Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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The Effects of Human Adipose Tissue-derived Stem Cells on Degenerative Change of Knee in Rabbit Model

가토 모델에서 인체지방유래 줄기세포가 슬관절의 퇴행성 변화에 미치는 영향

  • Jeong, Ki-Hwan (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University) ;
  • Kim, Seok-Kwun (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University) ;
  • Jeong, Jae-Oo (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University) ;
  • Heo, Jeong (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University) ;
  • Kwon, Yong-Seok (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University) ;
  • Lee, Keun-Cheol (Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University)
  • 정기환 (동아대학교 의과대학 성형외과학교실) ;
  • 김석권 (동아대학교 의과대학 성형외과학교실) ;
  • 정재우 (동아대학교 의과대학 성형외과학교실) ;
  • 허정 (동아대학교 의과대학 성형외과학교실) ;
  • 권용석 (동아대학교 의과대학 성형외과학교실) ;
  • 이근철 (동아대학교 의과대학 성형외과학교실)
  • Received : 2008.01.24
  • Accepted : 2008.09.03
  • Published : 2008.11.10
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Abstract

Purpose: The survival of bone marrow derived stem cell was reported several times. But the survival of adipose tissue derived stem cells(hASCs) was not mentioned on. We studied the adipose tissue derived stem cell's survival and effect on articular cartilage in rabbits. Methods: Osteoarthritis was induced in twenty New Zealand white rabbits by intraarticular injection of monosodium iodoacetate(MIA). After four weeks, hASCs were also injected into the knee joints space without any vehicle, but the control group received phosphate buffered saline only. The histologic grade of articular cartilage was measured in 4 and 8 weeks after the transplantation of hASC and the viability of injected stem cells measured by Fluorescent in situ Hybridization (FISH) examination. Results: After 4 and 8 weeks from hASCs transplantation, histologic grade was not significantly difference between two groups(p>0.05), and the Y chromosome of the transplanted hASCs was not detected in articular cartilage. Conclusion: We found that direct injection of hASC in joint space didn't work on damaged articular cartilage repair.

Keywords

References

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