동서간호학연구지 (Journal of East-West Nursing Research)
- 제14권2호
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- Pages.74-80
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- 2008
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- 1226-4938(pISSN)
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- 2671-8448(eISSN)
한약재에서의 멜라닌 생성 억제 효과 검색
Screening of Inhibitory Effects of an Oriental Herb on Melanogenesis
-
강경아
(경희대학교 간호과학대학.동서간호학연구소)
;
- 한상숙 (경희대학교 간호과학대학.동서간호학연구소) ;
-
이무형
(경희대학교 의과대학.고황의학연구소)
;
-
김연정
(경희대학교 간호과학대학.동서간호학연구소)
-
Kang, Kyoung-Ah
(College of Nursing Science, East-west Nursing Institute, Kyung Hee University)
;
- Han, Sang-Sook (College of Nursing Science, East-west Nursing Institute, Kyung Hee University) ;
-
Lee, Mu-Hyoung
(College of Medicine, Kowhang Medical Institute, Kyung Hee University)
;
-
Kim, Youn-Jung
(College of Nursing Science, East-west Nursing Institute, Kyung Hee University)
- 발행 : 2008.12.31
초록
Purpose: To screen candidate oriental herb material for antimelanogenics. Methods: Oriental herbs (n=100) were screened for mushroom tyrosinase inhibitory activity in vitro using the HM3KO human melanin cell line cultured in DMEM supplemented with 10% fetal bovine serum. Cytotoxicity was assessed by a cell viability assay involving 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Trypan Blue exclusion, and cell enumeration. Results: Tyrosinase inhibitory effects on 100 oriental herbs was evident. Of these, 11 herbs inhibited tyrosinase activity by 40% without being cytotoxic to HM3KO cells. Three herb varieties significantly decreased melanin synthesis in HM3KO cells. Conclusions: Oriental herb can have antimelanogenic effects indicating their potential for functional therapeutic use in dermatological whitening.