Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Tributyltin chloride (TBTCl) toxicity on the survival rate and burrowing activity of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae)

대복, Gomphina veneriformis의 생존 및 저질 잠입성에 미치는 TBTCl의 독성

  • Park, Jung-Jun (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Jin, Young-Guk (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Lee, Jung-Sick (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
  • 박정준 (전남대학교 수산해양대학 수산생명의학과) ;
  • 진영국 (전남대학교 수산해양대학 수산생명의학과) ;
  • 이정식 (전남대학교 수산해양대학 수산생명의학과)
  • Published : 20080400
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to find out biological responses of bivalves exposed to organotin compound.The results of the study confirmed that tribultyltin chloride (TBTCl) induce reduction of survival rate andburrowing activity, and histopathological feature in the foot structure of the equilateral venus, Gomphinaveneriformis. The experimental period was 36 weeks. The experimental groups consisted of a control and 3TBTCl exposure groups (0.4, 0.6, 0.8 ym TBTCl L'). The survival rate and burrowing activity were record-ed daily. For histological analysis, foot tissues were fixed in Bouin' s fluid and then stained H-E stain, AB-PAS (PH 2.5) reaction and Masson's trichrome stain after having serially sectioned the tissue by paraffinmethod at thickness of 4-6 ym. The survival rate was not significantly different between the control andexposure groups for 20 weeks, but in 0.8 Um TBTCl L', it was on the decreased ever since the exposure. Theburrowing activity was not significantly different in the exposure group compared to the control up to 12weeks, but in 0.6 and 0.8 ym TBTCl L', it measured the lowest level after 20 weeks. The foot is composedof the epidermal layer, connective tissue, and muscular layer. The epidermal layer is composed of simplecolumnar, cuboidal epithelia and mucous cells. The cilia were well developed on the apical surface ofepithelium, Circular, longitudinal and transverse muscle bundle were well developed in the muscular layer.The majority mucous cells showed blue color (542c) when it subjected to AB-PAS (PH 2.5) reaction. Nohistopathological alterations in the foot were observed up to 12 weeks. After 20 weeks of exposure to 0.8 (anTBTCl L'', the foot samples of exposed G. veneriformis showed disappearance of cilia and striated borderpartially and extension of hemolymph sinus. The mucous cell increased in the marginal of foot. At 28-weekof exposure to 0.4 ym TBTCl L', it observed weekly acid (564c), neutral (264c) and mixed mucous cell. At36-week of exposure to 0.6 ym TBTCl L', it showed fragmentation of the muscle and collagen fiber bundle,and also diappearance of cilia on epithelia and edema of epithelium in 0.8 ym TBTCl L''.

Keywords

References

  1. Abel, P.D.: Effects of some pollutants on the filtration rate of Mytilus edulis. Mar. Poll. Bull., 7: 228-231, 1976 https://doi.org/10.1016/0025-326X(76)90267-8
  2. Akberali, H.B., Wong, T.M. and Trueman, E.R.: Behavioral and siphonal tissue responses of Scrobicularia plana (Bivalvia) to zinc. Mar. Environ. Res., 5: 251-264, 1981 https://doi.org/10.1016/0141-1136(81)90009-X
  3. Ansell, A.D.: The functional morphology of the British species of veneracea (Eulamellibranchia). J. Mar. Biol. Ass. U.K., 41: 489-515, 1961 https://doi.org/10.1017/S0025315400024012
  4. Brand, A.R.: Heart action of the freshwater bivalve Anodonta anatian duting activity. J. Exp. Biol., 65: 685-698, 1976
  5. Byrne P.A. and O'Halloran, J.: The role of bivalve molluscs as tools in estuarine sediment toxity testing: a review. Hydrobiologia, 465: 209-217, 2001 https://doi.org/10.1023/A:1014584607501
  6. Drury R.A.B. and Wallington, E.A.: Carleton's histological technique. Oxford University Press, Oxford, pp. 520, 1980
  7. Dubey, S.K. and Roy, U.: Biodegradation of tributyltins (organotins) by marine bacteria. Appl. Organometal Chem., 17: 3-8, 2003 https://doi.org/10.1002/aoc.394
  8. Eble, A.F.: Biology of the hard clam. In: Anatomy and histology of Mercenaria mercenaria, vol. 6A, Kraeuter J.N. and Castagna, M. eds. Elsevier, New York, pp. 117-220, 2001
  9. Gregory, M.A., George, R.C., Marshall, D.J., Anandraj, A. and Mcclurg, T.P.: The effect of mercury exposure on the surface morphology of gill filaments in Perna perna (Mollusca: Bivalvia). Mar. Pollut. Bull., 39: 116-121, 1999 https://doi.org/10.1016/S0025-326X(99)00119-8
  10. Hebel, D.K., Jones, M.B. and Depledge, M.H.: Responses of crustaceans to contaminant exposure: a holistic approach. Estuar. Csttl. Shelf Sci., 44: 177-184, 1997 https://doi.org/10.1006/ecss.1996.0209
  11. Ju, S.M., Lee, J.W., Jin, Y.G., Yu, J. and Lee, J.S.: Effect of zinc bioaccumulation on survival rate, activity, growth and organ structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). J. Environ. Toxicol., 21: 115-126, 2006
  12. Kim, S.Y. and Lee, T.Y.: The effects of pollutants effluent from a steam-power plant on coastal bivalves. Ocean. Res., 10(1): 47-56, 1988
  13. Kim, C.K., Kim, D.H., Lee, J.S. and Lee, K.T.: Influence of heavy metals, ammonia and organotin compounds on the survival of arkshell clams, Scapharca broughtonii. Korean J. Malacol., 20: 93-105, 2004
  14. Matozzo, V., L. Ballarin, M.G. Marin.: Exposure of the clam Tapes philippinarum to 4- nonylphenol: changes in anti-oxidant enzyme activities and re-burrowing capability. Mar. Pollut. Bull., 48: 563-571, 2004 https://doi.org/10.1016/j.marpolbul.2004.01.011
  15. Morrison, C.M.: Histology and cell ultrastructure of the mantle and mantle lobes of the Estern oyster, Crassostrea virginica Gmelin: A summary atlas. Amer. Malac. Bull., 10(1): 1-24, 1993
  16. Otludil, B., Cengiz, E.I., Yildirim, M.Z. Unver, O. and Unlu, E.: The effects of endosulfan on the great ramshorn snail, Planorbariua corneus (Gastropoda, Pulmonata): a histopathological study. Chemosphere, 56: 707-716, 2004 https://doi.org/10.1016/j.chemosphere.2004.04.027
  17. Park, J.J. and Lee, J.S.: Cadmium toxicity on the survival rate and activity of the equilateral venus, Gomphina veneriformis. J. Kor. Fish. Soc., 36: 463-468, 2003
  18. Pekkarinen, M.: Scanning electron microscopy, whole-mount histology, and histochemistry of two Anodontine glochidia (Bivalvia: Unionidae). J. Zool., 74: 1964-1973, 1996
  19. Quinn, B., Gagne, F., Costello, M., McKenzie, C., Wilson, J. and Mothersill, C.: The endocrine disrupting effect of municipal effluent on the zebra mussel (Dreissena polymorpha). Aquat. Toxicol., 66: 297-292, 2004
  20. Regoli, F. and Orlando, E.: Accumulation and subcelluar distribution of metals (Cu, Fe, Mn, Pb and Zn) in the Mediterranean mussel, Mytilus galloprovincialis during a field transplant experiment. Mar. Pollut. Bull., 28: 592-600, 1994 https://doi.org/10.1016/0025-326X(94)90360-3
  21. Salazar, M.H. and Salazar, S.M.: Acute effects of (Bis) tributyltin oxide on marine organisms; Summary of work performed 1981-1983. Naval Ocean Systems Technical Report #1299, 1989
  22. Sunila, I.: Chronic histopathological effects of short-term copper and cadmium exposure on the gill of the mussels, Mytilus edulis. J. Invert. Pathol., 47: 125-142, 1986 https://doi.org/10.1016/0022-2011(86)90040-6
  23. Sunila, I.: Acute histopathological responses of the gill of the mussels, Mytilus edulis, to exposure by environmental pollutants. J. Invert. Pathol., 52: 137-141, 1988 https://doi.org/10.1016/0022-2011(88)90112-7
  24. Sunila, I. and Lindstrom, R.: Survival, growth and shell deformities of copper-and cadmiumexposed mussels, Mytilus edulis L. in Brackish water. Estuar. Coast. Shellf Sci., 21: 555-565, 1985 https://doi.org/10.1016/0272-7714(85)90056-3
  25. Thain, J.E.: Toxicity of TBT to bivalves: effects on reproduction, growth and survival. Oceans, 18: 1306-1313, 1986 https://doi.org/10.1109/OCEANS.1986.1160370
  26. Trueman, E.R., Brand, A.R. and Davis, P.: The dynamics of burrowing of some common littoral bivalves. J. Exp. Biol. 44: 469-492, 1966
  27. Valkirs, A.O., Davidson, B. and Seligman, P.F.: Sublethal growth effects and mortality from long-term exposure to tributyltin with marine bivalves and fish. Naval Ocean Systems Technical Report #1042, 1985
  28. Valkirs, A.O., Davidson, B. and Seligman, P.F.: Sublethal growth effects and mortality to marine bivalves from long-term exposure to tributyltin. Chemosphere, 16: 201-220, 1987 https://doi.org/10.1016/0045-6535(87)90124-X
  29. Watling H.R. and Watling, R.J.: Comparative effects of metals on the filtering rate of brown mussel, Perna perna. Bull. Environ. Contam. Toxicol., 29: 651-657, 1982 https://doi.org/10.1007/BF01606103
  30. Weis, J.S. and Perlmutter, J.: Effects of tributyltin on activity and burrowing behavior of the fiddler crab, Uca pugilator. Estuaries, 10: 342-346, 1987 https://doi.org/10.2307/1351893
  31. Yoo, J.S.: Korean shells in color. Iljisa Pub. Co., Seoul, pp. 196, 1976