한국어병학회지 (Journal of fish pathology)

  • 제21권1호
  • /
  • Pages.13-20
  • /
  • 2008
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

수온별 Red Sea Bream Iridovirus (RSIV) 인위감염에 따른 돌돔의 누적폐사 및 Heat Shock Protein (HSP) 70의 동정

Cumulative Mortality in Striped Beakperch, Oplegnathus fasciatus Infected with Red Sea Bream Iridovirus (RSIV) at Different Water Temperature and Identification of Heat Shock Protein 70

  • 김석렬 (전남대학교 식품∙수산생명의학부) ;
  • 정병문 (전남대학교 식품.수산생명의학부) ;
  • 정성주 (전남대학교 식품.수산생명의학부) ;
  • 키타무라 신이치 (에히메대학교 연안환경과학연구센터) ;
  • 김두운 (전남대학교 식품.수산생명의학부) ;
  • 김도형 (전남대학교 식품.수산생명의학부) ;
  • 오명주 (전남대학교 식품.수산생명의학부)
  • Kim, Seok-Ryel (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Jeong, Byeong-Mun (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Jung, Sung-Ju (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Kitamura, Shin-Ichi (Center for Marine Environment Studies (CMES), Ehime University) ;
  • Kim, Du-woon (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Kim, Do-Hyung (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Oh, Myung-Joo (Division of Food Science and Aqualife Medicine, Chonnam National University)
  • 발행 : 20080400
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 RSIV의 수온에 따른 병원성을 규명하기 위한 연구의 일환으로 수온별로 RSIV를 인위적으로 감염시킨 돌돔의 누적폐사를 관찰하고, 다른 온도에서 사육된 돌돔의 온도 스트레스 단백질인 HSP이 관여하는지를 확인하고자, 돌돔의 HSP 유전자의 염기서열 일부를 밝혔다. 각온도별 감염실험에서 17°C와 20°C의 경우 폐사가 발생하지 않았으나, 25°C 및 27°C 수조에서는 각각 90%와 80%의 누적 폐사율을 보였다. 돌돔에서 확인된 HSP 유전자의 일부는 넙치에서 확인된 HSP70와 97%의 homology를 나타내 돌돔의 HSP70으로 판단되었다.

This study evaluates the pathogenicity in striped beakperch, Oplegnathus fasciatus infected with red sea bream iridovirus (RSIV) at different water temperature (17°C, 20°C, 25°C and 27°C). When the fish group was infected with RSIV at 17°C and 20°C, cumulative mortality did not show any significant difference with control group. In contrast, the case at 25°C and 27°C, cumulative mortality reached more than 80%. However, RSIV was detected from all of the fish in each temperature. To confirm a relationship between temperature change and heat shock protein (HSP), partial HSP70 cDNA was isolated from striped beakperch.

키워드

참고문헌

  1. Airaksinen, S., Rabergh, C.M., Sistonen, L., Nikinmaa, M. (1998): Effects of heat shock and hypoxia on protein synthesis in rainbow trout (Oncorhynchus mykiss) cells. J. Exp. Biol. 201:2543-2551
  2. Basu, N., Todgham, A.E., Ackerman, P.A., Bibeau, M.R., Nakano, K., Schulte, P.M., Iwama, G.K. (2002): Heat shock proteins genes and their functional significance in fish. Gene, 295:173-183 https://doi.org/10.1016/S0378-1119(02)00687-X
  3. Broome, C.S., Kayani, A.C., Palomero, J., Dillmann, W.H., Mestril, R., Jackson, M.J., McArdle, A. (2006): Effect of lifelong overexpression of HSP70 in skeletal muscle on age-related oxidative stress and adaptation after nondamaging contractile activity. FASEB J. 20:1549-1551 https://doi.org/10.1096/fj.05-4935fje
  4. Deane, E.E., Zhou, L.R., Woo,,N.Y.S. (2006): Cortisol can be pro- or anti-apoptotic in sea bream cells: potential role of HSP70 induction for cytoprotection. Mol. Cell. Endocrinol., 259:57-64 https://doi.org/10.1016/j.mce.2006.08.006
  5. Gamperl, A.K., Vijayan, M.M., Pereira, C., Farrell, A.P. (1998): Beta-receptors and stress protein 70 expression in hypoxic myocardium of rainbow trout and chinook salmon. Am. J. Physiol. 274:428-436
  6. Go, J., Lancaster, M., Deece, K., Dhungyel, O., and Whittington, R. (2002): The molecular epidemiology of iridovirus in Murray cod (Maccullochella peelii peelii) and dwarf gourami (Colisa lalia) from distant biogeographical regions suggests a link between trade in ornamental fish and emerging iridoviral diseases. Mol. Cell. Probes, 16:137-151 https://doi.org/10.1006/mcpr.2001.0400
  7. Heredia-Middleton, P., Brunelli, J., Drew, R.E., Thorgaard, G.H.(2008): Heat shock protein (HSP70) RNA expression differs among rainbow trout (Onchrhynchus mykiss) clonal lines. Comp. Biochem. Physiol. B, in press
  8. Iwama, G.K., Thomas, P., Vijayan, M.M., Forsyth, R.B. (1998): Stress proteins expression in fish. Rev. Fish Biol. Fish., 8:35-56 https://doi.org/10.1023/A:1008812500650
  9. Iwama, G.K., Vijayan, M.M., Forsyth, R.B., Ackerman, P.A. (1999): Heat shock proteins and physiological stress in fish. Am. Zool., 39:901-909 https://doi.org/10.1093/icb/39.6.901
  10. Jung, S.J., Oh, M.J. (2000): Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Tem-minck et Schlegel), in southern coastal areas of theKorean peninsula. J. Fish Dis., 23:223-226 https://doi.org/10.1046/j.1365-2761.2000.00212.x
  11. Kim, W.S., Kim, K.H., Kim, C.S., Kim, Y.J., Jung, S.J., Jung, T.S., Kitamura, S.I., Yoshimizu, M. and Oh, M.J. (2003): The infection of irido- like virus in cultured turbot (Scophthalmus maximus). J. Fish Pathol., 16:153-159
  12. Kim, Y.J., Jung, S.J., Choi, T.J., Kim, H.R., Rajendran, KV., Oh, M.J.(2002): PCR amplification and sequence analysis of irido-like virus infecting fish in Korea. J. Fish Dis., 25:121-124 https://doi.org/10.1046/j.1365-2761.2002.00345.x
  13. Kitamura, S.I., Ko, J.Y., Lee, W.L., Kim, S.R., Song, J.Y., Kim, D.K., Jung, S.J., Oh, M.J. (2007): Seasonal prevalence of lymphocystis disease virus and aquabirnavirus in Japanese flounder, Paralichthys olivaceus and blue mussel, Mytilus galloprovincialis. Aquaculture, 266:26-31 https://doi.org/10.1016/j.aquaculture.2007.02.034
  14. Kiang, J.G., Tsokos, G.C. (1998): Heat shock proteins 70 kDa: Molecular biology, biochemistry and physiology. Pharmacol. Ther. 80:183-201 https://doi.org/10.1016/S0163-7258(98)00028-X
  15. Lyu, J.H., Jeong, J.B., Kim, H.Y., Jun L.J., Cho, H.J., Lee, J.W. and Jeong, H.D. (2006): Detection and distribution of iridoviruses in five freshwater ornamental fish species. J. Fish Pathol., 19:197-206
  16. Matsuoka, S., Inouye, K. and Nakajima, K. (1996): Cultured fish species affected by red sea bream iridoviral disease from 1991 to 1995. Fish Pathol. 31:233-234 https://doi.org/10.3147/jsfp.31.233
  17. Mayr, M., Kiechl, S., Willeit, J., Wick, G., Xu, Q. (2000): Infections, immunity, and atherosclerosis: associations of antibodies to Chlamydia pneumoniae, Helicobacter pylori, and cytomegalovirus with immune reactions to heat shock protein 60 and carotid or femoral atherosclerosis. Circulation 102:833-839 https://doi.org/10.1161/01.CIR.102.8.833
  18. McConkey, D.J. (1998): Biochemical determinants of apoptosis and necrosis. Toxicol. Lett., 99:157-168 https://doi.org/10.1016/S0378-4274(98)00155-6
  19. Misra, S., Zafarullah, M., Price-Haughey, J., Gedamu, L. (1989): Analysis of stress-induced gene expression in fish cell lines exposed to heavy meals and heat shock. Biochim. Biophys. Acta 1007:325-333 https://doi.org/10.1016/0167-4781(89)90155-3
  20. Mosharrof, H., Kim, S.R., Kitamura, S.I., Kim, D., Jung, S.J., Nishizawa, T., Yoshimizu, M., Oh, M.J. (2008): Lymphocystis disease virus (LCDV) persistently exists in epidermis of olive flounder (Paralichthys olivaceus) at low temperature. J. Fish Dis., in press
  21. Mosharrof, H., Kim, S.R., Oh, M.J. (2007): The lymphocystis diseases in the olive flounder, Paralichthys olivaceus. Univ. J. Zool. Rajshahi Univ., 26:59-62
  22. Oh, M.J., Jung, S.J. and Kim, Y.J. (1999): Detection of RSIV (Red Sea Bream Iridovirus) in the cultured marine fish by the polymerase chain reaction. J. Fish Pathol., 12: 66-69
  23. Oh, M.J., Kitamura, S.I., Kim, W.S., Park, M.K., Jung, S.J., Miyadai, T., Ohtani, M. (2006): Susceptibility of marine fish species to a megalocytivirus, turbot iridovirus, isolated from turbot, Psetta maximus (L.). J. Fish Dis., 29:415-421 https://doi.org/10.1111/j.1365-2761.2006.00734.x
  24. Steward, G., Snewin, R.V.A., Walzl, G., Hussell, T., Tormay, P., O'Gaora, P., Goyal, M., Betts, J., Brown, I.N., Young, D.B. (2001): Over expression of heat-shock proteins reduces survival of Mycobacterium tuberculosis in the chroni phase of infection. Nat. Med. 7:732-737 https://doi.org/10.1038/89113
  25. Van Regennortel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Carstens, E.B., Estes, M.K., Lemon, S.M., Maniloff, J., Mayo, M.A., McGeoch, D.J., Pringle, C.R. and Wickner, E.B. (2000): Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses., Academic Press, New York
  26. Wagner, M., Hermanns, I., Bittinger, F., Kirkpatrick, C.J. (1999): Induction of stress proteins in human endothelial cells by heavy metal ions and heat shock. Am. J. Physiol. 277:1026-1033