Asian-Australasian Journal of Animal Sciences

  • 제21권9호
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  • Pages.1324-1329
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  • 2008
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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The Probiotic and Adherence Properties of Lactobacillus reuteri Pg4 Expressing the Rumen Microbial β-Glucanase

  • Yu, B. (Department of Animal Science, National Chung-Hsing University) ;
  • Liu, J.R. (Institute of Biotechnology and Department of Animal Science and Technology, National Taiwan University) ;
  • Hsiao, F.S. (Department of Animal Science, National Chung-Hsing University) ;
  • Lee, T.T. (Department of Life Science, Mingdao University) ;
  • Chiou, P.W.S. (Chung-Jen College of Nursing, Health Science and Management)
  • 투고 : 2007.09.14
  • 심사 : 2007.12.02
  • 발행 : 2008.09.01
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초록

This study was conducted to evaluate the potential of the transformed Lactobacillus reuteri Pg4 (T-Pg4) harboring the ${\beta}$-glucanase gene as a poultry probiotic. The probiotic properties of the T-Pg4 strain were evaluated in vitro by their adherence capability and acid and bile salt tolerance, and were evaluated in vivo by their survival and adhesion in the gastrointestinal tract (GIT) of specific-pathogen-free (SPF) chickens. The results showed that the T-Pg4 strain exhibited resistance to acidic conditions and contact with bile salt, and adhered efficiently to the crop and intestinal epithelial cells of chickens in vitro. The T-Pg4 strain also could survive and colonize the gastrointestinal epithelium of the experimental SPF chickens in vivo. In addition, radial enzyme diffusion was used to demonstrate that the Lactobacillus spp. randomly isolated from the GIT of the SPF chickens fed T-Pg4 possessed ${\beta}$-glucanase secretion capability. These findings have demonstrated that the transformed L. reuteri Pg4 survives transit through the stomach and intestine, and may secrete ${\beta}$-glucanase in the chicken GIT. Therefore, it is suggested that this organism could be used as a multifunctional poultry probiotic.

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