Clinical and Experimental Pediatrics

대한소아청소년과학회 (The Korean Pediatric Society)
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34주 미만 미숙아 제대혈청 내 interleukin-12 농도와 영아기 천명 발생과의 관계

Relationship between cord blood level of IL-12 in preterm newborns and development of wheezing

  • 유효정 (이화여자대학교 의과대학 소아과학교실) ;
  • 박은애 (이화여자대학교 의과대학 소아과학교실) ;
  • 김지영 (이화여자대학교 의과대학 소아과학교실) ;
  • 조수진 (이화여자대학교 의과대학 소아과학교실) ;
  • 김영주 (이화여자대학교 의과대학 산부인과학교실) ;
  • 박혜숙 (이화여자대학교 의과대학 예방의학과학교실) ;
  • 하은희 (이화여자대학교 의과대학 예방의학과학교실)
  • Yu, Hyo Jung (Department of Pediatrics, Ewha Womans University, School of Medicine) ;
  • Park, Eun Ae (Department of Pediatrics, Ewha Womans University, School of Medicine) ;
  • Kim, Ji Young (Department of Pediatrics, Ewha Womans University, School of Medicine) ;
  • Cho, Soo Jin (Department of Pediatrics, Ewha Womans University, School of Medicine) ;
  • Kim, Young Ju (Department of Obstetrics and Gynecology, Ewha Womans University, School of Medicine) ;
  • Park, Hye Sook (Department of Preventive Medicine, Ewha Womans University, School of Medicine) ;
  • Ha, Eun Hee (Department of Preventive Medicine, Ewha Womans University, School of Medicine)
  • 투고 : 2008.01.07
  • 심사 : 2008.04.30
  • 발행 : 2008.07.15
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초록

목 적 : 알레르기 질환의 증가가 Th1/Th2 세포 면역의 불균형에 의한다는 것은 널리 받아들여지고 있다. 이에 본 연구에서는 미숙아의 제대혈에서 Th2 세포의 다른 사이토카인 분비를 증가시키는 IL-4, Th1 세포 분화를 억제하는 IL-10 및 강한 Th2 세포 억제 작용을 하는 IL-12를 측정하여 그 상관관계를 알아보고자 하였다. 방 법 : 2003년 1월부터 2005년 7월까지 이화여자대학교 부속 목동병원에서 출생한 34주 미만의 미숙아 중 보호자 동의하에 제대혈 검사가 가능하였던 46명을 대상으로 하여 의무기록을 통해 대상아의 천명의 발현 여부, 천식의 발생 여부에 대한 자료를 얻었다. 제대혈은 출생 시 채취하여, 즉시 혈청을 원심 분리하여 $-70^{\circ}C$ 냉동고에 보관하였다가 ELISA법을 이용하여 IL-4, IL-10 및 IL-12를 측정하였다. 결 과 : 관찰 기간은 12개월에서 36개월로 평균 16.0개월${\pm}13.2$일 이었고, 연구 기간 중 천명군은 18명이었으며, 비천명군과 비교하였을 때 IL-12가 유의하게 낮았다($366.60{\pm}140.40pg/mL$ vs. $435.10{\pm}91.20pg/mL$, P=0.009). IL-10은 천명군이 비천명군에 비해 높은 수치를 보였으나($2.60{\pm}5.11pg/mL$ vs $0.40{\pm}0.39pg/mL$) 통계적 의의는 없었다. IL-4는 차이가 없었다. 천식군은 4명으로 이들의 IL-12은 $282.60{\pm}128.88pg/mL$, 비천식 천명군 $392.40{\pm}138.06pg/mL$, 비천명군 $435.10{\pm}91.20pg/mL$로 세 군 사이에 유의한 차이가 있었다(P=0.045). 결 론 : 천명 및 천식이 발생한 영아에서 제대혈 IL-12 수치가 낮아 출생 시부터 면역학적인 불균형이 있었음을 예상할 수 있으며, 제대혈의 IL-12 수치로 생후 알레르기 호흡기 질환 발생을 예측하여 출생 시부터 보다 적극적인 알레르기 질환의 예방에 기여할 수 있을 것으로 생각된다.

Purpose : Abnormal activation patterns of Th1/Th2-cells have been suggested to increase the prevalence of allergic diseases. Prevention is regarded as an important corner stone in the management of allergic diseases. In this study, we have investigated the relationship between cord blood levels of IL-4, IL-10, and IL-12 in preterm newborns and the development of allergic respiratory diseases in infancy Methods : Forty-six preterm newborns born at the Ewha Womans University Mokdong Hospital between January 2003 and July 2005, were enrolled for this study, and consent was obtained to test their cord blood samples. Clinical history was obtained from the hospital records. Cord blood was obtained at birth and kept frozen until it was tested. The levels of IL-4, IL-10, and IL-12 were determined by enzyme-linked immunosorbent assay (ELISA). Results : All infants were followed-up for a median of $16.0months{\pm}13.2d$ (range, 12.0 to 36.0 months). Eighteen infants who developed wheezing showed lower cord blood levels of IL-12 ($366.60{\pm}140.40$ vs $435.09{\pm}91.20pg/mL$, P=0.009). Cord blood levels of IL-4 and IL-10 showed no significant difference between the two groups. Four newborns who later developed asthma, and infants with asthma showed lower IL-12 level in the cord blood than other groups. Conclusion : Lower concentration of cord blood levels of IL-12 in newborns who later developed wheezing and asthma suggested that they had abnormal activation patterns of Th1/Th2-cells at the time of birth, and cord blood IL-12 level can be used as a predictor of allergic respiratory diseases.

키워드

과제정보

연구 과제번호 : Development of Health-Related Technology for the Health of Mother and Child in Seoul Using a Birth Cohort Network

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피인용 문헌

  1. The Role of the Immune System in the use of Probiotic Lactic Acid Bacteria in Preventing and Treating Allergic Diseases vol.30, pp.1, 2008, https://doi.org/10.5851/kosfa.2010.30.1.1