BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Expression and localization of the spermatogenesis-related gene, Znf230, in mouse testis and spermatozoa during postnatal development

  • Song, Hongxia (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Su, Dan (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Lu, Pan (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Yang, Jiyun (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Zhang, Wei (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Yang, Yuan (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Liu, Yunqiang (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Zhang, Sizhong (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University)
  • 발행 : 2008.09.30
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초록

Znf230, the mouse homologue of the human spermatogenesis-related gene, ZNF230, has been cloned by rapid amplification of cDNA ends (RACE). This gene is expressed predominantly in testis, but its expression in different testicular cells and spermatogenic stages has not been previously analyzed in detail. In the present study, the cellular localization of the Znf230 protein in mouse testis and epididymal spermatozoa was determined by RT-PCR, immunoblotting, immunohistochemistry and immunofluorescence. It is primarily expressed in the nuclei of spermatogonia and subsequently in the acrosome system and the entire tail of developing spermatids and spermatozoa. The results indicate that Znf230 may play an important role in mouse spermatogenesis, including spermatogenic cell proliferation and sperm maturation, as well as motility and fertilization.

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피인용 문헌

  1. Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage-gated channels, methylation/acetylation, and transcription factors 2009, https://doi.org/10.1002/jemt.20785
  2. Unmethylated state of 5′ upstream CpG islands may be necessary but not sufficient for the testis-enriched expression of ZNF230/Znf230 vol.36, pp.2, 2014, https://doi.org/10.1007/s13258-013-0153-x
  3. Replication analysis confirms the association of several variants with acute myeloid leukemia in Chinese population vol.142, pp.1, 2016, https://doi.org/10.1007/s00432-015-2010-6
  4. Human RING finger protein ZNF645 is a novel testis-specific E3 ubiquitin ligase vol.12, pp.5, 2010, https://doi.org/10.1038/aja.2010.54
  5. A new mutant transcript generated in Znf230 exon 2 knockout mice reveals a potential exon structure in the targeting vector sequence vol.45, pp.2, 2013, https://doi.org/10.1093/abbs/gms101
  6. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility vol.37, pp.4, 2014, https://doi.org/10.1590/S1415-47572014005000013