Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Induction of Folate Sensitive Chromosomal Fragile Sites by Fudr in Pakistani Lohi Sheep (Ovis aries)

  • Ali, Ahmad (Department of Livestock Production, University of Veterinary and Animal Sciences) ;
  • Babar, Masroor Ellahi (Department of Livestock Production, University of Veterinary and Animal Sciences) ;
  • Abdullah, M. (Department of Livestock Production, University of Veterinary and Animal Sciences)
  • Received : 2007.08.27
  • Accepted : 2007.11.25
  • Published : 2008.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

An investigation to determine frequency and distribution of folate sensitive chromosomal fragile sites was carried out in a Pakistani breed of Lohi sheep to uncover fragile site phenomena. The means and standard errors of aberrant cell count (AC) and Number of aberrations (NoA) in Lohi sheep were $0.56{\pm}0.15$ and $0.59{\pm}0.16$ in the control cultures. FUdR treated cells showed significantly higher (p<0.001) AC and NoA means ($2.18{\pm}0.33$ and $2.65{\pm}0.50$). The sex comparison for the frequency of expression indicated that males had significantly higher number of aberrant cells and total number of aberrations in FUdR cultures than the female group in Lohi sheep. The comparison of control cultures was however, not significantly different between the two groups. The regression analysis of FUdR-induced chromosomal fragility data analysis of the fragility data predicted very low ${\beta}$ of 0.325 and 0.412 for AC and NoA respectively. Lohi chromosomes expressed lesions in only 7 and 24 bands in the control and FUdR cultures respectively. The total number of significantly fragile bands in the Lohi genome was only 4. The X-chromosome of the Lohi sheep was highly stable at $5{\mu}g/ml$ FUdR with no fragile sites. The sex comparison for the distribution of fragile sites across the Lohi genome did not reveal any noticeable differences.

Keywords

References

  1. Ali, I. 1993. Identification of individual chromosomes pairs by Giemsa banding in Lohi sheep. M.Sc. Thesis, Department of Animal Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
  2. Adobe creative suite 2000 adobe inc. USA
  3. Babar, M. E., Z. Ahmad and S. Ali. 1991. Studies on the karyotype of Lohi Sheep. Pakistan Vet. J. 11(2):57-61.
  4. Barbi, G., P. Steinbach and W. Vogel. 1984. Nonrandom distribution of methotrexate-induced aberrations on human chromosomes. Detection of further folic acid-sensitive fragile sites. Hum.Genet. 68:290-294. https://doi.org/10.1007/BF00292586
  5. Bhatia, S. and R. Arora. 2005. Biodiversity and conservation of Indian sheep genetic resources - An Overview. Asian-Aust. J. Anim. Sci. 18:1387-1402. https://doi.org/10.5713/ajas.2005.1387
  6. Bohm, U., P. F. Dahm, B. F. McAllister and I. F. Greenbaum. 1995. Identifying chromosomal fragile sites from individuals: a multinomial statistical model. Hum. Genet. 95:249-256.
  7. Carlose, L., D. R. Krumdieck, N. Patricia, Howard Peebles and M. John Optiz. 2005. On the nature of folic acid sensitive fragile sites in human chromosomes. Am. J. Med. Genet. 16(1):23-23. https://doi.org/10.1002/ajmg.1320160105
  8. Greenbaum, I. F. and P. F. Dahm. 1995. FSM version 995 an MSDOS program for the statistical identification of chromosomal fragile sites, User's Guide.
  9. Girish, H., S. N. Sivaselvam, S. M. K. Karthickeyan and R. Saravanan. 2007. Molecular characterization of Nilgiri sheep (Ovis aries) of South India based on microsatellites. Asian-Aust. J. Anim. Sci. 20:633-637. https://doi.org/10.5713/ajas.2007.633
  10. Hirbo, J., A. Muigai, A. Naqvi, E. D. Rege and O. Hanotte. 2006. The genetic diversity of trans-caucasian native sheep breeds. Asian-Aust. J. Anim. Sci. 19:943-952. https://doi.org/10.5713/ajas.2006.943
  11. Long, S. E. 1985. Standard nomenclature for the G-band karyotype of the domestic sheep (Ovis aries). Committee for standardized karyotype of Ovis aries. Hereditas. 103:165-170. https://doi.org/10.1111/j.1601-5223.1985.tb00497.x
  12. Long, S. E. 1997. Chromosome abnormalities in domestic sheep. Review article. J. Appl. Genet. 38:65-76.
  13. Lopez-Corrals, N. L. and M. V. Arruga. 1996. Induction of fragile sites in goats: A preliminary study. Genet. Sel. Evol. 28:129-139. https://doi.org/10.1186/1297-9686-28-2-129
  14. Minitab Statistical Software Release 3.11, Minitab Inc. State College, Pennsylvania, USA.
  15. Nicodemo, D., G. Coppola, A. Pauciullo, G. Cosenza, L. Ramunno, F. Ciotola, V. Perreti, G. P. Di Meo, L. Iannuzzi, J. Rubes and D. Di Berardino. 2007. Mapping fragile-sites in the standard karyotype of River Buffalo (Bubalus bubalus, 2N = 50). Italian J. Anim. Sci. 6(Suppl-2):291-294. https://doi.org/10.4081/ijas.2007.s2.291
  16. Riggs, P. K. and T. Kuczek. 1993. Analysis of aphidicolineinduced chromosome fragile sites in domestic pig (Sus scrofa) Cytogenet cell Genet. 62:110-116. https://doi.org/10.1159/000133452
  17. Ronne, M. 1992. Putative fragile sites in the horse karyotype. Hereditas. 117:127-136. https://doi.org/10.1111/j.1601-5223.1992.tb00166.x
  18. Ruiz-Herrera, A. 2006. Is mammalian chromosome evolution driven by region of genome fragility. Genome Biol. 7(12):R115. https://doi.org/10.1186/gb-2006-7-9-115
  19. Seabright, M. 1971. A rapid banding technique for human chromosomes. Lancet. 2:971-972.
  20. Smeets, D. F. C. M. and G. Merkx. 1990. Neither age nor sex influence the expression of folate sensitvie common fragile sites on human chromosomes. Human Genet. 86:76-78.
  21. Steane, D. E. 1997. Planning livestock resources: Vision-2010, Animal Genetic Resource. In Proc. Internat. Conf. On Animal Production (ICAP), Dec. 2-3, NARC, Islamabad, Pakistan
  22. Stone, D. M., P. N. Jacky, D. D. Hancork and D. J. Prieur. 1991a. Chromosomal fragile site expression in dogs: I. Breed specific differences. Am. J. Med. Genet. 40:214-222. https://doi.org/10.1002/ajmg.1320400219
  23. Stone, M., P. B. Jacky and D. J. Prieur. 1991b. Chromosomal fragile site expression in Boxer dog with mast cell tumor. Am. J. Med. Genet. 40:223-229. https://doi.org/10.1002/ajmg.1320400220
  24. Stone, D. M. and K. E. Stephens. 1993. Bromodeoxyuridine induces chromosomal fragile sites in canine genome. Am. J. Med. Genet. 46:198-202. https://doi.org/10.1002/ajmg.1320460220
  25. Sutherland, 1998. Personal communication.
  26. Yang, M. Y. and S. E. Long. 1993. Folate sensitive common fragile sites in chromosomes of the domestic pig (Sus scrofa). Res. Vet. Sci. 55:231-235. https://doi.org/10.1016/0034-5288(93)90086-U