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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Regulation of melanocyte apoptosis by Stathmin 1 expression

  • Zhang, Yan (Department of Biochemistry and Molecular Biology, Zhongshan Medical College, Sun Yat-sen University) ;
  • Xiong, Jianjun (Department of Biochemistry and Molecular Biology, Zhongshan Medical College, Sun Yat-sen University) ;
  • Wang, Jiali (Department of Biochemistry and Molecular Biology, Zhongshan Medical College, Sun Yat-sen University) ;
  • Shi, Xianping (Department of Biochemistry and Molecular Biology, Zhongshan Medical College, Sun Yat-sen University) ;
  • Bao, Guodong (DaAn Gene Co., Ltd., Sun Yat-sen University, Guangzhou, Guangdong, People's Republic of China) ;
  • Zhang, Yang (DaAn Gene Co., Ltd., Sun Yat-sen University, Guangzhou, Guangdong, People's Republic of China) ;
  • Zhu, Zhenyu (Department of Biochemistry and Molecular Biology, Zhongshan Medical College, Sun Yat-sen University)
  • Published : 2008.11.30
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Abstract

Undesirable hyperpigmentation that can arise from increased melanocyte activity may be alleviated by targeting active melanocytes for apoptosis. The role of Stathmin 1 as an important regulator of microtubule dynamics is well documented. The current study examined the potential of Stathmin 1-targeting strategies in eliminating active melanocytes. A vector to overexpress Stathmin 1 and vectors to express three distinct small hairpin RNAs to knockdown Stathmin 1 expression in normal melanocytes were produced and in cell cultures acted accordingly. Both overexpression and knockdown of Stathmin 1 led to a marked increase in melanocyte apoptosis, as indicated by the accumulation of apoptotic cells and increased levels of cleaved caspase-3. Both up- and down-regulation of Stathmin 1 expression inhibited the activity of differentiated melanocytes, as indicated by decreases in both melanin production and tyrosinase activity. Taken together, these results indicate that hyperactive melanocytes can be inhibited by altering Stathmin 1 expression.

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References

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