Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Estimate of Characteristics and Manufacture of Blood Substitutes

혈액대용물질의 제조 및 특성 평가

  • Kim, Gi-Beum (Department of Pharmacology, Collage of Veterinary Medicine, Chonbuk National University) ;
  • Park, Jai-Koan (CIC) ;
  • Kim, Seong-Jong (Division of Chemical Engineering, College of Engineering, Chonbuk National University) ;
  • Kim, Jong-Soo (Division of Chemical Engineering, College of Engineering, Chonbuk National University) ;
  • You, Il-Soo (Department of Polymer Nano Science & Technology, College of Engineering, Chonbuk National University) ;
  • Kim, Min-Ho (Department of Thoracic and Cardiovascular Surgery, Chonbuk National University Medical Schools) ;
  • Hong, Chul-Un (Division of Biomedical Engineering, College of Engineering, Chonbuk National University) ;
  • Kim, Jin-Shang (Department of Pharmacology, Collage of Veterinary Medicine, Chonbuk National University) ;
  • Kang, Hyung-Sub (Department of Pharmacology, Collage of Veterinary Medicine, Chonbuk National University)
  • 김기범 (전북대학교 수의과대학 약리학교실) ;
  • 박재관 ;
  • 김성종 (전북대학교 공과대학 화학공학부) ;
  • 김종수 (전북대학교 공과대학 화학공학부) ;
  • 유일수 (전북대학교 공과대학 고분자나노공학과) ;
  • 김민호 (전북대학교 의학전문대학원 흉부외과학교실) ;
  • 홍철운 (전북대학교 공과대학 바이오메디칼공학부) ;
  • 김진상 (전북대학교 수의과대학 약리학교실) ;
  • 강형섭 (전북대학교 수의과대학 약리학교실)
  • Received : 2008.01.24
  • Accepted : 2008.03.10
  • Published : 2008.06.30
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Abstract

The purpose of this study was to enhance gas exchange by producing hemosome, a hemoglobin microencapsulated with phospholipid of egg, and perfluorocarbone(PFC) emulsion solution. In the experiment, stable emulsion solution with 437 nm of mean particle size could be produced by Flusol-DA sonication, and shortening of emulsion time could be attained with higher stability as well. $0.8{\mu}m$ sized hemosome could be produced by microencapsulation of hemoglobin with phospholipid extracted from egg yolk. The pattern of oxygen saturation curve of hemosome was S shape, which is similar to that found in normal blood, and $P_{50}$ was measured to be 24 mmHg. The oxygen saturation in the mixed solution of hemosome and blood in 1:4(V/V%) ratio was similar to that of normal blood, and the same result was found in the mixed solution of PFC emulsion and blood in 1:4(V/V%) ratio.

본 연구는 헤모글로빈을 계란의 인지질로 microencapsulation한 hemosome과 PFC 유화액을 제조하여 산소전달 특성을 향상시키고자 하였다. 실험결과 fluosol-DA를 sonication하여 평균입자크기가 437 nm의 안정성 있는 유화액을 제조할 수 있었으며, glycerol의 농도를 증가시킴으로써 유화시간을 줄이고 안정도를 높일 수 있었다. 소 혈액으로부터 추출한 헤모글로빈을 달걀노른자로부터 추출한 인지질로 microencapsulation하여 직경 $0.8{\mu}m$의 hemosome을 얻을 수 있었다. Hemosome의 산소포화도 곡선은 정상혈액의 적혈구와 같이 S자형을 보였으며 $P_{50}$은 24 mmHg이었다. Hemosome과 혈액을 1:4(V/V%)의 혼합한 혼합용액에서의 산소 포화도는 정상혈액과 유사한 결과를 보였으며, PFC 유화액과 혈액을 1:4(V/V%)로 혼합한 혼합용액에도 정상혈액과 유사한 결과를 보였다.

Keywords

Acknowledgement

Supported by : 한국학술진흥재단

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