Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Gene Microarray Analysis for Porcine Adipose Tissue: Comparison of Gene Expression between Chinese Xiang Pig and Large White

  • Guo, W. (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Wang, S.H. (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Cao, H.J. (College of Animal Science and Technology, China Agricultural University) ;
  • Xu, K. (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Zhang, J. (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Du, Z.L. (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Lu, W. (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Feng, J.D. (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Li, N. (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Wu, C.H. (College of Animal Science and Technology, China Agricultural University) ;
  • Zhang, L. (National Engineering Research Center for Beijing Biochip Technology)
  • Received : 2006.04.22
  • Accepted : 2006.11.01
  • Published : 2008.01.01
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Abstract

We created a cDNA microarray representing approximately 3,500 pig genes for functional genomic studies. The array elements were selected from 6,494 cDNA clones identified in a large-scale expressed sequence tag (EST) project. These cDNA clones came from normalized and subtracted porcine adipose tissue cDNA libraries. Sequence similarity searches of the 3,426 ESTs represented on the array using BLASTN identified 2,790 (81.4%) as putative human orthologs, with the remainder consisting of "novel" genes or highly divergent orthologs. We used the gene microarray to profile transcripts expressed by adipose tissue of fatty Chinese Xiang pig (XP) and muscley Large White (LW). Microarray analysis of RNA extracted from adipose tissue of fatty XP and muscley LW identified 81 genes that were differently expressed two fold or more. Transcriptional differences of four of these genes, adipocyte fatty acid binding protein (aP2), stearyl-CoA desaturase (SCD), sterol regulatory element binding transcription factor 1 (SREBF1) and lipoprotein lipase (LPL) were confirmed using SYBR Green quantitative RT-PCR technology. Our results showed that high expression of SCD and SREBF1 may be one of the reasons that larger fat deposits are observed in the XP. In addition, our findings also illustrate the potential power of microarrays for understanding the molecular mechanisms of porcine development, disease resistance, nutrition, fertility and production traits.

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