한국어병학회지 (Journal of fish pathology)

  • 제21권2호
  • /
  • Pages.81-91
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  • 2008
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

Vibrio harveyi에서 분리한 extracellular products (ECPs)의 특성과 독성

Characterization and pathogenicity of extracellular products (ECPs) of Vibrio harveyi

  • 최정현 (국립수산과학원 동해특성화연구센터) ;
  • 원경미 (부경대학교 수산과학연구소) ;
  • 하수진 (부경대학교 수산생명의학과) ;
  • 김이청 (국립수산과학원 동해특성화연구센터) ;
  • 변순규 (국립수산과학원 동해특성화연구센터) ;
  • 이배익 (국립수산과학원 동해특성화연구센터) ;
  • 이종하 (국립수산과학원 동해수산연구소) ;
  • 허민도 (부경대학교 수산생명의학과) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Choi, Jeong-Hyun (East Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Won, Kyoung-Mi (Institute of Fisheries Science, Pukyong National University) ;
  • Ha, Su-Jin (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Yi-Cheong (East Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Byun, Soon-Gyu (East Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Lee, Bae-Ik (East Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Lee, Jong-Ha (East sea Fisheries Research Institute, NFRDI) ;
  • Huh, Min-Do (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Park, Su-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 발행 : 20080800
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초록

Vibrio harveyi, one of the major causal agent of vibriosis, affects a diverse range of marine vertebrates and invertebrates over a wide geographical area. The aims of this study were to investigate the characteristics of extracellular products (ECPs) from an isolate of the pathogenic non‐luminous V. harveyi and the luminous V. harveyi. And ECPs of V. harveyi were examined the pathogenicity to the black rockfish, Sebastes schlegeli, and histopathological traits of internal organs injected by ECPs. Four strains of V. harveyi cultures produced ECPs showing various enzymatic activites (caseinase, gelatinase, phospholipase, lipase, haemolysin). The ECPs showed strong cytotoxicity on macrophages of black rockfish, Sebastes schlegeli and olive flounder, Paralichthys olivaceus. Especially, the virulence of ECPs from the isolate of V. harveyi FR 2 was higher in the intraperitoneally injected black rockfish. Also, the ECPs of V. harveyi FR 2 caused the expansion of sinusoids in the liver, the activation of ellipsoid in the spleen and the sloughing of the epidermal cell in the intestine. It was suggested that the ECPs from V. harveyi play an important role in the pathogenicity process of the V. harveyi.

키워드

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