment of phorbol 12-myristate 13-acetate (PMA) significantly induced pro-inflammatory mediators such as COX-2 expression and prostaglandin $E_2\;(PGE_2)$ production, whereas the levels of COX-1 remained unchanged. However, pre-treatment with FSB and TFS significantly attenuated the PMA-induced COX-2 protein as well as mRNA, which was associated with inhibition of $PGE_2$ production. Moreover, TFS exerts a much better inhibitory activity than FSB against PMA-induced activation of COX-2 and production of $PGE_2$ in U937 cells. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-inflammatory activity of FSB and TFS.

이상의 연구 결과에 의하면 U937 세포에서 FSB 및 TFS 모두 2 mg/ml의 농도에서 6시간까지는 세포증식에 아무런 영향을 미치지 못하였고 PMA 처리에 의해서도 세포증식에는 변화가 없었으며, 세포의 형태 및 핵의 형태도 PMA와 FSB 및 TFS의 처리에 의해서 아무런 변화가 나타나지 않았다. 그러나 COX-2의 발현의 정도는 PMA 처리에 의해서 증가하였고, 이렇게 증가한 COX-2는 FSB 및 TFS의 선처리에 의해서 효과적으로 억제되는 것으로 나타났다 또한 COX-2에 의해 생성되어 염증반응을 유발하며 세포분열이나 증식에 영향을 줌으로서 각종 질병의 유발과 진행에 중요한 역할을 하는 것으로 알려져 있는 $PGE_2$의 경우도 PMA처리에 의하여 증가하였으며 FSB 및 TFS 처리에 의해서 강하게 억제되었다. 특히 FSB에 비하여 TFS를 선처리하였을 경우 PMA에 의하여 과발현된 COX-2 및 $PGE_2$ 생성의 억제정도가 더 강하게 나타났다. 이러한 결과는 FSB 및 TFS의 항염증기전 해석을 위한 이해와 향후 지속적인 연구를 위한 귀중한 자료가 될 것으로 사료된다.

Keywords

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