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생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Multiple shRNA expressing vector enhances efficiency of gene silencing

  • Song, Jun (Division of Oral Biology and Medicine, UCLA School of Dentistry) ;
  • Giang, An (Division of Oral Biology and Medicine, UCLA School of Dentistry) ;
  • Lu, Yingchun (Division of Oral Biology and Medicine, UCLA School of Dentistry) ;
  • Pang, Shen (Division of Oral Biology and Medicine, UCLA School of Dentistry) ;
  • Chiu, Robert (Division of Oral Biology and Medicine, UCLA School of Dentistry)
  • 발행 : 2008.05.31
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초록

RNA interference (RNAi) is the process of sequence-specific gene silencing. However, RNAi efficiency still needs to be improved for effective inhibition of target genes. We have developed an effective strategy to express multiple shRNAs (small hairpin RNA) simultaneously using multiple RNA Polymerase III (Pol III) promoters in a single vector. Our data demonstrate that multiple shRNAs expressed from Pol III promoters have a synergistic effect in repressing the target gene. Silencing of endogenous cyclophilin A (CypA) or key HIV viral genes by multiple shRNAs results in significant inhibition of the target gene.

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참고문헌

  1. Shi, Y. (2003) Mammalian RNAi for the masses. Trends Genet. 19, 9-12 https://doi.org/10.1016/S0168-9525(02)00005-7
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  7. Sun, D., Melegari, M., Sridhar, S., Rogler, C. E. and Zhu, L. (2006) Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multigene knockdown. BioTechniques 41, 59-63 https://doi.org/10.2144/000112203
  8. Xia, X., Zhou, H. and Xu, Z. (2006) Multiple shRNAs expressed by an inducible pol II promoter can knock down the expression of multiple target genes. BioTechniques 41, 64-68 https://doi.org/10.2144/000112198
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