Archives of Plastic Surgery

  • 제35권3호
  • /
  • Pages.248-254
  • /
  • 2008
  • /
  • 2234-6163(pISSN)
  • /
  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
권호 표지

여러 가지 가교제가 인체 무세포진피의 안정성에 미치는 영향

Effects of Cross-Linking Agents on the Stability of Human Acellular Dermal Matrix

  • 강낙헌 (충남대학교 의과대학 성형외과학교실) ;
  • 윤영묵 (충남대학교 의과대학 성형외과학교실) ;
  • 우종설 (충남대학교 의과대학 성형외과학교실) ;
  • 안재형 (한스 바이오메드) ;
  • 김진영 (한스 바이오메드)
  • Kang, Nak Heon (Department of Plastic & Reconstructive Surgery, College of Medicine, Chungnam National University) ;
  • Yun, Young Mook (Department of Plastic & Reconstructive Surgery, College of Medicine, Chungnam National University) ;
  • Woo, Jong Seol (Department of Plastic & Reconstructive Surgery, College of Medicine, Chungnam National University) ;
  • Ahn, Jae Hyung (Hans Biomed) ;
  • Kim, Jin Young (Hans Biomed)
  • 투고 : 2008.02.15
  • 발행 : 2008.05.10
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose: Human acellular dermal matrix(ADM) is widely used in the treatment of congenital anomalies and soft tissue deficiencies. But it is rapidly degraded in the body and does not provide satisfactory results. There is a need to improve collagen fiber stability through various methods and ultimately regulate the speed of degradation. Methods: The ADMs were added with various cross-linking agents called glutaraldehyde, dimethyl 3,3'-dithiobispropionimidate to produce cross-linked acellular dermal matrices. 1,4-butanediol diglycidyl ether solution was applied with a pH of 4.5 and 9.0, respectively. The stability of cross-linked dermal matrix was observed by measuring the shrinkage temperature and the degradation rates. The cross- and non-cross linked dermis were placed in the rat abdomen and obtained after 8, 12 and 16 weeks. Results: The shrinkage temperature significantly increased and the degradation rate significantly decreased, compared to the control(p<0.05). All of cross-linked dermises were observed grossly in 16 weeks, but most of non-cross linked dermis were absorbed in 8 weeks. Histologically, the control group ADM was found to have been infiltrated with fibroblasts and most of dermal stroma were transformed into the host collagen fibers. However, infiltration of fibroblasts in the experiment was insignificant and the original collagen structure was intact. Conclusion: Collagen cross-linking increases the structural stability and decreases degradation of acellular dermis. Therefore, decrease in body absorption and increase in duration can be expected.

키워드

과제정보

연구 과제 주관 기관 : 충남대학교

참고문헌

  1. Kim HT, Ahn ST, Park JG: Absorption rates of various- thickness human acellular dermal graft(SureDerm$\Re$). J Korean Soc Plast Reconstr Surg 30: 224, 2003
  2. Matsui R, Okura N, Osaki K, Konishi J, Ikegami K, Koide M: Histologic evaluation of skin reconstruction using artificial dermis. Biomaterials 17: 995, 1996 https://doi.org/10.1016/0142-9612(96)84674-6
  3. Na YC, Kang YS, Ryu BS, Yang JY: Reconstruction of soft tissue defect of feet and legs using Alloderm$\Re$. J Korean Soc Plast Reconstr Surg 26: 996, 1999
  4. Castor SA, To WC, Papay FA: Lip augmentation with Alloderm$\Re$ acellular allogenic dermal graft and fat autograft: a comparison with autologous fat injection alone. Aesthetic Plast Surg 23: 218, 1999 https://doi.org/10.1007/s002669900271
  5. Liang HC, Chang Y, Hsu CK, Lee MH, Sung HW: Effects of crosslinking degree of an acellular biological tissue on its tissue regeneration pattern. Biomaterials 25: 3541, 2004 https://doi.org/10.1016/j.biomaterials.2003.09.109
  6. Zeeman R, Dijkstra PJ, van Wachem PB, van Luyn MJ, Hendriks M, Cahalan PT, Feijen J: Crosslinking and modification of dermal sheep collagen using 1,4- butanediol diglycidyl ether. J Biomed Mater Res 46: 424, 1999 https://doi.org/10.1002/(SICI)1097-4636(19990905)46:3<424::AID-JBM16>3.0.CO;2-R
  7. Sung HW, Chang WH, Ma CY, Lee MH: Crosslinking of biological tissues using genipin and/or carbodiimide. J Biomed Mater Res A 64: 427, 2003
  8. Weadock K, Olson RM, Silver FH: Evaluation of collagen crosslinking techniques. Biomater Med Devices Artif Organs 11: 293, 1983 https://doi.org/10.3109/10731198309118815
  9. Olde Damink LH, Dijkstra PJ, van Luyn MJ, van Wachem PB, Nieuwenhuis P, Feijen J: Cross-linking of dermal sheep collagen using hexamethylene diisocyanate. J Mater Sci Mater Med 6: 429, 1995 https://doi.org/10.1007/BF00120286
  10. Harriger MD, Supp AP, Warden GD, Boyce ST: Glutaraldehyde crosslinking of collagen substrates inhibits degradation in skin substitutes grafted to athymic mice. J Biomed Mater Res 35: 137, 1997 https://doi.org/10.1002/(SICI)1097-4636(199705)35:2<137::AID-JBM1>3.0.CO;2-O
  11. Charulatha V, Rajaram A: Dimethyl 3,3'-dithiobispropionimidate: a novel crosslinking reagent for collagen. J Biomed Mater Res 54: 122, 2001 https://doi.org/10.1002/1097-4636(200101)54:1<122::AID-JBM15>3.0.CO;2-N
  12. Zeeman R, Dijkstra PJ, van Wachem PB, van Luyn MJA, Hendriks M, Cahalan PT, Feijen J: Successive epoxy and carbodiimide cross-linking of dermal sheep collagen. Biomaterials 20: 921, 1999 https://doi.org/10.1016/S0142-9612(98)00242-7
  13. van Wachem PB, Zeeman R, Dijkstra PJ, Feijen J, Hendriks M, Cahalan PT, van Luyn MJ: Characterization and biocompatibility of epoxy-crosslinked dermal sheep collagens. J Biomed Mater Res 47: 270, 1999 https://doi.org/10.1002/(SICI)1097-4636(199911)47:2<270::AID-JBM18>3.0.CO;2-D
  14. Park DS, Kim HK, Kim SH, Kim SH, Lee TJ: Comparision of survial of human dermis, Alloderm$\Re$ and Terudermis$\Re$ graft in nude mouse. J Korean Soc Plast Reconstr Surg 29: 188, 2002
  15. Sclafani AP, Romo T 3rd, Jacono AA, McCormick SA, Cocker R, Parker A: Evaluation of acellular dermal graft(Alloderm$\Re$) sheet for soft tissue augmentation: a 1-year follow-up of clinical observations and histological findings. Arch Facial Plast Surg 3: 101, 2001 https://doi.org/10.1001/archfaci.3.2.101